|Source:||The seeds of Glycine max|
|Biological Activity or Inhibitors:||1. 6,7,4'-Trihydroxyisoflavone has antioxidant activity.
2. 6,7,4'-Trihydroxyisoflavone, is a novel inhibitor of PKCα in suppressing solar UV-induced matrix metalloproteinase 1.
3. 6,7,4'-trihydroxyisoflavone, suppresses adipogenesis in 3T3-L1 preadipocytes via ATP-competitive inhibition of PI3K.
4. 6,7,4'-trihydroxyisoflavone inhibits HCT-116 human colon cancer cell proliferation by targeting CDK1 and CDK2.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||3.701 mL||18.5048 mL||37.0096 mL||74.0192 mL||92.5241 mL|
|5 mM||0.7402 mL||3.701 mL||7.4019 mL||14.8038 mL||18.5048 mL|
|10 mM||0.3701 mL||1.8505 mL||3.701 mL||7.4019 mL||9.2524 mL|
|50 mM||0.074 mL||0.3701 mL||0.7402 mL||1.4804 mL||1.8505 mL|
|100 mM||0.037 mL||0.185 mL||0.3701 mL||0.7402 mL||0.9252 mL|
Int J Mol Sci. 2014 Nov 19;15(11):21419-32. doi: 10.3390/ijms151121419.
|The daidzein metabolite, 6,7,4'-Trihydroxyisoflavone, is a novel inhibitor of PKCα in suppressing solar UV-induced matrix metalloproteinase 1.[Pubmed: 25415304]|
|In this study, we investigated the effect of 6,7,4'-Trihydroxyisoflavone (6,7,4'-THIF), a major metabolite of daidzein, against solar UV (sUV)-induced matrix metalloproteinases (MMPs) in normal human dermal fibroblasts. MMPs play a critical role in the degradation of collagen in skin, thereby accelerating the aging process of skin. The mitogen-activated protein/extracellular signal-regulated kinase (MEK)/extracellular signal-regulated kinase (ERK), mitogen-activated protein kinase (MKK)3/6/p38 and MKK4/c-Jun N-terminal kinases (JNK) signaling pathways are known to modulate MMP-1 function, and their activation by sUV was significantly reduced by 6,7,4'-Trihydroxyisoflavone pretreatment. Our results also indicated that the enzyme activity of protein kinase C (PKC)α, an upstream regulator of MKKs signaling, is suppressed by 6,7,4'-Trihydroxyisoflavone using the in vitro kinase assay. Furthermore, the direct interaction between 6,7,4'-Trihydroxyisoflavone and endogenous PKCα was confirmed using the pull-down assay. Not only sUV-induced MMP-1 expression, but also sUV-induced signaling pathway activation were decreased in PKCα knockdown cells. Overall, we elucidated the inhibitory effect of 6,7,4'-Trihydroxyisoflavone on sUV-induced MMPs and suggest PKCα as its direct molecular target.|
Mol Nutr Food Res. 2013 Aug;57(8):1446-55. doi: 10.1002/mnfr.201200593. Epub 2013 Jun 4.
|A metabolite of daidzein, 6,7,4'-trihydroxyisoflavone, suppresses adipogenesis in 3T3-L1 preadipocytes via ATP-competitive inhibition of PI3K.[Pubmed: 23737351]|
|Daidzein is one of the major soy isoflavones. Following ingestion, daidzein is readily metabolized in the liver and converted into hydroxylated metabolites. One such metabolite is 6,7,4'-Trihydroxyisoflavone (6,7,4'-THIF), which has been the focus of recent studies due to its various health benefits, however, its anti-adipogenic activity has not been investigated. Our objective was to determine the effects of 6,7,4'-Trihydroxyisoflavone on adipogenesis in 3T3-L1 preadipocytes and elucidate the mechanisms of action involved.
Our results suggest that 6,7,4'-Trihydroxyisoflavone suppresses adipogenesis in 3T3-L1 preadipocytes by directly targeting PI3K. Soy isoflavones like 6,7,4'-Trihydroxyisoflavone may have potential for development into novel treatment strategies for chronic obesity.
Carcinogenesis. 2011 Apr;32(4):629-35. doi: 10.1093/carcin/bgr008. Epub 2011 Jan 21.
|6,7,4'-trihydroxyisoflavone inhibits HCT-116 human colon cancer cell proliferation by targeting CDK1 and CDK2.[Pubmed: 21258042]|
|Collectively, these results suggest that CDK1 and CDK2 are potential molecular targets of 6,7,4'-Trihydroxyisoflavone to suppress HCT-116 cell proliferation in vitro and in vivo. These findings provide insight into the biological actions of 6,7,4'-Trihydroxyisoflavone and might establish a molecular basis for the development of new cancer therapeutic agents.|