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Vitamin D3
Vitamin D3
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Vitamin D3
Price: $40 / 20mg
CAS No.: 67-97-0
Catalog No.: CFN90027
Molecular Formula: C27H44O
Molecular Weight: 384.64 g/mol
Purity: >=98%
Type of Compound: Miscellaneous
Physical Desc.: White powder
Source:
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
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Similar structural: Comparison (Web)  (SDF)
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According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $10.8 / In-stock
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Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Vitamin D3 is a form of vitamin D, binds and activates a H305F/H397Y mutant vitamin D receptor (VDR) with EC50 of 300 nM. Supplemental calcium and Vitamin D3 may increase TGFβ1 expression and shift TGFα expression downward from the differentiation to the proliferation zone in the crypts in the normal-appearing colorectal mucosa of sporadic colorectal adenoma patients.
Targets: TGF-β/Smad
In vitro:
J Pharm Biomed Anal. 2014 Mar;91:73-80.
Development of a validated UPLC method for simultaneous estimation of both free and entrapped (in solid lipid nanoparticles) all-trans retinoic acid and cholecalciferol (vitamin D3) and its pharmacokinetic applicability in rats.[Pubmed: 24440824]
A sensitive ultra-performance liquid chromatography (UPLC) method was developed for simultaneous estimation of all-trans retinoic acid (ATRA) and cholecalciferol (Vitamin D3) in rat plasma.
METHODS AND RESULTS:
The method was validated over the linear range of 1.0-5000ng/ml (r(2)=0.999) for both vitamins with a limit of detection of 0.5ng/ml. Chromatographic separation was achieved using liquid-liquid extraction (LLE) on an Acquity BEH RP 18 column (2.1mm×50mm, I.D. 1.7μm), with mobile phase comprising of acetonitrile:methanol:water (90:8:2, v/v/v), at a flow rate of 0.20ml/min and a total run time of 5min. Intra and inter-day variability (RSD) was ≤3.1%, and the accuracy varied between 95.4-99.9% and 95.3-101.1% respectively, for ATRA and 98.5-100.8% and 99.3-101.7%, respectively for Vitamin D3. High recovery of ≥96.0% for ATRA and ≥87.80% for Vitamin D3 was achieved. ATRA and Vitamin D3 were stable in plasma under different storage and processing conditions. The method was applied to estimate the total drug content and entrapment efficiency of ATRA and Vitamin D3 loaded solid lipid nanoparticles (SLNs). Concentration of these two agents was determined in rat plasma after simultaneous subcutaneous administration in free form or when loaded into SLNs thus establishing pharmacokinetic application of the developed procedure.
CONCLUSIONS:
Results indicated an improvement in AUC0-∞ by 5.4 times and 29.4 times for ATRA and Vitamin D3, respectively, upon their incorporation into SLNs. Simultaneous administration of these two vitamins and their improved and prolonged bioavailability has scope for their use in treatment and control of tuberculosis.
In vivo:
Eur J Endocrinol. 2015 Mar;172(3):235-41.
Vitamin D3 increases in abdominal subcutaneous fat tissue after supplementation with vitamin D3.[Pubmed: 25661743]
The objective was to assess the amount of Vitamin D3 stored in adipose tissue after long-term supplementation with high dose Vitamin D3. A cross-sectional study on 29 subjects with impaired glucose tolerance who had participated in a randomized controlled trial with Vitamin D3 20 000 IU (500 μg) per week vs placebo for 3-5 years.
METHODS AND RESULTS:
Abdominal subcutaneous fat tissue was obtained by needle biopsy for the measurements of Vitamin D3 and 25-hydroxyVitamin D3 (25(OH)D3). Body fat was measured with dual-energy X-ray absorptiometry, and serum 25(OH)D3 level was quantified. In the subjects given Vitamin D3, the median concentrations of serum 25(OH)D3, fat Vitamin D3, and fat 25(OH)D3 were 99 nmol/l, 209 ng/g, and 3.8 ng/g, respectively; and correspondingly in the placebo group 62  nmol/l, 32 ng/g, and 2.5 ng/g. If assuming an equal amount of Vitamin D3 stored in all adipose tissue in the body, the median body store was 6.6 mg Vitamin D3 and 0.12 mg 25(OH)D3 in those given Vitamin D3.
CONCLUSIONS:
Subcutaneous adipose tissue may store large amounts of Vitamin D3. The clinical importance of this storage needs to be determined.
J Steroid Biochem Mol Biol. 2015 Apr;148:275-82.
Dissecting high from low responders in a vitamin D3 intervention study.[Pubmed: 25448738]
Vitamin D3 is a pleiotropic signaling molecule that has via activation of the transcription factor vitamin D receptor (VDR) a direct effect on the expression of more than 100 genes. The aim of this study was to find transcriptomic and clinical biomarkers that are most suited to identify Vitamin D3 responders within 71 pre-diabetic subjects during a 5-month intervention study (VitDmet).
METHODS AND RESULTS:
In hematopoietic cells, the genes ASAP2, CAMP, CD14, CD97, DUSP10, G0S2, IL8, LRRC8A, NINJ1, NRIP1, SLC37A2 and THBD are known as primary vitamin D targets. We demonstrate that each of these 12 genes carries a conserved VDR binding site within its genomic region and is expressed in human peripheral blood mononuclear cells (PBMCs). The changes in the expression of these genes in human PBMCs at the start and the end of the vitamin D-intervention were systematically correlated with the alteration in the circulating form of Vitamin D3, 25-hydroxyVitamin D3 (25(OH)D3). Only 39-44 (55-62%) of the study subjects showed a highly significant response to Vitamin D3, i.e., we considered them as "responders". In comparison, we found for 37-53 (52-75%) of the participants that only 12 biochemical and clinical parameters, such as concentrations of parathyroid hormone (PTH) and insulin, or computed values, such as homeostatic model assessment and insulin sensitivity index, show a correlation with serum 25(OH)D3 levels that is as high as that of the selected VDR target genes. All 24 parameters together described the pleiotropic vitamin D response of the VitDmet study subjects. Interestingly, they demonstrated a number of additional correlations that define a network, in which PTH plays the central role.
CONCLUSIONS:
In conclusion, Vitamin D3-induced changes in human PBMCs can be described by transcriptomic and serum biomarkers and allow a segregation into high and low responders. This article is part of a Special Issue entitled '17th Vitamin D Workshop' .
Vitamin D3 Description
Source:
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

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After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.5998 mL 12.9992 mL 25.9983 mL 51.9967 mL 64.9958 mL
5 mM 0.52 mL 2.5998 mL 5.1997 mL 10.3993 mL 12.9992 mL
10 mM 0.26 mL 1.2999 mL 2.5998 mL 5.1997 mL 6.4996 mL
50 mM 0.052 mL 0.26 mL 0.52 mL 1.0399 mL 1.2999 mL
100 mM 0.026 mL 0.13 mL 0.26 mL 0.52 mL 0.65 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Kinase Assay:
Mol Carcinog. 2015 Apr;54(4):270-80.
Effects of calcium and vitamin D3 on transforming growth factors in rectal mucosa of sporadic colorectal adenoma patients: a randomized controlled trial.[Pubmed: 24166893]
Transforming growth factor alpha (TGFα) and TGFβ1 are growth-promoting and -inhibiting autocrine/paracrine growth factors, respectively, that may (1) affect risk for colorectal cancer and (2) be modifiable by anti-proliferative exposures. The effects of supplemental calcium and Vitamin D3 on these two markers in the normal-appearing colorectal mucosa in humans are unknown.
METHODS AND RESULTS:
We conducted a pilot, randomized, double-blind, placebo-controlled, 2 × 2 factorial clinical trial (n = 92; 23/treatment group) of calcium 2 g and/or Vitamin D3 800 IU/d versus placebo over 6 mo. TGFα and TGFβ1 expression was measured in biopsies of normal-appearing rectal mucosa using automated immunohistochemistry and quantitative image analysis at baseline and 6-mo follow-up. In the calcium, Vitamin D3 , and calcium plus Vitamin D3 groups relative to the placebo group (1) the mean overall expression of TGFβ1 increased by 14% (P= 0.25), 19% (P = 0.17), and 22% (P = 0.09); (2) the ratio of TGFα expression in the upper 40% (differentiation zone) to that in the lower 60 (proliferation zone) of the crypts decreased by 34% (P = 0.11), 31% (P = 0.22), and 26% (P = 0.33); and (3) the TGFα/TGFβ1 ratio in the upper 40% of the crypts decreased by 28% (P = 0.09), 14% (P = 0.41), and 22% (P = 0.24), respectively.
CONCLUSIONS:
These preliminary results, although not statistically significant, suggest that supplemental calcium and Vitamin D3 may increase TGFβ1 expression and shift TGFα expression downward from the differentiation to the proliferation zone in the crypts in the normal-appearing colorectal mucosa of sporadic colorectal adenoma patients, and support further investigation in a larger clinical trial.
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