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Pulsatilla saponin H
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Pulsatilla saponin H
Price:
CAS No.: 68027-14-5
Catalog No.: CFN80187
Molecular Formula: C65H106O31
Molecular Weight: 1382.67 g/mol
Purity: >=98%
Type of Compound: Triterpenoids
Physical Desc.: Powder
Source: The herbs of Pulsatilla chinensis
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Download: COA    MSDS
Similar structural: Comparison (Web)  (SDF)
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Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
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Description: Reference standards.
Pulsatilla saponin H Description
Source: The herbs of Pulsatilla chinensis
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

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doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 0.7232 mL 3.6162 mL 7.2324 mL 14.4648 mL 18.081 mL
5 mM 0.1446 mL 0.7232 mL 1.4465 mL 2.893 mL 3.6162 mL
10 mM 0.0723 mL 0.3616 mL 0.7232 mL 1.4465 mL 1.8081 mL
50 mM 0.0145 mL 0.0723 mL 0.1446 mL 0.2893 mL 0.3616 mL
100 mM 0.0072 mL 0.0362 mL 0.0723 mL 0.1446 mL 0.1808 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Structure Identification:
Phytochemical Analysis, 2010, 21(4):314-321.
Quality Control of Pulsatilla koreana Based on the Simultaneous Determination of Triterpenoidal Saponins by HPLC-ELSD and Principal Component Analysis[Reference: WebLink]
Pulsatilla koreana Nakai, with triterpenoidal saponins as its main pharmacological effective compounds, is known to have several biological activities, including hypoglycaemic, antitumour, cognition-enhancing, neuroprotective, cytotoxic and antiangiogenic activities. However, few analytical methods have been reported on the quality assessment of P. koreana roots.
METHODS AND RESULTS:
To establish a high-performance liquid chromatography coupled with evaporative light scattering detection for the simultaneous determination of five triterpenoidal saponins, including pulsatilloside E (1), Pulsatilla saponin H (2), anemoside B4 (3), hederacolchiside E (4) and cussosaponin C (5) in P. koreana. The chromatographic separation was performed on a Shiseido CapCell PAK C18 analytical column efficiently using gradient elution with acetonitrile and water. All calibration curves showed excellent linear regressions (R(2) > 0.9996) within the range of tested concentrations. The intra- and inter-day variations were below 4.78% in terms of RSD. The recoveries were 94.82-102.97% with RSD of 0.27-3.92% for spiked P. koreana samples.
CONCLUSIONS:
The validated method was successfully used for the analysis of five saponins in P. koreana from different locations. Moreover, the different samples were clustered in accordance with contents of triterpenoidal saponins based on aglycon type by a principal component analysis.
Cyanidin-3-O-arabinoside chloride

Catalog No: CFN92041
CAS No: 111613-04-8
Price: $318/10mg
Sagittatoside B

Catalog No: CFN90211
CAS No: 118525-36-3
Price: $298/10mg
Peimine

Catalog No: CFN99996
CAS No: 23496-41-5
Price: $50/20mg
Sinapic acid

Catalog No: CFN92631
CAS No: 530-59-6
Price: $30/20mg
Moracin D

Catalog No: CFN97179
CAS No: 69120-07-6
Price: $ /
Isoastragaloside I

Catalog No: CFN90977
CAS No: 84676-88-0
Price: $268/10mg
Astragaloside III

Catalog No: CFN99174
CAS No: 84687-42-3
Price: $268/20mg
Kaempferol-7-O-D-glucopyranoside

Catalog No: CFN90150
CAS No: 16290-07-6
Price: $268/5mg
Forsythoside A

Catalog No: CFN99705
CAS No: 79916-77-1
Price: $50/20mg
Jervine

Catalog No: CFN90955
CAS No: 469-59-0
Price: $40/20mg
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