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25-Hydroxycholecalciferol monohydrate
25-Hydroxycholecalciferol monohydrate
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name 25-Hydroxycholecalciferol monohydrate
Price:
CAS No.: 63283-36-3
Catalog No.: CFN94017
Molecular Formula: C27H44O2
Molecular Weight: 400.64 g/mol
Purity: >=98%
Type of Compound: Miscellaneous
Physical Desc.: Powder
Source: From milk.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS
Similar structural: Comparison
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Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
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Biological Activity
Description: 25-Hydroxycholecalciferol supplementation at higher doses improved growth performance and decreased inflammatory gene IL-1β mRNA amounts in the liver post-LPS injection.
Targets: IL Receptor
25-Hydroxycholecalciferol monohydrate Description
Source: From milk.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

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After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.496 mL 12.48 mL 24.9601 mL 49.9201 mL 62.4002 mL
5 mM 0.4992 mL 2.496 mL 4.992 mL 9.984 mL 12.48 mL
10 mM 0.2496 mL 1.248 mL 2.496 mL 4.992 mL 6.24 mL
50 mM 0.0499 mL 0.2496 mL 0.4992 mL 0.9984 mL 1.248 mL
100 mM 0.025 mL 0.1248 mL 0.2496 mL 0.4992 mL 0.624 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Animal Research:
Poult Sci. 2014 Aug;93(8):1951-6.
25-Hydroxycholecalciferol supplementation improves growth performance and decreases inflammation during an experimental lipopolysaccharide injection.[Pubmed: 24931970 ]
Three experiments were conducted to study the effects of 25-hydroxycholecalciferol (25-Hydroxycholecalciferol monohydrate )supplementation on BW gain, IL-1β, and 1α-hydroxylase mRNA expression in different organs of broiler chickens following a lipopolysaccharide (LPS) injection.
METHODS AND RESULTS:
In experiment I, birds were fed a basal diet supplemented with either cholecalciferol (3,000 IU/kg) or 25-hydroxycholecalciferol (69 µg/kg). At 21 and 35 d of age, birds were injected with LPS. Post-LPS injection, birds supplemented with 25-hydroxycholecalciferol gained approximately 2.5% (P = 0.03) and 3.8% (P < 0.01), respectively, more BW than the birds supplemented with cholecalciferol over the 24-h period. In experiment II, birds were fed basal diets supplemented with 25-hydroxycholecalciferol at 6.25, 25, and 50 µg/kg of feed or cholecalciferol at 250 IU/kg of feed. At 35 d of age, birds were injected with LPS. Birds fed 25-hydroxycholecalciferol at 25 and 50 µg/kg and injected with LPS had approximately 7-fold and 3-fold less (P = 0.010) IL-1β mRNA in the liver compared with those birds fed 6.25 µg/kg of 25-hydroxycholecalciferol and the cholecalciferol (250 IU/kg) group. In experiment III, birds were fed a basal diet supplemented with either cholecalciferol (3,000 IU/kg) or 25-hydroxycholecalciferol (69 µg/kg). At 28 d of age, birds were fed 25-hydroxycholecalciferol and injected with LPS had 1.1-fold less (P < 0.01) IL-1β mRNA in the liver than the cholecalciferol-fed group. After an LPS injection, birds supplemented with 25-hydroxycholecalciferol had increased 1α-hydroxylase mRNA amounts in the liver (P = 0.07).
CONCLUSIONS:
In conclusion, 25-hydroxycholecalciferol supplementation at higher doses improved growth performance and decreased inflammatory gene IL-1β mRNA amounts in the liver post-LPS injection.
Structure Identification:
Biochemical and Biophysical Research Communications, 1980, 95(1):177-181.
Isolation and identification of 25-hydroxy-24-oxocholecalciferol: A metabolite of 25-hydroxycholecalciferol[Reference: WebLink]
A metabolite of 25-hydroxycholecalciferol(25-Hydroxycholecalciferol monohydrate ) has been isolated in pure form from chicken kidney homogenates.
METHODS AND RESULTS:
It has been identified as 25-hydroxy-24-oxocholecalciferol by means of ultraviolet absorption spectrophotometry, mass spectrometry, infrared spectrometry, nuclear magnetic resonance spectrometry, and specific chemical reactions.
J Agric Food Chem. 1979 May-Jun;27(3):650-2.
25-Hydroxycholecalciferol in cow milk as determined by high-performance liquid chromatography[Reference: WebLink]

METHODS AND RESULTS:
A high-performance liquid Chromatographic (LC) procedure was developed for the determination of 25-hydroxycholecalciferol (25-Hydroxycholecalciferol monohydrate,25-OH-D3) in cow milk. The procedure involved extraction with an ethanol-ether mixture, a set of solvent partitions, adsorption chromatography on silica gel, a partition chromatography on diatomaceous earth support, and final determination by reversed-phase LC on a C18 bonded microparticulate silica column using a 254-nm fixed wavelength detector. The method was quantitative at the 10-ppb level and capable of detection at the 2-3-ppb level.
CONCLUSIONS:
The method was applicable to milk and colostrum. The endogenous level of 25-OH-D3 in the milk was below the detection limit. It was very significant that, even in cows whose serum concentrations were elevated fivefold after treatment with 25-OH-D3, the concentration in the milk was below the detection level.
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