|Source:||The rhizomes of Polygonum cuspidatum Sieb. et Zucc.|
|Biological Activity or Inhibitors:||1. Polydatin is a mitochondria protector for acute severe hemorrhagic shock treatment, the neuronal mitochondrial injury is involved in the genesis of severe shock and polydatin may be the best choice for protection of neuron against mitochondrial injury in severe shock.
2. Polydatin has favorable potency to develop a hypolipemic and/or hepatoprotective agent in clinic.
3. Polydatin has a protective effect against ischemia/reperfusion injury in rat heart, the cardioprotection of polydatin is mainly mediated by cNOS which leading to an increase in NO production.
4. Polydatin is a natural precursor of resveratrol, it can induce β-defensin production and reduce inflammatory response, they reinforce cytoprotective response in stress conditions and suggest their use in cosmetic or pharmaceutical preparations.
5. Polydatin modulates inflammation by decreasing NF-κB activation and oxidative stress by increasing Gli1, Ptch1, SOD1 expression and ameliorates blood-brain barrier permeability for its neuroprotective effect in pMCAO rat brain.
6. Polydatin has antioxidant activity.
7. Polydatin inhibits growth of lung cancer cells by inducing apoptosis and causing cell cycle arrest.
|Solvent:||DMSO, Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.5615 mL||12.8074 mL||25.6148 mL||51.2295 mL||64.0369 mL|
|5 mM||0.5123 mL||2.5615 mL||5.123 mL||10.2459 mL||12.8074 mL|
|10 mM||0.2561 mL||1.2807 mL||2.5615 mL||5.123 mL||6.4037 mL|
|50 mM||0.0512 mL||0.2561 mL||0.5123 mL||1.0246 mL||1.2807 mL|
|100 mM||0.0256 mL||0.1281 mL||0.2561 mL||0.5123 mL||0.6404 mL|
Inflammation. 2015 Jan 8.
|Inhibitory Effects of Polydatin on Lipopolysaccharide-Stimulated RAW 264.7 Cells.[Pubmed: 25567371]|
|The results revealed that Polydatin effectively inhibited nitric oxide and prostaglandin E2 production, and it is not surprising that Polydatin reduced inducible nitric oxide synthase and cyclooxygenase-2 expression at protein and transcriptional levels. Additionally, Polydatin significantly ameliorated the activation of NF-κB and the phosphorylation of MAPKs in LPS-induced RAW 264.7 macrophages. These findings suggested that Polydatin exerted potent anti-inflammatory activity in macrophages.|
Zhongguo Zhong Yao Za Zhi. 2014 Aug;39(16):3157-61.
|Inhibitory effect of polydatin on expression of toll-like receptor 4 in ischemia-reperfusion injured NRK-52E cells.[Pubmed: 25509306]|
|Polydatin downregulated I/R induced mRNA and protein expressions of TLR4, and decreased the protein expression of NF-κB, TNF-α and IL-1β. The TLR4 blocker partially antagonized the effect of I/R on NF-κB signaling, and such inhibitory effect was markedly enhanced by Polydatin. In the present study, Polydatin protects NRK-52E cells from I/R injury possibly by relieving the inflammatory response through regulation of TLR4/NF-κB signaling pathway.|
Eur J Pharmacol. 2014 Dec 15;745:152-65.
|Polydatin improves glucose and lipid metabolism in experimental diabetes through activating the Akt signaling pathway.[Pubmed: 25310908]|
|The results indicate that Polydatin regulates glucose and lipid metabolism in experimental diabetic models, the underlying mechanism is probably associated with regulating the Akt pathway. The effect of Polydatin on increased Akt phosphorylation is independent of prompting insulin secretion, but dependent of increasing IRS phosphorylation.|
Oncol Lett. 2014 Jan;7(1):295-301. Epub 2013 Nov 21.
|Polydatin inhibits growth of lung cancer cells by inducing apoptosis and causing cell cycle arrest.[Pubmed: 24348867]|
|Polydatin (PD), a small natural compound from Polygonum cuspidatum, has a number of biological functions. However, the anticancer activity of Polydatin has been poorly investigated. In the present study, thiazolyl blue tetrazolium bromide assay was used to evaluate the inhibitory effect of Polydatin on cell growth. Cell cycle distribution and apoptosis were investigated by flow cytometry. In addition, the expression of several proteins associated with apoptosis and cell cycle were analyzed by western blot analysis. The results demonstrated that Polydatin significantly inhibits the proliferation of A549 and NCI-H1975 lung cancer cell lines and causes dose-dependent apoptosis. Cell cycle analysis revealed that Polydatin induces S phase cell cycle arrest. Western blot analysis showed that the expression of Bcl-2 decreased as that of Bax increased, and the expression of cyclin D1 was also suppressed. The results suggest that Polydatin has potential therapeutic applications in the treatment of lung cancer.|
Expert Opin Investig Drugs. 2013 Feb;22(2):169-79.
|Polydatin--a new mitochondria protector for acute severe hemorrhagic shock treatment.[Pubmed: 23241098 ]|
|Increased lipid peroxides (LPO) levels, lysosomal injury and mitochondrial permeability transition pore opening took place in neurons, resulting in swollen mitochondria with poorly defined cristae, decreased mitochondrial membrane potential (ΔΨ) and reduced ATP content in shock + NS group, indicating mitochondrial dysfunction. Mitochondrial protectors, such as CsA, Res and Polydatin, partially inhibited these alterations, especially following Polydatin protection, ATP level increased from 44.14 ± 13.81% in shock + NS group to 89.57 ± 9.21% and the survival time was prolonged from 6.3 ± 5.9 h in the shock + NS group to 31.6 ± 13.7 h in shock + Polydatin group. CONCLUSIONS: The study shows that neuronal mitochondrial injury is involved in the genesis of severe shock and Polydatin may be the best choice for protection of neuron against mitochondrial injury in severe shock.|
Biomed Pharmacother. 2009 Aug;63(7):457-62.
|Effects of polydatin from Polygonum cuspidatum on lipid profile in hyperlipidemic rabbits.[Pubmed: 18657948]|
|The results showed that Polydatin markedly decreased the serum levels of total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) in hyperlipidemic rabbits. The ratio of TC to high-density lipoprotein cholesterol (HDL-C) and the liver coefficient were also reduced. But both Polydatin and high fat/cholesterol diet did not evidently affect body weight in hyperlipidemic rabbits. All these results suggest that Polydatin from Polygonum cuspidatum has favorable potency to develop a hypolipemic and/or hepatoprotective agent in clinic. However the mechanism of hypolipemic action of Polydatin is in need of further clarity.|
Sheng Li Xue Bao. 2008 Apr 25;60(2):161-8.
|Protective effect of polydatin against ischemia/reperfusion injury in rat heart.[Pubmed: 18425301]|
|The results showed: (1) Arrhythmia score and myocardial infarct size were significantly lower in Polydatin group than that in the control group (P<0.05, P<0.01); (2) The recovery of LVDP, ±LVdp/dt(max) and CF during reperfusion in Polydatin group were significantly better than that in the control rats (P<0.05, P<0.01); (3) SOD activity in Polydatin group was significantly higher than that in the control group, but MDA content was lower in Polydatin group than that in the control group (P<0.05); (4) NO content and NOS activity, especially constitutive nitric oxide synthase (cNOS) activity in Polydatin group were higher than that in the control group (P<0.05); (5) L-NAME, the NOS inhibitor, reversed the protective effect of Polydatin against ischemia/reperfusion injury. The results suggest that Polydatin has a protective effect against ischemia/reperfusion injury in rat heart. The cardioprotection of Polydatin is mainly mediated by cNOS which leading to an increase in NO production.|
Brain Res Bull. 2012 Jan 4;87(1):50-9.
|Polydatin modulates inflammation by decreasing NF-κB activation and oxidative stress by increasing Gli1, Ptch1, SOD1 expression and ameliorates blood-brain barrier permeability for its neuroprotective effect in pMCAO rat brain.[Pubmed: 22001340 ]|
|Inflammation and oxidative stress play an important role in cerebral ischemic pathogenesis. Polydatin has been proved to elicit numerous biological effects through its anti-inflammatory and anti-oxidant properties. However, little is known regard to the mechanism of Polydatin's neuroprotection in ischemic stroke. We therefore investigated the potential neuroprotective effects of Polydatin and explored the underlying mechanisms. The results indicated that Polydatin protected the brain from damage caused by pMCAO, and this effect may be through up-regulating the expression of Gli1, Ptch1 and SOD1 and down-regulating the expression of NF-κB, and ameliorating BBB permeability.|