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    CAS No. 54328-09-5 Price $318 / 10mg
    Catalog No.CFN90677Purity> 98%
    Molecular Weight522.46Type of CompoundAnthraquinones
    FormulaC30H18O9Physical DescriptionYellow powder
    Download     COA    MSDS    SDFSimilar structuralComparison (Web)
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    Protopseudohypericin Description
    Source: The herbs of Hypericum perforatum L.
    Biological Activity or Inhibitors:
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.914 mL 9.5701 mL 19.1402 mL 38.2804 mL 47.8506 mL
    5 mM 0.3828 mL 1.914 mL 3.828 mL 7.6561 mL 9.5701 mL
    10 mM 0.1914 mL 0.957 mL 1.914 mL 3.828 mL 4.7851 mL
    50 mM 0.0383 mL 0.1914 mL 0.3828 mL 0.7656 mL 0.957 mL
    100 mM 0.0191 mL 0.0957 mL 0.1914 mL 0.3828 mL 0.4785 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protopseudohypericin References Information
    Citation [1]

    Monatshefte für Chemie / Chemical Monthly August/September 1992, Volume 123, Issue 8-9, pp 731-739

    On the nature of “soluble” hypericin inHypericum species[Reference: WebLink]
    Using NMR experiments it was deduced that hypericin, pseudohypericin, protohypericin, and Protopseudohypericin are present in the plant as their rapidly interconverting 3- and 4-phenolate ions. From AAS the main counter-ion of these phenolates was derived to be potassium. The potassium and N-ethyl-N,N-diisopropylammonium salts of hypericin were synthesizcd for comparison. A preparative procedure to isolate hypericin and pseudohypericin from plant material was developed.
    Citation [2]

    Phytochemical Analysis Volume 18, Issue 3, pages 204–208, May/June 2007

    Simultaneous determination of total hypericin and hyperforin in St. John's wort extracts by HPLC with electrochemical detection[Reference: WebLink]
    An analytical procedure was developed for the simultaneous determination of total hypericin (Protopseudohypericin, pseudohypericin, protohypericin and hypericin) and hyperforin in Hypericum perforatum (St. John's wort) extracts and its preparations. The determination of total hypericin and hyperforin in one step was achieved by exposing the samples to artificial daylight in amber glass vials. This procedure allows both the photoconversion of the protoforms into the appropriate hypericins and the protection of the photosensitive hyperforin. For quantification, an HPLC method with electrochemical detection was applied. As an example of the application of the principle, two preparations containing St. John's wort were assayed.