|Description:||1. Alpha-Spinasterol has anti-inflammatory activity.|
2. Alpha-Spinasterol is a novel efficacious and safe antagonist of the TRPV1 receptor with antinociceptive effect.
3. Alpha-Spinasterol can prevent TP-induced prostatic hyperplasia and may be beneficial in the management of benign prostatic hyperplasia.
4. Alpha-Spinasterol is a potent inhibitor (IC(50)= 3.9 x 10 (-12) g/mL, 9.5 pmol/L) of glomerular mesangial cell proliferation caused by high-ambient glucose (5.6 mM vs. 25 mM), it has a significant therapeutic potential to modulate the development and/or progression of diabetic nephropathy.
|Targets:||TRPV | HMG-CoA reductase|
|Source:||The roots of Bupleurum chinense DC.|
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.4231 mL||12.1153 mL||24.2307 mL||48.4614 mL||60.5767 mL|
|5 mM||0.4846 mL||2.4231 mL||4.8461 mL||9.6923 mL||12.1153 mL|
|10 mM||0.2423 mL||1.2115 mL||2.4231 mL||4.8461 mL||6.0577 mL|
|50 mM||0.0485 mL||0.2423 mL||0.4846 mL||0.9692 mL||1.2115 mL|
|100 mM||0.0242 mL||0.1212 mL||0.2423 mL||0.4846 mL||0.6058 mL|
J Neural Transm. 2015 Mar 13.
|α-Spinasterol, a TRPV1 receptor antagonist, elevates the seizure threshold in three acute seizure tests in mice.[Pubmed: 25764210]|
|The aim of the present study was to investigate the effect of alpha-Spinasterol on the seizure threshold in three acute models of seizures, i.e., in the intravenous (i.v.) pentylenetetrazole (PTZ) seizure test, in the maximal electroshock seizure threshold (MEST) test and in the model of psychomotor seizures induced by 6 Hz stimulation in mice. Our results revealed significant anticonvulsant effect of alpha-Spinasterol in all the used seizure tests. In the i.v. PTZ test, statistically significant elevation was noted in case of the threshold for myoclonic twitches (doses of 0.1-1 mg/kg) and generalized clonus seizures (doses of 0.5 and 1 mg/kg) but not for tonic seizures. The studied TRPV1 antagonist also increased the threshold for tonic hindlimb extension in the MEST (doses of 0.5 and 1 mg/kg) and 6 Hz psychomotor seizure (doses of 0.1 and 0.5 mg/kg) tests in mice. Furthermore, alpha-Spinasterol did not produce any significant impairment of motor coordination (assessed in the chimney test) and muscular strength (investigated in the grip-strength test) and it did not provoke significant changes in body temperature in mice. Based on the results of our study and the fact that alpha-Spinasterol is characterized by good blood-brain permeability, we postulate further investigation of this compound to precisely evaluate mechanism of its anticonvulsant action and opportunity of its usage in clinical practice.|
Mol Med Rep. 2014 Jun;9(6):2362-6.
|α-Spinasterol from Melandrium firmum attenuates benign prostatic hyperplasia in a rat model.[Pubmed: 24682042]|
|The present study investigated whether alpha-Spinasterol isolated from Melandrium firmum Rohrbach could prevent benign prostatic hyperplasia (BPH) induced by testosterone propionate (TP) in rats. The TP-induced increase was significantly inhibited in alpha-Spinasterol-treated rats when compared with the negative controls (P<0.05). In addition, histopathological examination demonstrated that alpha-Spinasterol treatment suppressed TP-induced prostatic hyperplasia. It is concluded that alpha-Spinasterol can prevent TP-induced prostatic hyperplasia and may be beneficial in the management of BPH.|
J Ethnopharmacol. 2014;151(1):144-50.
|Anti-inflammatory action of hydroalcoholic extract, dichloromethane fraction and steroid α-spinasterol from Polygala sabulosa in LPS-induced peritonitis in mice.[Pubmed: 24161429]|
|This study was designed to investigate the anti-inflammatory properties of the hydroalcoholic extract (HEPs), CH2Cl2 fraction and the steroid alpha-Spinasterol obtained from the aerial parts of Polygala sabulosa in a model of acute inflammation induced by intraperitoneal injection of bacterial lipopolysaccharide in mice. CONCLUSIONS: Taken together, these results provide the first experimental evidence demonstrating that HEPs have significant anti-inflammatory effects on LPS-induced inflammation. These effects appear to be, at least in part, due to the presence of alpha-Spinasterol. These findings support the widespread use of Polygala sabulosa in popular medicine and demonstrate that this plant has therapeutic potential for the development of phytomedicines with anti-inflammatory properties.|
J Pharmacol Exp Ther. 2012 Nov;343(2):258-69.
|Identification of the plant steroid α-spinasterol as a novel transient receptor potential vanilloid 1 antagonist with antinociceptive properties.[Pubmed: 22837009]|
|Among the compounds isolated from the dichloromethane fraction, only alpha-Spinasterol reduced the nociception and edema induced by capsaicin injection. Moreover, alpha-Spinasterol demonstrated good oral absorption and high penetration into the brain and spinal cord of mice. alpha-Spinasterol was able to displace [3H]resiniferatoxin binding and diminish calcium influx mediated by capsaicin. Oral administration of the dichloromethane fraction and alpha-Spinasterol also produced antinociceptive effect in the noxious heat-induced nociception test; however, they did not change the mechanical threshold of naive mice. The treatment with alpha-Spinasterol did not produce antinociceptive effect in mice systemically pretreated with resiniferatoxin. In addition, alpha-Spinasterol and the dichloromethane fraction reduced the edema, mechanical, and heat hyperalgesia elicited by complete Freund's adjuvant paw injection. The dichloromethane fraction and alpha-Spinasterol did not affect body temperature or locomotor activity. In conclusion, alpha-Spinasterol is a novel efficacious and safe antagonist of the TRPV1 receptor with antinociceptive effect.|
Planta Med. 2004 Aug;70(8):736-9.
|alpha-Spinasterol isolated from the root of Phytolacca americana and its pharmacological property on diabetic nephropathy.[Pubmed: 15326549 ]|
|Based on an inhibitory activity-guided fractionation for the high glucose-induced proliferation of glomerular mesangial cells (GMCs), chloroform extracts of the roots of Phytolacca americana were found to contain alpha-Spinasterol (C (29)H (48)O), a delta (7)-sterol. This phytosterol proved to be a potent inhibitor (IC (50) = 3.9 x 10 (-12) g/mL, 9.5 pmol/L) of glomerular mesangial cell proliferation caused by high-ambient glucose (5.6 mM vs. 25 mM), and its inhibitory potency was about 1,000 times higher than that of simvastatin, an HMG-CoA reductase inhibitor used as a positive control. alpha-Spinasterol also significantly reduced the increases of serum triglycerides, renal weight and urinary protein excretion in streptozotocin-induced diabetic mice, and these were comparable to the results observed in insulin-treated diabetic mice. Therefore, the results obtained in this study suggest that alpha-Spinasterol has a significant therapeutic potential to modulate the development and/or progression of diabetic nephropathy.|