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More articles cited ChemFaces products.
The Korea Society of Pharmacognosy.2014Phytother Res.2015 Apr 17.Int J Mol Sci. 2014 May 13;15(5):8443-57.Sci Rep. 2017 Jun 12;The Korea Society of Pharmacognosy.2014
J Pharm Biomed Anal.2018 Mar 20;Phytother Res.2018 Jan 29.Phytomedicine2015 March 20.Mol Cells.2015 Sep 30;38(9):765-72.BMC Complement Altern Med.2017 Aug 9;
Tumour Biol.2015 Jun 26Molecules. 2018 Jan 24;Nat Prod Sci.2016 Jun;22(2)Advance Publication2015 Aug 18
Our products had been exported to the following research institutions and universities, And still growing.
Universidade Católica Portuguesa (Portugal)University of Brasilia (Brazil)Nanjing University of Chinese Me... (China)Tokyo Woman's Christian University (Japan)
Sapienza University of Rome (Italy)Stanford University (USA)Universidade Federal de Pernambu... (Brazil)St. Jude Children Research Hospi... (USA)
Georgia Institute of Technology (USA)Helmholtz Zentrum München (Germany)Universidad Miguel Hernández (Spain)
||Caffeine has anxiolytic-like activity, can have both positive and negative effects on anxiety. It inhibits glucose transport by binding at the GLUT1 nucleotide-binding site. |
||Estrogen receptor | GLUT | Progestogen receptor|
|Am J Physiol Cell Physiol. 2015 Feb 25 |
|Caffeine inhibits glucose transport by binding at the GLUT1 nucleotide-binding site.[Pubmed: 25715702]|
|Glucose transporter 1 (GLUT1) is the primary glucose transport protein of the cardiovascular system and astroglia. A recent study proposes that Caffeine uncompetitive inhibition of GLUT1 results from interactions at an exofacial GLUT1 site. Intracellular ATP is also an uncompetitive GLUT1 inhibitor and shares structural similarities with Caffeine, suggesting that Caffeine acts at the previously characterized endofacial GLUT1 nucleotide-binding site.
METHODS AND RESULTS:
We tested this by confirming that Caffeine uncompetitively inhibits GLUT1-mediated 3-O-methylglucose uptake in human erythrocytes [Vmax and Km for transport are reduced fourfold; Ki(app) = 3.5 mM Caffeine]. ATP and AMP antagonize Caffeine inhibition of 3-O-methylglucose uptake in erythrocyte ghosts by increasing Ki(app) for Caffeine inhibition of transport from 0.9 ± 0.3 mM in the absence of intracellular nucleotides to 2.6 ± 0.6 and 2.4 ± 0.5 mM in the presence of 5 mM intracellular ATP or AMP, respectively. Extracellular ATP has no effect on sugar uptake or its inhibition by Caffeine. Caffeine and ATP displace the fluorescent ATP derivative, trinitrophenyl-ATP, from the GLUT1 nucleotide-binding site, but d-glucose and the transport inhibitor cytochalasin B do not. Caffeine, but not ATP, inhibits cytochalasin B binding to GLUT1. Like ATP, Caffeine renders the GLUT1 carboxy-terminus less accessible to peptide-directed antibodies, but cytochalasin B and d-glucose do not.
These results suggest that the Caffeine-binding site bridges two nonoverlapping GLUT1 endofacial sites-the regulatory, nucleotide-binding site and the cytochalasin B-binding site. Caffeine binding to GLUT1 mimics the action of ATP but not cytochalasin B on sugar transport. Molecular docking studies support this hypothesis.
||The seeds of Theobroma cacao L.
||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Recent ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.PMID: 29328914
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.PMID: 29149595
Scientific Reports 2017 Dec 11;7(1):17332.doi: 10.1038/s41598-017-17427-6.PMID: 29230013
Molecules. 2017 Oct 27;22(11). pii: E1829.doi: 10.3390/molecules22111829.PMID: 29077044
J Cell Biochem. 2018 Feb;119(2):2231-2239.doi: 10.1002/jcb.26385. PMID: 28857247
Phytomedicine. 2018 Feb 1;40:37-47. doi: 10.1016/j.phymed.2017.12.030.PMID: 29496173
Calculate Dilution Ratios(Only for Reference)
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
|Reprod Biol. 2015 Mar;15(1):20-6. |
|Caffeine stimulates in vitro pituitary LH secretion in lipopolysaccharide-treated ewes.[Pubmed: 25726373]|
|The study was designed to determine the effects of Caffeine on luteinizing hormone (LH) secretion and gene expression of Caffeine-associated receptors in anterior pituitary (AP) explants obtained from saline- and lipopolysaccharide (LPS)-treated ewes.
METHODS AND RESULTS:
Animals had been treated with LPS or saline daily for seven days. Three hours after the last injection of LPS/saline, the AP were collected and divided into four explants. The explants were incubated with: 1/medium-199 (control explants), 2/gonadotropin-releasing hormone (GnRH; 100 pmol/mL; a positive control), 3/Caffeine (10 mmol/L), or 4/GnRH+Caffeine. Caffeine stimulated (p<0.05) LH release by explants from both saline (19.7 vs. control 12.6 ng/mg) and LPS (28.3 vs. control 13.9 ng/mg) treated animals. The effect of Caffeine on LH secretion was stronger in the LPS-treated group than in saline-treated group, and the observed LH release was similar to that induced by GnRH alone (27.2 ng/mg). Caffeine increased (p<0.05) LHβ gene expression only in explants from LPS-treated animals.
In conclusion, the results of the present study demonstrated a stimulatory in vitro effect of Caffeine on LH secretion by ovine pituitary explants. The potency of the Caffeine-induced LH secretion was affected by in vivo treatment of the animals with endotoxin.
Caffeine References Information
Neuroscience. 2015 Feb 11. pii: S0306-4522(15)00146-3.
|Anxiolytic-like, stimulant and neuroprotective effects of Ilex paraguariensis extracts in mice.[Pubmed: 25681522]|
|Overall, our results indicate the importance of the I. paraguariensis-induced CNS effects, since it is a widely used nutraceutical. We have reported anxiolytic, stimulant and neuroprotective effects for this plant species. These effects are potentially modulated by the cholinergic system as well as by Caffeine.