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3,5-Cycloergosta-6,8(14),22-triene
3,5-Cycloergosta-6,8(14),22-triene
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name 3,5-Cycloergosta-6,8(14),22-triene
Price:
CAS No.: 24352-51-0
Catalog No.: CFN98257
Molecular Formula: C28H42
Molecular Weight: 378.6 g/mol
Purity: >=98%
Type of Compound: Steroids
Physical Desc.: Powder
Source: From Poria cocos(Schw.)Wolf
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF    Manual
Similar structural: Comparison (Web)  (SDF)
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Description: Reference standards.
3,5-Cycloergosta-6,8(14),22-triene Description
Source: From Poria cocos(Schw.)Wolf
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.6413 mL 13.2066 mL 26.4131 mL 52.8262 mL 66.0328 mL
5 mM 0.5283 mL 2.6413 mL 5.2826 mL 10.5652 mL 13.2066 mL
10 mM 0.2641 mL 1.3207 mL 2.6413 mL 5.2826 mL 6.6033 mL
50 mM 0.0528 mL 0.2641 mL 0.5283 mL 1.0565 mL 1.3207 mL
100 mM 0.0264 mL 0.1321 mL 0.2641 mL 0.5283 mL 0.6603 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Structure Identification:
Journal of the American Chemical Society, 1952, 74(21):469-74.
An i-Steroid Hydrocarbon from Ergosterol1.[Reference: WebLink]

METHODS AND RESULTS:
Reinvestigation of a hydrocarbon resulting from dehydration of ergosterol with either phosphorus oxychloride or p-toluenesulfonyl chloride in pyridine and heretofore regarded as an ergostatetraene has shown that the substance is 3,5-cyclo-Δ6, 8(14), 22-ergostatriene (3,5-Cycloergosta-6,8(14),22-triene, V), the first known i-steroid in the ergosterol series. Reaction of the hydrocarbon with hydrogen chloride and its acid-catalyzed hydration result in opening of the cyclopropane ring with formation of mixtures of ergosteryl chlorides or of ergosterol isomers; ergosterol-B1 of reasonable purity has been isolated from the product of hydration. A hydrocarbon, first described by Windaus, resulting from acid-catalyzed dehydration of 3α- and 3β-hydroxy-Δ4,7,22-ergostatriene was investigated to see if it corresponded to a highly dextrorotatory, alumina-isomerized impurity accompanying the i-steroid V.
CONCLUSIONS:
The results indicate that the Windaus hydrocarbon is Δ4,6,8(14),22-ergostatetraene (XIV). Formulas attributed earlier to hydrocarbons resulting from dehydration of epialloergosterol (NaOAc-Ac2O, see III) and of lumisterol (C10-epimer of III) are consistent with present evidence. "Ergosterol-E" is shown to consist largely of a mixture of dihydroergosterols.
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