|Description:||1. 4-Hydroxybenzyl alcohol possesses anti-angiogenic, anti-inflammatory and anti-
nociceptive activity possibly via its down-regulating activity on NO production, which may be partly responsible for the pharmacological efficacy of several folkloric medicines. |
2. 4-Hydroxybenzyl alcohol exhibits beneficial effects in cerebral ischemic injury, has neuroprotective effect through upregulation of Nrf2, Prdx6, and PDI expression via the PI3K/Akt pathway.
3. 4-Hydroxybenzyl alcohol inhibit the development of new blood vessels by targeting multiple mechanisms of the angiogenic process, may as a promising candidate for the establishment of anti-angiogenic treatment strategies in cancer therapy.
|Targets:||Nrf2 | PI3K | Akt | JNK | NF-kB | NO | NOS | Bcl-2/Bax | Caspase | ROS|
|Source:||The herbs of Cistanche deserticola Ma|
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||8.058 mL||40.2901 mL||80.5802 mL||161.1604 mL||201.4504 mL|
|5 mM||1.6116 mL||8.058 mL||16.116 mL||32.2321 mL||40.2901 mL|
|10 mM||0.8058 mL||4.029 mL||8.058 mL||16.116 mL||20.145 mL|
|50 mM||0.1612 mL||0.8058 mL||1.6116 mL||3.2232 mL||4.029 mL|
|100 mM||0.0806 mL||0.4029 mL||0.8058 mL||1.6116 mL||2.0145 mL|
Life Sci. 2013 Jul 19;93(1):44-50.
|4-hydroxybenzyl alcohol: a novel inhibitor of tumor angiogenesis and growth.[Pubmed: 23743167]|
|AIMS: The herbal compound 4-Hydroxybenzyl alcohol (HBA) is a pleiotropic agent, which has been shown to effectively inhibit the development of new blood vessels by targeting multiple mechanisms of the angiogenic process. Because angiogenesis is a major prerequisite for tumor growth, the aim of this study was to analyze for the first time, whether 4-Hydroxybenzyl alcohol may be used for anti-cancer therapy. MAIN METHODS: CT26.WT colon carcinoma cells were exposed to different 4-Hydroxybenzyl alcohol doses to study their viability, migration, invasiveness and protein expression compared to vehicle-treated controls. Moreover, CT26.WT cell spheroids were transplanted into the dorsal skinfold chamber of 4-Hydroxybenzyl alcohol-treated and vehicle-treated BALB/c mice for the analysis of tumor vascularization and growth by means of repetitive intravital fluorescence microscopy, histology and immunohistochemistry. KEY FINDINGS: As shown by water-soluble tetrazolium (WST)-1 and lactate dehydrogenase (LDH) assays, 4-Hydroxybenzyl alcohol treatment dose-dependently reduced the viability and integrity of the tumor cells. Moreover, phalloidin staining of 4-Hydroxybenzyl alcohol-treated cells revealed a disorganized cytoskeleton, which was associated with a decreased cellular migratory and invasive activity. Finally, 4-Hydroxybenzyl alcohol treatment inhibited the vascularization and growth of newly developing CT26.WT tumors in the mouse dorsal skinfold chamber model without affecting the normal behavior of the animals. SIGNIFICANCE: These novel findings indicate that 4-Hydroxybenzyl alcohol represents a promising candidate for the establishment of anti-angiogenic treatment strategies in cancer therapy.|
Neurochem Res. 2013 Jul;38(7):1501-16.
|4-Hydroxybenzyl alcohol confers neuroprotection through up-regulation of antioxidant protein expression.[Pubmed: 23624876]|
|An herb-derived phenolic compound, 4-Hydroxybenzyl alcohol (4-HBA), exhibits beneficial effects in cerebral ischemic injury. We demonstrated that 4-Hydroxybenzyl alcohol reduced the neuronal injury, LDH release, and up-regulation of 8-hydroxydeoxyguanosine (8-OHdG) induced by OGD/R. Furthermore, 4-Hydroxybenzyl alcohol reduced the cerebral infarct size and improved the behavioral parameters after cerebral ischemia. These neuroprotective effects may be conferred by the 4-Hydroxybenzyl alcohol mediated upregulation of the transcription factor nuclear factor E2-related factor 2 (Nrf2), peroxiredoxin 6 (Prdx6) and protein disulfide isomerase (PDI) by the use of 4-Hydroxybenzyl alcohol. Interestingly, LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor, blocked the increase in phosphorylation of Akt and abolished the neuroprotection associated with 4-Hydroxybenzyl alcohol. Our results suggested that 4-Hydroxybenzyl alcohol protects neurons against cerebral ischemic injury, and this neuroprotection may occur through upregulation of Nrf2, Prdx6, and PDI expression via the PI3K/Akt pathway.|
J Ethnopharmacol. 2012 Jan 31;139(2):549-57.
|Modulation of LPS-stimulated neuroinflammation in BV-2 microglia by Gastrodia elata: 4-hydroxybenzyl alcohol is the bioactive candidate.[Pubmed: 22155394]|
|GE extract inhibited LPS-stimulated production of inflammatory cytokines and down regulated the c-Jun NH(2)-Terminal Kinase (JNK) and nuclear factor-kappa B (NF-κB) signaling pathways, which are known to be involved in neuroinflammation. Further, inhibition of NO and iNOS by 4-Hydroxybenzyl alcohol (4-HBA), one of the active constituent of GE in LPS-stimulated BV-2 cells suggest that 4-Hydroxybenzyl alcohol might be the bioactive candidate. CONCLUSION: GE extract and its active constituent 4-Hydroxybenzyl alcohol could be further exploited to mitigate microglial activation and may be developed as a new therapeutic remedy in treating various neuroinflammatory diseases.|
Br J Pharmacol. 2011 Jun;163(4):835-44.
|In vitro and in vivo evaluation of the anti-angiogenic actions of 4-hydroxybenzyl alcohol.[Pubmed: 21366552]|
|BACKGROUND AND PURPOSE: 4-Hydroxybenzyl alcohol (HBA) is a phenolic plant compound, which has been shown to influence many cellular mechanisms. In the present study, we analysed in vitro and in vivo the anti-angiogenic actions of this pleiotropic agent. EXPERIMENTAL APPROACH: Migration and protein expression of 4-Hydroxybenzyl alcohol- and vehicle-treated endothelial-like eEND2 cells was assessed by cell migration assay and Western blot analyses. 4-Hydroxybenzyl alcohol action on vascular sprouting was analysed in an aortic ring assay. In vivo anti-angiogenic actions of 4-Hydroxybenzyl alcohol were studied in the dorsal skinfold chamber model of endometriosis in mice. KEY RESULTS: Western blot analyses demonstrated that 4-Hydroxybenzyl alcohol inhibited proliferation of eEND2 cells, as indicated by down-regulation of proliferating cell nuclear antigen expression, and reduced expression of vascular endothelial growth factor and matrix metalloproteinase 9. 4-Hydroxybenzyl alcohol suppressed the migration of eEND2 cells, accompanied by inhibition of actin filament reorganization, revealed by fluorescence staining of the cytoskeleton. In addition, 4-Hydroxybenzyl alcohol reduced vascular sprouting in the aortic ring assay. Finally, we found, in the dorsal skinfold chamber model in vivo using intravital fluorescence microscopy, that 4-Hydroxybenzyl alcohol inhibited the vascularization of developing endometriotic lesions, as indicated by a decreased functional capillary density of lesions in 4-Hydroxybenzyl alcohol-treated mice and a reduced lesion size, compared with control animals. CONCLUSIONS AND IMPLICATIONS: 4-Hydroxybenzyl alcohol targets several angiogenic mechanisms and therefore represents a promising anti-angiogenic agent for the treatment of angiogenic diseases, such as endometriosis.|
Brain Res. 2010 Jan 13;1308:167-75.
|Neuroprotective effect of 4-hydroxybenzyl alcohol against transient focal cerebral ischemia via anti-apoptosis in rats.[Pubmed: 19857470]|
|4-Hydroxybenzyl alcohol (4-HBA), one of the major active phenolic constituents of Gastrodia elata Blume, a very important traditional Chinese medicinal herb, has been shown to be an effective agent against the central and peripheral nervous disorders. In this study, we attempted to explore the possible mechanisms underlying the neuroprotection against transient focal cerebral ischemia by 4-Hydroxybenzyl alcohol.4-Hydroxybenzyl alcohol(25, 50 mg/kg) was given 30 min before focal ischemia in rats caused by middle cerebral artery occlusion (1 h of occlusion, 24 h of reperfusion). Neurological evaluation and Nissl-staining of the 4-Hydroxybenzyl alcohol group were improved significantly compared to the untreated ischemia group. TUNEL-positive cells were reduced significantly in 4-Hydroxybenzyl alcohol treated group. Results of immunofluorescence staining analysis and Western immunoblot indicated that 4-Hydroxybenzyl alcohol increased the expression of Bcl-2 and inhibited the activation of caspase-3 ultimately inhibiting apoptosis. These results suggested that 4-Hydroxybenzyl alcohol ameliorated ischemic injury induced by transient focal cerebral ischemia in rats, and this neuroprotective effect may be partly related to attenuate apoptosis pathway.|
J Pharm Pharmacol. 2007 Sep;59(9):1235-40.
|Anti-angiogenic, anti-inflammatory and anti-nociceptive activity of 4-hydroxybenzyl alcohol.[Pubmed: 17883894 ]|
|4-Hydroxybenzyl alcohol (HBA), one of the well-known phenolic compounds in diverse plants, displayed a significant inhibition in the chick chorioallantoic membrane (CAM) angiogenesis assay. HBA was shown to contain an anti-inflammatory activity in carrageenan-induced air pouch model in rats and acetic acid-induced permeability model in mice. Anti-nociceptive activity of HBA was also assessed using the acetic acid-induced writhing test in mice. HBA was able to suppress production of nitric oxide (NO) and expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-activated RAW264.7 macrophages. In the macrophages, the level of reactive oxygen species (ROS) was diminished by HBA. Taken together, HBA possesses anti-angiogenic, anti-inflammatory and anti-nociceptive activity possibly via its down-regulating activity on NO production, which may be partly responsible for the pharmacological efficacy of several folkloric medicines.|