|Source:||The roots of Rubia cordifolia|
|Biological Activity or Inhibitors:|| 1. Lucidin and its derivatives are genotoxic, it is mutagenic at the hypoxanthine-guanine phosphoribosyl transferase gene locus.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||3.703 mL||18.5151 mL||37.0302 mL||74.0604 mL||92.5754 mL|
|5 mM||0.7406 mL||3.703 mL||7.406 mL||14.8121 mL||18.5151 mL|
|10 mM||0.3703 mL||1.8515 mL||3.703 mL||7.406 mL||9.2575 mL|
|50 mM||0.0741 mL||0.3703 mL||0.7406 mL||1.4812 mL||1.8515 mL|
|100 mM||0.037 mL||0.1852 mL||0.3703 mL||0.7406 mL||0.9258 mL|
Cell Biol Toxicol. 1988 Jun;4(2):225-39.
|The genotoxicity of lucidin, a natural component of Rubia tinctorum L., and lucidinethylether, a component of ethanolic Rubia extracts.[Pubmed: 3069188]|
|The genotoxic activity of Lucidin (1,3-dihydroxy-2-hydroxymethyl-9,10-anthraquinone), a natural component of Rubia tinctorum L., was tested in a battery of short-term tests. The compound was mutagenic in five Salmonella typhimurium strains without metabolic activation, but the mutagenicity was increased after addition of rat liver S9 mix. In V79 cells, Lucidin was mutagenic at the hypoxanthine-guanine phosphoribosyl transferase gene locus and active at inducing DNA single-strand breaks and DNA-protein cross-links as assayed by the alkaline elution method. Lucidin also induced DNA repair synthesis in primary rat hepatocytes and transformed C3H/M2-mouse fibroblasts in culture. We also investigated Lucidinethylether, which is formed from Lucidin by extraction of madder roots with boiling ethanol. This compound was also mutagenic in Salmonella, but only after addition of rat liver S9 mix. Lucidinethylether was weakly mutagenic to V79 cells which were cocultivated with rat hepatocytes. The compound did not induce DNA repair synthesis in hepatocytes from untreated rats, but positive results were obtained when hepatocytes from rats pretreated with phenobarbital were used. We conclude that Lucidin and its derivatives are genotoxic.|