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Anemarsaponin B
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Product Name Anemarsaponin B
Price: $318 / 20mg
CAS No.: 139051-27-7
Catalog No.: CFN99532
Molecular Formula: C45H74O18
Molecular Weight: 903.04 g/mol
Purity: >=98%
Type of Compound: Steroids
Physical Desc.: Powder
Source: The rhizomes of Anemarrhena asphodeloides Bunge.
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Download: COA    MSDS    SDF    Manual
Similar structural: Comparison (Web)  (SDF)
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According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $206.7 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
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Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Anemarsaponin B can inhibit PAF-induced rabbit platelet aggregation in vitro. Anemarsaponin B has anti-inflammatory effect in LPS-treated RAW 264.7macrophages, the effect is associated with the inhibition of NF-κB transcriptional activity, possibly via the p38 MAP kinase pathway.
Targets: NOS | COX | p38MAPK | IL Receptor | TNF-α | NF-kB
In vitro:
Yao Xue Xue Bao. 1992;27(1):26-32.
[Studies on the active constituents of Anemarrhena asphodeloides bunge].[Pubmed: 1529709]

METHODS AND RESULTS:
From the ethanol extract of the Anemarrhena asphodeloides Buge (Liliaceae), a new steroidal suponin Anemarsaponin B and two known saponins anemarsaponin A1 and A2 were isolated. On the basis of chemical evidences and spectral analysis (UV, IR, EI-MS, FAB-MS, 1H-NMR and 13C-NMR), the structure of Anemarsaponin B was elucidated as 26-O-beta-D-glucopyranosylfurost-20(22)-ene-3 beta,26-diol-3-O-beta-D- glucopyranosyl(1----2)-beta-D-galactopyranoside.
CONCLUSIONS:
Preliminary pharmacological tests showed that Anemarsaponin B could inhibit PAF-induced rabbit platelet aggregation in vitro.
Planta Med. 1991 Oct;57(5):460-2.
A new active steroidal saponin from Anemarrhena asphodeloides.[Pubmed: 1798801]

METHODS AND RESULTS:
A new active steroidal saponin, Anemarsaponin B, was isolated from the rhizomes of Anemarrhina asphodeloides. The structure of Anemarsaponin B was elucidated as 26-O-beta-D-glucopyranosylfurost-20(22)-ene-3 beta, 26-diol-3-O-beta-D-glucopyranosyl-(1----2)-beta-D-galactopyranoside by chemical and spectral studies.
CONCLUSIONS:
Preliminary pharmacological tests showed that Anemarsaponin B could inhibit PAF-induced rabbit platelet aggregation in vitro.
Food Chem Toxicol . 2009 Jul;47(7):1610-7.
Anti-inflammatory effect of anemarsaponin B isolated from the rhizomes of Anemarrhena asphodeloides in LPS-induced RAW 264.7 macrophages is mediated by negative regulation of the nuclear factor-kappaB and p38 pathways[Pubmed: 19375480]
Abstract Anemarrhena asphodeloides is widely used in traditional Chinese medicine, and is known to have anti-diabetic and diuretic effects. In this study, we evaluated the anti-inflammatory effects of Anemarsaponin B (ASB), a steroidal saponin isolated from the rhizomes of A. asphodeloides (Liliaceae), in LPS-stimulated RAW 264.7 macrophage cell line. ASB significantly and dose-dependently decreased the protein and mRNA levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). ASB also reduced the expressions and productions of pro-inflammatory cytokines, including those of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6). Electrophoretic mobility shift assay (EMSA) and reporter gene assays revealed that ASB attenuated the LPS-induced DNA binding and transcriptional activity of nuclear factor-kappa B (NF-kappaB). In addition, it was found that pretreatment with ASB significantly inhibited the nuclear translocation of the p65 subunit of NF-kappaB by blocking the phosphorylation of inhibitory kappa B-alpha (IkappaB-alpha). On the other hand, ASB inhibited the phosphorylation of MAP kinase kinases 3/6 (MKK3/6) and mixed lineage kinase 3 (MLK3), which are both involved in the p38 pathway. Taken together, these results suggest that anti-inflammatory effect of ASB in LPS-treated RAW 264.7 macrophages is associated with the inhibition of NF-kappaB transcriptional activity, possibly via the p38 MAP kinase pathway.
Anemarsaponin B Description
Source: The rhizomes of Anemarrhena asphodeloides Bunge.
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.1074 mL 5.5369 mL 11.0737 mL 22.1474 mL 27.6843 mL
5 mM 0.2215 mL 1.1074 mL 2.2147 mL 4.4295 mL 5.5369 mL
10 mM 0.1107 mL 0.5537 mL 1.1074 mL 2.2147 mL 2.7684 mL
50 mM 0.0221 mL 0.1107 mL 0.2215 mL 0.4429 mL 0.5537 mL
100 mM 0.0111 mL 0.0554 mL 0.1107 mL 0.2215 mL 0.2768 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Kinase Assay:
Food Chem Toxicol. 2009 Jul;47(7):1610-7.
Anti-inflammatory effect of anemarsaponin B isolated from the rhizomes of Anemarrhena asphodeloides in LPS-induced RAW 264.7 macrophages is mediated by negative regulation of the nuclear factor-kappaB and p38 pathways.[Pubmed: 19375480]
Anemarrhena asphodeloides is widely used in traditional Chinese medicine, and is known to have anti-diabetic and diuretic effects.
METHODS AND RESULTS:
In this study, we evaluated the anti-inflammatory effects of Anemarsaponin B (ASB), a steroidal saponin isolated from the rhizomes of A. asphodeloides (Liliaceae), in LPS-stimulated RAW 264.7 macrophage cell line. ASB significantly and dose-dependently decreased the protein and mRNA levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). ASB also reduced the expressions and productions of pro-inflammatory cytokines, including those of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6). Electrophoretic mobility shift assay (EMSA) and reporter gene assays revealed that ASB attenuated the LPS-induced DNA binding and transcriptional activity of nuclear factor-kappa B (NF-kappaB). In addition, it was found that pretreatment with ASB significantly inhibited the nuclear translocation of the p65 subunit of NF-kappaB by blocking the phosphorylation of inhibitory kappa B-alpha (IkappaB-alpha). On the other hand, ASB inhibited the phosphorylation of MAP kinase kinases 3/6 (MKK3/6) and mixed lineage kinase 3 (MLK3), which are both involved in the p38 pathway.
CONCLUSIONS:
Taken together, these results suggest that anti-inflammatory effect of ASB in LPS-treated RAW 264.7 macrophages is associated with the inhibition of NF-kappaB transcriptional activity, possibly via the p38 MAP kinase pathway.
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