|Source:||The herbs of Pueraria peduncularis|
|Biological Activity or Inhibitors:||1. Pedunsaponin A , a novel molluscicidal compound, exhibits strong toxicity against Pomacea canaliculata.
2. Pedunsaponin A has significant toxic effects on different organs of the snail.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.233 mL||6.1652 mL||12.3305 mL||24.6609 mL||30.8261 mL|
|5 mM||0.2466 mL||1.233 mL||2.4661 mL||4.9322 mL||6.1652 mL|
|10 mM||0.1233 mL||0.6165 mL||1.233 mL||2.4661 mL||3.0826 mL|
|50 mM||0.0247 mL||0.1233 mL||0.2466 mL||0.4932 mL||0.6165 mL|
|100 mM||0.0123 mL||0.0617 mL||0.1233 mL||0.2466 mL||0.3083 mL|
Pestic Biochem Physiol. 2018 Jun;148:151-158.
|Histopathological effects of Pedunsaponin A on Pomacea canaliculata.[Pubmed: 29891366 ]|
|Pedunsaponin A, a novel molluscicidal compound isolated from Pueraria peduncularis, exhibits strong toxicity against Pomacea canaliculata. To determine the mechanisms of Pedunsaponin A toxicity, its effects on the organs and hemocytes of P. canaliculata were examined in this study. The results showed that Pedunsaponin A had significant toxic effects on different organs of the snail, including the lungs, gills, mantle, siphon tube, ventricle, pericardial cavity, hepatopancreas, kidneys, and the major symptom of this toxicity was the loss of cilia in the lungs and gills. Additionally, in further studies on the effects of Pedunsaponin A treatment, we found that the hemocyte count was changed and hemocyte morphology was damaged, which was primarily reflected by cytoplasm leakage, nuclei deformation, and significant reductions in the number of ribosomes and granulocyte mitochondria. Based on these results and considering that blood vessels are distributed in the lungs and gills, we hypothesized that Pedunsaponin A would first destroy the cilia, which disrupt physiological activities such as respiration, excretion and feeding, and then enter the hemolymph through blood vessels, disrupt the normal function of the hemocytes and destroy the snail immune system, eventually resulting in the death of the snail.|
Pest Manag Sci. 2017 Jun;73(6):1143-1147.
|Active saponins from root of Pueraria peduncularis (Grah. ex Benth.) Benth. and their molluscicidal effects on Pomacea canaliculata.[Pubmed: 27608163 ]|
|ACKGROUND: Pueraria peduncularis (Grah. ex Benth.) Benth., which belongs to the Leguminosae family, exhibits resistance to many crop pests in agricultural production. Pomacea canaliculata is an important invasive snail in rice fields and causes severe yield losses. To evaluate the toxicity of P. peduncularis to P. canaliculata, in this study the molluscicidal activity of root extracts of P. peduncularis was tested against P. canaliculata; the active compounds were isolated, and the structures of these compounds were analysed using nuclear magnetic resonance (NMR) analysis and mass spectral analysis. RESULTS: Our results showed that the molluscicidal activity of the root crude extract differed between P. canaliculata with different shell diameters after treatment for 72 h. The median lethal concentration (LC50 ) was 5.511 mg L-1 against snails of 1.5 ± 0.2 cm diameter and 12.383 mg L-1 against snails of 2.5 ± 0.2 cm diameter. Furthermore, two active ingredients isolated from root methanol extracts were identified as Pedunsaponin A and pedunsaponin C. Both Pedunsaponin A and pedunsaponin C showed strong molluscicidal activities, with LC50 values of 3.893 and 4.252 mg L-1 , respectively, against snails with shell diameters of 1.5 ± 0.2 cm after treatment for 72 h. CONCLUSION: Pueraria peduncularis extracts exhibit high molluscicidal activity and have great potential value for exploring a molluscicide to control Pomacea canaliculata.|