|Biological Activity or Inhibitors:||1. Coumarin 6(Coumarin VI) is a dye, it can monitor protein aggregation.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.8536 mL||14.2678 mL||28.5356 mL||57.0711 mL||71.3389 mL|
|5 mM||0.5707 mL||2.8536 mL||5.7071 mL||11.4142 mL||14.2678 mL|
|10 mM||0.2854 mL||1.4268 mL||2.8536 mL||5.7071 mL||7.1339 mL|
|50 mM||0.0571 mL||0.2854 mL||0.5707 mL||1.1414 mL||1.4268 mL|
|100 mM||0.0285 mL||0.1427 mL||0.2854 mL||0.5707 mL||0.7134 mL|
Analyst. 2011 May 21;136(10):2161-7.
|Coumarin 6 and 1,6-diphenyl-1,3,5-hexatriene (DPH) as fluorescent probes to monitor protein aggregation.[Pubmed: 21445407]|
|We report the use of Coumarin 6(Coumarin VI) and 1,6-diphenyl-1,3,5-hexatriene (DPH) for the identification of protein aggregates for the first time. The two dyes can be used at very low (nanomolar) concentrations and do not interfere with the aggregation process, as is reported for other commonly used fluorescent protein probes. In the presence of protein aggregates, their quantum yields are significantly high. DPH is able to recognize both amorphous and fibrillar aggregates but cannot distinguish between them. Coumarin 6(Coumarin VI) can distinguish between both types of aggregates. It also exhibits the characteristic sigmoidal curve of amyloid formation, with higher sensitivity for detection of fibrillation than the conventionally used Thioflavin T.|