| In vitro: |
| 《Chinese Journal of Natural Medicines》 2008-04 | | Interactions of Fufang Danshen Prescription with Liposome Biomembrane[Reference: WebLink] | To study the interactions between Fufang Danshen Prescription (FDP) and liposome biomembrane.
METHODS AND RESULTS:
Based on liposome equilibrium dialysis system, the constituents having interaction with liposome (as the model of biomembrane) in FDP were analyzed and identified by high performance liquid chromatography (HPLC) and time-of-flight mass spectrometry (TOF-MS) analysis. And the effect of pH value was also investigated on their interactions. Twenty-four constitutes in FDP interact with liposome obviously. Among them, eighteen compounds were identified in comparison with the standards in terms of UV spectrum, MS data along with retention time, including seven phenolic acids (danshensu, protocatechuic aldehyde, salvianolic acid E, rosmarinic acid, lithospermic acid, salvianolic acid B and salvianolic acid A), six diterpenoid qui-nines (dihydrotanshinone I, Tetrahydro tanshinone I, cryptotanshinone, tanshinone I, methylene tanshiqunone and tanshinone IIA) and five saponins (notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1, ginsenoside Rd and notoginsenoside K).
CONCLUSIONS:
Liposome equilibrium dialysis system coupled with HPLC and LC/TOF/MS could simply and effectively esti-mate the absorption of multiple components in FDP in vivo, and predicts the potential bioactivity of these components, and thus contributes to the investigation of bioactive compounds with curative effect in FDP. | | Phytother Res . 2015 Feb;29(2):281-7. | | Soyasaponins Aa and Ab exert an anti-obesity effect in 3T3-L1 adipocytes through downregulation of PPARγ[Pubmed: 25366162] | | Abstract
Saponins are a diverse group of biologically functional products in plants. Soyasaponins are usually glycosylated, which give rise to a wide diversity of structures and functions. In this study, we investigated the effects and molecular mechanism of soyasaponins Aa and Ab in regulating adipocyte differentiation and expression of adipogenic marker genes in 3T3-L1 adipocytes. Soyasaponins Aa and Ab dose-dependently inhibited the accumulation of lipids and the expression of adiponectin, adipocyte determination and differentiation factor 1/sterol regulatory element binding protein 1c, adipocyte fatty acid-binding protein 2, fatty acid synthase, and resistin in 3T3-L1 adipocytes. In addition, soyasaponins Aa and Ab suppressed the transcriptional activity of peroxisome proliferator-activated receptor γ (PPARγ) in HEK 293T cells. Furthermore, we confirmed that the expression of PPARγ and of CCAAT-enhancer-binding protein α (C/EBPα) was suppressed at both the mRNA and protein levels in 3T3-L1 adipocytes by treatment with soyasaponins Aa and Ab. Taken together, these findings indicate that soyasaponin Aa and Ab markedly inhibit adipocyte differentiation and expression of various adipogenic marker genes through the downregulation of the adipogenesis-related transcription factors PPARγ and C/EBPα in 3T3-L1 adipocytes.
Keywords: 3T3-L1 cells; adipogenesis; adipogenic marker genes; peroxisome proliferator-activated receptor gamma (PPARγ); soyasaponins. |
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