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    Epibrassinolide
    Epibrassinolide
    Information
    CAS No. 72962-43-7 Price
    Catalog No.CFN80268Purity>=98%
    Molecular Weight480.34Type of CompoundSteroids
    FormulaC28H48O6Physical DescriptionPowder
    Download     COA    MSDSSimilar structuralComparison (Web)
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    Epibrassinolide Description
    Source: The herbs of Brassica napus
    Biological Activity or Inhibitors: 1. Epibrassinolide restores the auxin responsiveness ofdiageotropica hypocotyls with respect to elongation.
    2. Epibrassinolide is active against herpes and arena viruses.
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.

    PMID: 29328914

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.

    PMID: 29149595

    Scientific Reports 2017 Dec 11;7(1):17332.
    doi: 10.1038/s41598-017-17427-6.

    PMID: 29230013

    Molecules. 2017 Oct 27;22(11). pii: E1829.
    doi: 10.3390/molecules22111829.

    PMID: 29077044

    J Cell Biochem. 2018 Feb;119(2):2231-2239.
    doi: 10.1002/jcb.26385.

    PMID: 28857247

    Phytomedicine. 2018 Feb 1;40:37-47.
    doi:10.1016/j.phymed.2017.12.030

    PMID: 29496173
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 2.0819 mL 10.4093 mL 20.8186 mL 41.6372 mL 52.0465 mL
    5 mM 0.4164 mL 2.0819 mL 4.1637 mL 8.3274 mL 10.4093 mL
    10 mM 0.2082 mL 1.0409 mL 2.0819 mL 4.1637 mL 5.2046 mL
    50 mM 0.0416 mL 0.2082 mL 0.4164 mL 0.8327 mL 1.0409 mL
    100 mM 0.0208 mL 0.1041 mL 0.2082 mL 0.4164 mL 0.5205 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Epibrassinolide References Information
    Citation [1]

    Russian Journal of Plant Physiology, 2008, 55(2):193-200.

    Effect of epibrassinolide on tyrosine phosphorylation of the calvin cycle enzymes.[Reference: WebLink]
    The plants of Brassica juncea (L) were grown in the presence of NaCl and/or NiCl2 and were sprayed with 1muM of 24-Epibrassinolide (EBL) at 15 days after sowing (DAS) and were sampled at 30 DAS. The plants exposed to NaCl and/or NiCl2 exhibited a significant decline in growth, the level of pigments and photosynthetic parameters. However, the follow up treatment with EBL detoxified the stress generated by NaCl and/or NiCl2 and significantly improved the above parameters. The NaCl and/or NiCl2 increased electrolyte leakage and lipid peroxidation, and decreased the membrane stability index (MSI) and relative water content. However, the EBL treatment in absence of the stress improved the MSI and relative water content but could not influence electrolyte leakage and lipid peroxidation. The antioxidative enzymes and the level of proline exhibited a significant increase in response to EBL as well as to NaCl and/or NiCl2 stress.
    Citation [2]

    Planta. 1998 Dec;207(1):120-4.

    Effect of epibrassinolide on hypocotyl growth of the tomato mutant diageotropica.[Pubmed: 11536896 ]
    The effect of Epibrassinolide (epiBR) on the growth and gravitropism of hypocotyls was investigated in diageotropica (dgt), a mutant of tomato (Lycopersicon esculentum Mill.). The elongation of (dgt) hypocotyls, which had been reported to be auxin-insensitive, was promoted by increasing concentrations of indole-3-acetic acid (IAA) in the presence of epiBR. alpha-(p-Chlorophenoxy)isobutyric acid, an inhibitor of auxin action, blocked the enhancement of growth by IAA and epiBR. Time course analysis of IAA-induced dgt hypocotyl elongation in the presence of epiBR revealed typical auxin response kinetics. These results suggest that epiBR restores the auxin responsiveness of dgt hypocotyls with respect to elongation. However, epiBR did not rescue the dgt phenotype with respect to shoot gravitropism. It was therefore concluded that brassino-steroid insensitivity or deficiency is not the primary defect of the dgt mutation.