• ChemFaces is a professional high-purity natural products manufacturer.
  • Product Intended Use
  • 1. Reference standards
  • 2. Pharmacological research
  • 3. Inhibitors
  • Home
  • Natural Products
  • Bioactive
  • Screening Libraries
  • Hot Products
  • Plant Catalog
  • Customer Support
  • Product Use Citation
  • About Us
  • Contact Us
  • Natural Products
    Anemarsaponin B
    Information
    CAS No. 139051-27-7 Price $248 / 20mg
    Catalog No.CFN99532Purity>=98%
    Molecular Weight903.04Type of CompoundSteroids
    FormulaC45H74O18Physical DescriptionPowder
    Download Manual    COA    MSDSSimilar structuralComparison (Web)
    How to Order
    Orders via your E-mail:

    1. Product number / Name / CAS No.
    2. Delivery address
    3. Ordering/billing address
    4. Contact information
    Sent to Email: info@chemfaces.com
    Contact Us
    Order & Inquiry & Tech Support

    Tel: (0086)-27-84237683
    Fax: (0086)-27-84254680
    E-mail: manager@chemfaces.com
    Address: No. 83, CheCheng Rd., WETDZ, Wuhan, Hubei 430056, PRC
    Delivery time
    Delivery & Payment method

    1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

    2. We accept: Wire transfer & Credit card & Paypal & Western Union
    * Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
    Our products had been exported to the following research institutions and universities, And still growing.
  • Chulalongkorn University (Thailand)
  • University of Mysore (India)
  • University of Wollongong (Australia)
  • Universidade da Beira Interior (Germany)
  • University of Toronto (Canada)
  • Florida A&M University (USA)
  • Complutense University of Madrid (Spain)
  • University of Limpopo (South Africa)
  • National Cancer Center Research ... (Japan)
  • Julius Kühn-Institut (Germany)
  • University of Dicle (Turkey)
  • More...
  • Package
    Featured Products
    Kaempferol-3-O-galactoside

    Catalog No: CFN92079
    CAS No: 23627-87-4
    Price: $398/5mg
    Gnetol

    Catalog No: CFN92425
    CAS No: 86361-55-9
    Price: $388/5mg
    Polygalaxanthone III

    Catalog No: CFN90208
    CAS No: 162857-78-5
    Price: $138/20mg
    Pd-C-II

    Catalog No: CFN95003
    CAS No: N/A
    Price: $358/5mg
    Kumatakenin

    Catalog No: CFN98423
    CAS No: 3301-49-3
    Price: $238/10mg
    Biological Activity
    Description: Anemarsaponin B can inhibit PAF-induced rabbit platelet aggregation in vitro. Anemarsaponin B has anti-inflammatory effect in LPS-treated RAW 264.7macrophages, the effect is associated with the inhibition of NF-κB transcriptional activity, possibly via the p38 MAP kinase pathway.
    Targets: NOS | COX | p38MAPK | IL Receptor | TNF-α | NF-kB
    In vitro:
    Yao Xue Xue Bao. 1992;27(1):26-32.
    [Studies on the active constituents of Anemarrhena asphodeloides bunge].[Pubmed: 1529709]

    METHODS AND RESULTS:
    From the ethanol extract of the Anemarrhena asphodeloides Buge (Liliaceae), a new steroidal suponin Anemarsaponin B and two known saponins anemarsaponin A1 and A2 were isolated. On the basis of chemical evidences and spectral analysis (UV, IR, EI-MS, FAB-MS, 1H-NMR and 13C-NMR), the structure of Anemarsaponin B was elucidated as 26-O-beta-D-glucopyranosylfurost-20(22)-ene-3 beta,26-diol-3-O-beta-D- glucopyranosyl(1----2)-beta-D-galactopyranoside.
    CONCLUSIONS:
    Preliminary pharmacological tests showed that Anemarsaponin B could inhibit PAF-induced rabbit platelet aggregation in vitro.
    Planta Med. 1991 Oct;57(5):460-2.
    A new active steroidal saponin from Anemarrhena asphodeloides.[Pubmed: 1798801]

    METHODS AND RESULTS:
    A new active steroidal saponin, Anemarsaponin B, was isolated from the rhizomes of Anemarrhina asphodeloides. The structure of Anemarsaponin B was elucidated as 26-O-beta-D-glucopyranosylfurost-20(22)-ene-3 beta, 26-diol-3-O-beta-D-glucopyranosyl-(1----2)-beta-D-galactopyranoside by chemical and spectral studies.
    CONCLUSIONS:
    Preliminary pharmacological tests showed that Anemarsaponin B could inhibit PAF-induced rabbit platelet aggregation in vitro.
    Anemarsaponin B Description
    Source: The rhizomes of Anemarrhena asphodeloides Bunge.
    Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
    Recent ChemFaces New Products and Compounds
    Buddlenoid A

    Catalog No: CFN95025
    CAS No: 142750-32-1
    Price: $368/5mg
    Carasiphenol C

    Catalog No: CFN95045
    CAS No: 868168-04-1
    Price: $368/5mg
    Glycitein

    Catalog No: CFN99106
    CAS No: 40957-83-3
    Price: $128/20mg
    Lucidumol A

    Catalog No: CFN95059
    CAS No: 217476-73-8
    Price: $413/5mg
    2-Cinnamoyl-1-galloylglucose

    Catalog No: CFN95098
    CAS No: 56994-83-3
    Price: $338/5mg
    Pyrocurzerenone

    Catalog No: CFN96169
    CAS No: 20013-75-6
    Price: $388/20mg
    Columbianetin

    Catalog No: CFN90185
    CAS No: 3804-70-4
    Price: $268/20mg
    Arnicolide C

    Catalog No: CFN91996
    CAS No: 34532-67-7
    Price: $398/5 mg
    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.

    PMID: 29328914

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.

    PMID: 29149595

    Scientific Reports 2017 Dec 11;7(1):17332.
    doi: 10.1038/s41598-017-17427-6.

    PMID: 29230013

    Molecules. 2017 Oct 27;22(11). pii: E1829.
    doi: 10.3390/molecules22111829.

    PMID: 29077044

    J Cell Biochem. 2018 Feb;119(2):2231-2239.
    doi: 10.1002/jcb.26385.

    PMID: 28857247

    Phytomedicine. 2018 Feb 1;40:37-47.
    doi:10.1016/j.phymed.2017.12.030

    PMID: 29496173
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.1074 mL 5.5369 mL 11.0737 mL 22.1474 mL 27.6843 mL
    5 mM 0.2215 mL 1.1074 mL 2.2147 mL 4.4295 mL 5.5369 mL
    10 mM 0.1107 mL 0.5537 mL 1.1074 mL 2.2147 mL 2.7684 mL
    50 mM 0.0221 mL 0.1107 mL 0.2215 mL 0.4429 mL 0.5537 mL
    100 mM 0.0111 mL 0.0554 mL 0.1107 mL 0.2215 mL 0.2768 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Kinase Assay:
    Food Chem Toxicol. 2009 Jul;47(7):1610-7.
    Anti-inflammatory effect of anemarsaponin B isolated from the rhizomes of Anemarrhena asphodeloides in LPS-induced RAW 264.7 macrophages is mediated by negative regulation of the nuclear factor-kappaB and p38 pathways.[Pubmed: 19375480]
    Anemarrhena asphodeloides is widely used in traditional Chinese medicine, and is known to have anti-diabetic and diuretic effects.
    METHODS AND RESULTS:
    In this study, we evaluated the anti-inflammatory effects of Anemarsaponin B (ASB), a steroidal saponin isolated from the rhizomes of A. asphodeloides (Liliaceae), in LPS-stimulated RAW 264.7 macrophage cell line. ASB significantly and dose-dependently decreased the protein and mRNA levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). ASB also reduced the expressions and productions of pro-inflammatory cytokines, including those of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6). Electrophoretic mobility shift assay (EMSA) and reporter gene assays revealed that ASB attenuated the LPS-induced DNA binding and transcriptional activity of nuclear factor-kappa B (NF-kappaB). In addition, it was found that pretreatment with ASB significantly inhibited the nuclear translocation of the p65 subunit of NF-kappaB by blocking the phosphorylation of inhibitory kappa B-alpha (IkappaB-alpha). On the other hand, ASB inhibited the phosphorylation of MAP kinase kinases 3/6 (MKK3/6) and mixed lineage kinase 3 (MLK3), which are both involved in the p38 pathway.
    CONCLUSIONS:
    Taken together, these results suggest that anti-inflammatory effect of ASB in LPS-treated RAW 264.7 macrophages is associated with the inhibition of NF-kappaB transcriptional activity, possibly via the p38 MAP kinase pathway.