|Source:||The herbs of Onychium japonicum|
|Biological Activity or Inhibitors:||1. 3,4-Dihydroxybenzoic acid exhibits scavenging actions against the 1,1-diphenyl-2-picrylhydrazyl radical, the superoxide anion, and the hydroxyl radical.
2. 3,4-Dihydroxybenzoic acid isolates from a green alga protects human keratinocytes against UVB-induced oxidative stress and apoptosis.
3. The 3,4-Dihydroxybenzoic acid treatment resulted in 33.3, 65.0, 76.7 and 85.0% hatch inhibition at 0.125, 0.25, 0.5 and 1.0 mg/ml, respectively, 3 days after incubation.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||6.4893 mL||32.4465 mL||64.8929 mL||129.7859 mL||162.2323 mL|
|5 mM||1.2979 mL||6.4893 mL||12.9786 mL||25.9572 mL||32.4465 mL|
|10 mM||0.6489 mL||3.2446 mL||6.4893 mL||12.9786 mL||16.2232 mL|
|50 mM||0.1298 mL||0.6489 mL||1.2979 mL||2.5957 mL||3.2446 mL|
|100 mM||0.0649 mL||0.3245 mL||0.6489 mL||1.2979 mL||1.6223 mL|
Appl Biochem Biotechnol. 2014 Mar;172(5):2582-92.
|Protective effect of 3,4-dihydroxybenzoic acid isolated from Cladophora wrightiana Harvey against ultraviolet B radiation-induced cell damage in human HaCaT keratinocytes.[Pubmed: 24414942]|
|The aim of the present study was to elucidate the protective properties of 3,4-Dihydroxybenzoic acid (DBA) isolated from Cladophora wrightiana Harvey (a green alga) against ultraviolet B (UVB)-induced damage to human HaCaT keratinocytes. 3,4-Dihydroxybenzoic acid exhibited scavenging actions against the 1,1-diphenyl-2-picrylhydrazyl radical, the superoxide anion, and the hydroxyl radical. Furthermore, 3,4-Dihydroxybenzoic acid decreased the levels of intracellular reactive oxygen species generated by hydrogen peroxide or UVB treatment of the cells. 3,4-Dihydroxybenzoic acid also decreased the UVB-augmented levels of phospho-histone H2A.X and the extent of comet tail formation, which are both indications of DNA damage. Taken together, these results demonstrate that 3,4-Dihydroxybenzoic acid isolated from a green alga protects human keratinocytes against UVB-induced oxidative stress and apoptosis.|
Microb Pathog. 2013 Jun-Jul;59-60:52-9.
|Nematicidal activity of 3,4-dihydroxybenzoic acid purified from Terminalia nigrovenulosa bark against Meloidogyne incognita.[Pubmed: 23603737]|
|In this study, the 3,4-Dihydroxybenzoic acid (3,4-DHBA) from Terminalia nigrovenulosa bark (TNB) was purified and its in vitro nematicidal activity was investigated against Meloidogyne incognita. The purification of 3,4-Dihydroxybenzoic acid used a silica gel column and Sephadex LH-20 chromatography combined with thin-layer chromatography and high performance liquid chromatography. Structural identification of the 3,4-Dihydroxybenzoic acid was conducted using (1)H nuclear magnetic resonance (NMR), (13)C NMR, and liquid chromatography time-of-flight mass spectrometry. Nematicidal activity bioassays revealed that 3,4-Dihydroxybenzoic acid treatment resulted in 33.3, 47.5, 72.5 and 94.2% J2 mortality at 0.125, 0.25, 0.5 and 1.0 mg/ml, respectively after 12 h incubation. J2 mortality was increased significantly (P < 0.0001) with increasing incubation time in the range of 54.2-94.2% from 3 to 9 h after incubation with 3,4-Dihydroxybenzoic acid (1.0 mg/ml), but with no significant difference observed where the incubation time was increased from 9 to 12 h. The 3,4-Dihydroxybenzoic acid treatment resulted in 33.3, 65.0, 76.7 and 85.0% hatch inhibition at 0.125, 0.25, 0.5 and 1.0 mg/ml, respectively, 3 days after incubation. Changes in the shape of the eggs were determined after incubation for 1 day with a 3,4-Dihydroxybenzoic acid concentration of 1.0 mg/ml.|