|Source:||The bark of Phellodendron chinense|
|Biological Activity or Inhibitors:||1. Piscidinol A is toxic against the 4T1 and HEp2 cancer cell lines, the IC50 of 8.0 ± 0.03 and 8.4 ± 0.01 uM, respectively.
2. Piscidinol A can inhibit NO production in mouse peritoneal macrophages with inhibitory ratios ranging from 39.8±7.7 to 68.2±4.5%.
|Solvent:||Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.1066 mL||10.533 mL||21.0659 mL||42.1319 mL||52.6648 mL|
|5 mM||0.4213 mL||2.1066 mL||4.2132 mL||8.4264 mL||10.533 mL|
|10 mM||0.2107 mL||1.0533 mL||2.1066 mL||4.2132 mL||5.2665 mL|
|50 mM||0.0421 mL||0.2107 mL||0.4213 mL||0.8426 mL||1.0533 mL|
|100 mM||0.0211 mL||0.1053 mL||0.2107 mL||0.4213 mL||0.5266 mL|
Chem Biodivers. 2013 Apr;10(4):695-702.
|Tirucallane triterpenoids from the stems of Brucea mollis.[Pubmed: 23576355]|
|Three new tirucallane triterpenoids, brumollisols A-C (1-3, resp.), together with five known analogues, (23R,24S)-23,24,25-trihydroxytirucall-7-ene-3,6-dione (4), Piscidinol A (5), 24-epiPiscidinol A (6), 21α-methylmelianodiol (7), and 21β-methylmelianodiol (8), were isolated from an EtOH extract of the stems of Brucea mollis. Their structures were elucidated by means of spectroscopic methods including 1D- and 2D-NMR techniques and mass spectrometry. In the in vitro assays, compound 6 exhibited significant cytotoxic activity against A549 and BGC-823 cancer cells with IC50 values of 1.16 and 3.01 μM, respectively. At a concentration of 10 μM, compounds 1-5, 7, and 8 were found to inhibit NO production in mouse peritoneal macrophages with inhibitory ratios ranging from 39.8±7.7 to 68.2±4.5%.|
J Ethnopharmacol. 2015 Nov 4;174:419-25.
|Antiplasmodial and cytotoxic activities of the constituents of Turraea robusta and Turraea nilotica.[Pubmed: 26320684 ]|
|The extracts showed good to moderate antiplasmodial activities, where the extract of the stem bark of T. robusta was also cytotoxic against the 4T1 and the HEp2 cells (IC50<10 μg/ml). The compounds isolated from these extracts were characterized as limonoids, protolimonoids and phytosterol glucosides. These compounds showed good to moderate activities with the most active one being azadironolide, IC50 2.4 ± 0.03 μM and 1.1 ± 0.01 μM against the D6 and W2 strains of Plasmodium falciparum, respectively; all other compounds possessed IC50 14.4-40.5 μM. None of the compounds showed significant cytotoxicity against vero cells, yet four of them were toxic against the 4T1 and HEp2 cancer cell lines with Piscidinol A having IC50 8.0 ± 0.03 and 8.4 ± 0.01 μM against the 4T1 and HEp2 cells, respectively. Diacetylation of Piscidinol A resulted in reduced cytotoxicity.|