| Structure Identification: |
| Pharmaceutical Biotechnology, 2013-04 | | Study on the distribution in cells by Ophiopogonin C.[Reference: WebLink] | To investigate the effect and its feature of location and accumulation of Ophiopogonin C(DT-13) in HUVEC/HeLa cells moreover, a new method for the study of efficacy and mechanism of saponins of Traditional Chinese medicine(TCM) is provided.
METHODS AND RESULTS:
We obtaind and purified enough DT-13 monoclonal antibodies with the pre-obtained DT-13 hybridoma cell line 1B12, its activities with icELISA are also detectad. The effect of proliferation on HUVEC/HeLa cells after DT-13 with different series of concentrations by MTT assay is investigated. The effect and its feature of location and accumulation of DT-13 in HUVEC/HeLa cells by immunflouresence(FITC & DAPI staining) method and studied. The results showed that under the no-cytotoxicitiy concentration of DT-13, it can be carried thraugh in to cells' cytoplasm quickly and then got into nucleus gradually and accumulated for a long time. When in HUVEC cells, DT-13 (5.0 μmol/L) is in cytoplasm 30 min before, 12 h later it is basically all into the nucleus accumulating for at least 24 h. While in HeLa cells, DT-13 (2.5 μmol/L) is faster to get into nucleus than in HUVEC cells, slao accumulate for at least 24 h.
CONCLUSIONS:
The resluts suggest that DT-13' pharmacological activities may be related to the substances in nucleus mainly, and DT-13' main mechanism remains to be further studied. In summary, DT-13' localization and dynamic process in cells can be detected by this method with the advantage of direct-viewing, reliability and convenience. Also, this method can be referred when studying on other saponins of TCM or their analogues. |
|
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)