|Source:||The barks of Eucommia ulmoides Oliver|
|Biological Activity or Inhibitors:||1. (2R,3S)-Dihydrodehydroconiferyl alcohol can induce neurite outgrowth and enhance NGF-induced neurite outgrowth from PC12 cells by amplifying up-stream steps such as MAPK and PKC.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.7747 mL||13.8735 mL||27.7469 mL||55.4939 mL||69.3674 mL|
|5 mM||0.5549 mL||2.7747 mL||5.5494 mL||11.0988 mL||13.8735 mL|
|10 mM||0.2775 mL||1.3873 mL||2.7747 mL||5.5494 mL||6.9367 mL|
|50 mM||0.0555 mL||0.2775 mL||0.5549 mL||1.1099 mL||1.3873 mL|
|100 mM||0.0277 mL||0.1387 mL||0.2775 mL||0.5549 mL||0.6937 mL|
Arch Pharm Res. 2005 Dec;28(12):1337-40.
|Induction of neurite outgrowth by (-)-(7R, 8S)-dihydrodehydrodiconiferyl alcohol from PC12 cells.[Pubmed: 16392665]|
|A lignan derivative, (-)-(7R, 8S)-dihydrodehydrodiconiferyl alcohol ((2R,3S)-Dihydrodehydroconiferyl alcohol,DHDA), was isolated from Kalopanax septemlobus L. and was observed to have neuritogenic activity. DHDA at 50 microM caused a marked induction of neurite outgrowth and an enhancement of nerve growth factor (NGF)-mediated neurite outgrowth from PC12 cells. However, it did not exhibit any neurotrophic action. At 50 microM, DHDA enhanced NGF-induced neurite-bearing activity. This activity was partially blocked by the mitogen-activated protein kinase (MAPK) inhibitor PD98059 and by GF109203X, a protein kinase C (PKC) inhibitor. These results suggest that DHDA can induce neurite outgrowth and enhance NGF-induced neurite outgrowth from PC12 cells by amplifying up-stream steps such as MAPK and PKC.|