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    Aconine
    Information
    CAS No. 509-20-6 Price $448 / 20mg
    Catalog No.CFN90248Purity>=98%
    Molecular Weight499.60Type of CompoundAlkaloids
    FormulaC25H41NO9Physical DescriptionPowder
    Download Manual    COA    MSDSSimilar structuralComparison (Web)
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    Biological Activity
    Description: Aconine can inhibit RANKL-induced osteoclast differentiation in RAW264.7 cells by suppressing NF-κB and NFATc1 activation and DC-STAMP expression. Aconine can attenuate hepatic fat degeneration of rats with fatty liver induced by high-fat diet through decreasing TG,TC deposit in liver.
    Targets: NF-kB | NOS | MMP(e.g.TIMP)
    In vitro:
    Zhongguo Zhong Yao Za Zhi. 2014 Dec;39(24):4798-803.
    [Effects of steaming and baking on content of alkaloids in Aconite Lateralis Radix (Fuzi)].[Pubmed: 25898581]
    To study the effect of steaming and baking process on contents of alkaloids in Aconite Lateralis Radix (Fuzi),
    METHODS AND RESULTS:
    13 alkaloids were analyzed by UPLC-MS/MS equipped with ESI ion source in MRM mode. In steaming process, the contents of diester-diterpenoid alkaloids decreased rapidly, the contents of monoester-diterpenoid alkaloids firstly increased, reached the peak at 40 min, and then deceased gradually. The contents of Aconine alkaloids (mesAconine, Aconine and hypAconine) increased all the time during processing, while the contents of fuziline, songorine, karacoline, salsolionl were stable or slightly decreased. In baking process, dynamic variations of alkaloids were different from that in the steaming process. Diester-diterpenoid alkaloids were degraded slightly slower than in steaming process. Monoester-diterpenoid alkaloids, Aconine alkaloids and the total alkaloids had been destroyed at different degrees, their contents were significantly lower than the ones in steaming Fuzi at the same processing time. This experiment revealed the dynamic variations of alkaloids in the course of steaming and baking.
    CONCLUSIONS:
    Two processing methods which can both effectively remove the toxic ingredients and retain the active ingredients are simple and controllable, and are valuable for popularization and application.
    Acta Pharmacol Sin. 2016 Feb; 37(2): 255–263.
    Aconine inhibits RANKL-induced osteoclast differentiation in RAW264.7 cells by suppressing NF-κB and NFATc1 activation and DC-STAMP expression.[Pubmed: 26592521]
    Aconiti Lateralis Radix Preparata is a traditional Chinese medicine used to treat chronic arthritis and is highly effective against rheumatoid arthritis. However, the effects of Aconine, a derivative of aconitum alkaloids, on osteoclasts, which can absorb bone, remain unknown. Here, we investigated the effects of Aconine on osteoclast differentiation and bone resorption in vitro.
    METHODS AND RESULTS:
    The viability of mouse leukemic monocyte/macrophage cell line RAW264.7 was measured using CCK-8 assays. Osteoclast differentiation was induced by incubation of RAW264.7 cells in the presence of RANKL, and assessed with TRAP staining assay. Bone resorption was examined with bone resorption pits assay. The expression of relevant genes and proteins was analyzed using RT-PCR and Western blots. The activation of NF-κB and nuclear factor of activated T-cells (NFAT) was examined using stable NF-κB and NFATc1 luciferase reporter gene systems, RT-PCR and Western blot analysis. Aconine (0.125, 0.25 μmol/L) did not affect the viability of RAW264.7 cells, but dose-dependently inhibited RANKL-induced osteoclast formation and bone resorptive activity. Furthermore, Aconine dose-dependently inhibited the RANKL-induced activation of NF-κB and NFATc1 in RAW264.7 cells, and subsequently reduced the expression of osteoclast-specific genes (c-Src, β3-Integrin, cathepsin K and MMP-9) and the expression of dendritic cell-specific transmembrane protein (DC-STAMP), which played an important role in cell-cell fusion.
    CONCLUSIONS:
    These findings suggest that Aconine inhibits RANKL-induced osteoclast differentiation in RAW264.7 cells by suppressing the activation of NF-κB and NFATc1 and the expression of the cell-cell fusion molecule DC-STAMP.
    Aconine Description
    Source: The roots of Aconitum kusnezoffii Reichb.
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 2.0016 mL 10.008 mL 20.016 mL 40.032 mL 50.04 mL
    5 mM 0.4003 mL 2.0016 mL 4.0032 mL 8.0064 mL 10.008 mL
    10 mM 0.2002 mL 1.0008 mL 2.0016 mL 4.0032 mL 5.004 mL
    50 mM 0.04 mL 0.2002 mL 0.4003 mL 0.8006 mL 1.0008 mL
    100 mM 0.02 mL 0.1001 mL 0.2002 mL 0.4003 mL 0.5004 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Structure Identification:
    Nat Prod Commun. 2014 Jun;9(6):785-6.
    Ring A conformation of aconine and pseudaconine in CDCl3.[Pubmed: 25115078]

    METHODS AND RESULTS:
    On the basis of intensive interpretation of the 1H NMR spectroscopic data, the ring A conformation of Aconine (1) was speculated as twist boat in CDCl3, and as chair or twist boat in acetone-d6 and pyridine-d5. The ring A of pseudAconine (2) adopts the chair conformation in CDCl3, acetone-d6, and pyridine-ds. Accordingly, the boat conformation of ring A in these two diterpenoid alkaloids in CDCl3 reported in the literature [1] should be revised.
    CONCLUSIONS:
    The difference in 13C NMR data for the same compound (1 or 2) in two different solvents (CDCl3, pyridine-d5) can be attributed to solvent effects.