| In vitro: |
| Fitoterapia. 2014 Dec;99:276-83. | | Inhibition of hepatitis C virus replication by chalepin and pseudane IX isolated from Ruta angustifolia leaves.[Pubmed: 25454460 ] |
METHODS AND RESULTS:
We isolated six compounds, chalepin, scopoletin, γ-fagarine, Arborinine, kokusaginine and pseudane IX. Among them, chalepin and pseudane IX showed strong anti-HCV activities with 50% inhibitory concentration (IC₅₀) of 1.7 ± 0.5 and 1.4 ± 0.2 μg/ml, respectively, without apparent cytotoxicity. Their anti-HCV activities were stronger than that of ribavirin (2.8 ± 0.4 μg/ml), which has been widely used for the treatment of HCV infection.
CONCLUSIONS:
Mode-of-action analyses revealed that chalepin and pseudane IX inhibited HCV at the post-entry step and decreased the levels of HCV RNA replication and viral protein synthesis.
We also observed that Arborinine, kokusaginine and γ-fagarine possessed moderate levels of anti-HCV activities with IC₅₀ values being 6.4 ± 0.7, 6.4 ± 1.6 and 20.4 ± 0.4 μg/ml, respectively, whereas scopoletin did not exert significant anti-HCV activities at 30 μg/ml. | | Fitoterapia. 2004 Jul;75(5):510-3. | | Antibacterial activity and cytotoxicity of extractives from Ravenia spectabilis.[Pubmed: 15261391 ] |
METHODS AND RESULTS:
A methanolic extract of Ravenia spectabilis, an isolated alkaloid, Arborinine plus a fraction comprising Arborinine and gamma-fagarine (VLC), showed mild to significant in vitro antibacterial activity.
CONCLUSIONS:
In a brine shrimp lethality bioassay, the extract and the fraction were found to exhibit moderate cytotoxicity having LC50 of 76.26 microg/ml and 14.98 microg/ml, respectively. | | Fitoterapia. 2001 Jun;72(5):538-43. | | Antifeedant constituents from Fagara macrophylla.[Pubmed: 11429249] |
METHODS AND RESULTS:
Analysis of the polar fractions of an EtOH extract obtained from the bark of the African medicinal plant Fagara macrophylla led to the isolation and identification of the alkaloids oblongine (6), tembetarine (7) and magnoflorine (8) and the flavonoid hesperidin (9). These compounds, together with other metabolites (1--5) previously isolated from F. macrophylla, were tested for antifeedant activity in a binary-choice bioassay. The acridone alkaloid xanthoxoline (4) was found to have a potent antifeedant activity against larvae of both Spodoptera frugiperda and S. littoralis.
CONCLUSIONS:
1-Hydroxy-3-methoxy-N-methyl-acridone (2), Arborinine (3), tembetarine (7) and magnoflorine (8) were antifeedant against S. frugiperda. |
|
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)