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    Phyllostine
    Phyllostine
    Information
    CAS No. 27270-89-9 Price
    Catalog No.CFN97901Purity>=98%
    Molecular Weight154.1Type of CompoundQuinones
    FormulaC7H6O4Physical DescriptionYellow powder
    Download Manual    COA    MSDS    SDFSimilar structuralComparison (Web)
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  • Package
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    Phyllostine Description
    Source: From Pencillium urticae
    Biological Activity or Inhibitors: 1. Phyllostine appears to be their more immediate precursor, since PCMB-treated extracts of J2 converted Phyllostine but not isoepoxydon to these new metabolites.
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.

    PMID: 29328914

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.

    PMID: 29149595

    Scientific Reports 2017 Dec 11;7(1):17332.
    doi: 10.1038/s41598-017-17427-6.

    PMID: 29230013

    Molecules. 2017 Oct 27;22(11). pii: E1829.
    doi: 10.3390/molecules22111829.

    PMID: 29077044

    J Cell Biochem. 2018 Feb;119(2):2231-2239.
    doi: 10.1002/jcb.26385.

    PMID: 28857247

    Phytomedicine. 2018 Feb 1;40:37-47.
    doi:10.1016/j.phymed.2017.12.030

    PMID: 29496173
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 6.4893 mL 32.4465 mL 64.8929 mL 129.7859 mL 162.2323 mL
    5 mM 1.2979 mL 6.4893 mL 12.9786 mL 25.9572 mL 32.4465 mL
    10 mM 0.6489 mL 3.2446 mL 6.4893 mL 12.9786 mL 16.2232 mL
    50 mM 0.1298 mL 0.6489 mL 1.2979 mL 2.5957 mL 3.2446 mL
    100 mM 0.0649 mL 0.3245 mL 0.6489 mL 1.2979 mL 1.6223 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Phyllostine References Information
    Citation [1]

    Can J Microbiol. 1979 Aug;25(8):881-7.

    Patulin biosynthesis: the metabolism of phyllostine and isoepoxydon by cell-free preparations from Pencillium urticae.[Pubmed: 43192]
    Cell-free extracts of Penicillium urticae (NRRL 2159A), and its Pat- mutants, J2, J1, and S11, were found to contain significant NADP-dependent isoepoxydon dehydrogenase activity. This reversible interconversion of the epoxides (-)-Phyllostine and (+)-isoepoxydon occurred optimally at pH 5.8 and was completely inhibited by 1 mM p-chloromercuribenzoate (PCMB). The cytosol enzyme possessed specificity for both substrate and cofactor since neither (+)-epoxydon, an epimer of (+)-isoepoxydon, nor NADH was utilized. Cell extracts of the parent and of mutant J2, which is blocked before the epoxides in the patulin pathway, were found to convert Phyllostine and isoepoxydon to a number of unknown metabolites which appeared as yellow spots on thin-layer chromatograms after spraying with a chromogenic reagent. Extracts of mutant J1 were unable to carry out this conversion, while whole cells of mutant S11 accumulated what appeared to be these same 'yellow' compounds. Since PCMB-treated extracts of J2 converted Phyllostine but not isoepoxydon to these new metabolites, Phyllostine appeared to be their more immediate precursor. The relative positions of isoepoxydon and Phyllostine in the patulin pathway are discussed.