|Source:||The leaves and twigs of Securinega suffruticosa.|
|Biological Activity or Inhibitors:||1. Securinine induces apoptosis in human leukemia HL-60 cells.
2. Securinine is a promising new monocytic differentiation inducing agent for Acute Myeloid Leukemia (AML) .
3. Securinine, a GABAA receptor antagonist, enhances macrophage clearance of phase II C. burnetii: comparison with TLR agonists.
4. Securinine shows antifungal activity against some plant pathogenic fungi.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||4.6028 mL||23.0139 mL||46.0278 mL||92.0556 mL||115.0695 mL|
|5 mM||0.9206 mL||4.6028 mL||9.2056 mL||18.4111 mL||23.0139 mL|
|10 mM||0.4603 mL||2.3014 mL||4.6028 mL||9.2056 mL||11.507 mL|
|50 mM||0.0921 mL||0.4603 mL||0.9206 mL||1.8411 mL||2.3014 mL|
|100 mM||0.046 mL||0.2301 mL||0.4603 mL||0.9206 mL||1.1507 mL|
Zhongguo Yao Li Xue Bao. 1999 Mar;20(3):267-70.
|Securinine induced apoptosis in human leukemia HL-60 cells.[Pubmed: 10452105]|
|To study whether Securinine might induce apoptosis in human leukemia HL-60 cells. METHODS: Inhibition of proliferation was measured using MTT assay. The amount of apoptotic cells was measured by flow cytometry. DNA fragmentation was visualized by DNA agarose gel electrophoresis and the cellular changes were observed by electron microscope. RESULTS: Securinine 5-80 mg.L-1 elicited typical apoptosis morphological changes and DNA fragmentation in a concentration-dependent manner in HL-60 cells. Securinine inhibited HL-60 cell proliferation in a time- and concentration-dependent manner. The IC50 and 95% confidence limits were 27 (15-47) mg.L-1 after 12-h treatment with Securinine. CONCLUSION: Securinine induced apoptosis in HL-60 cells.|
PLoS One. 2011;6(6):e21203.
|Securinine, a myeloid differentiation agent with therapeutic potential for AML.[Pubmed: 21731671]|
|As the defining feature of Acute Myeloid Leukemia (AML) is a maturation arrest, a highly desirable therapeutic strategy is to induce leukemic cell maturation. This therapeutic strategy has the potential of avoiding the significant side effects that occur with the traditional AML therapeutics. We identified a natural compound Securinine, as a leukemia differentiation-inducing agent. Securinine is a plant-derived alkaloid that has previously been used clinically as a therapeutic for primarily neurological related diseases. Securinine induces monocytic differentiation of a wide range of myeloid leukemia cell lines as well as primary leukemic patient samples. Securinine's clinical potential for AML can be seen from its ability to induce significant growth arrest in cell lines and patient samples as well as its activity in significantly impairing the growth of AML tumors in nude mice. In addition, Securinine can synergize with currently employed agents such as ATRA and decitabine to induce differentiation. This study has revealed Securinine induces differentiation through the activation of DNA damage signaling. Securinine is a promising new monocytic differentiation inducing agent for AML that has seen previous clinical use for non-related disorders.|
J. Leukoc. Biol.,2007, 82(5):1062-1069.
|Securinine, a GABAA receptor antagonist, enhances macrophage clearance of phase II C. burnetii: comparison with TLR agonists.[Reference: WebLink]|
|Innate immune cell stimulation represents a complementary approach to vaccines and antimicrobial drugs to counter infectious disease. We have used assays of macrophage activation and in vitro and in vivo phase II Coxiella burnetii infection models to compare and contrast the activity of a novel innate immune cell agonist, Securinine, with known TLR agonists. As expected, TLR agonists, such as LPS (TLR4) and fibroblast-stimulating lipopeptide-1 (FSL-1; TLR2), induced macrophage activation and increased macrophage killing of phase II C. burnetii in vitro. FSL-1 also induced accelerated killing of C. burnetii in vivo. Securinine, a γ-aminobutyric acid type A receptor antagonist, was found to induce TLR-independent macrophage activation in vitro, leading to IL-8 secretion, L-selectin down-regulation, and CD11b and MHC Class II antigen up-regulation. As seen with the TLR agonists, Securinine also induced accelerated macrophage killing of C. burnetii in vitro and in vivo. In summary, as predicted by the literature, TLR agonists enhance macrophage killing of phase II C. burnetii in vitro, and at least for TLR2 agonists, this activity occurs in vivo as well. Securinine represents a novel macrophage agonist, which has similar effects as TLR agonists in this model yet apparently, does not act through known TLRs. Securinine has minimal toxicity in vivo, suggesting it or structurally similar compounds may represent novel, therapeutic adjuvants, which increase resistance to intracellular pathogens.|
Mycobiology. 2008 Jun;36(2):99-101.
|Antifungal Activity of Securinine against Some Plant Pathogenic Fungi.[Pubmed: 23990741 ]|
|The alkaloid Securinine was assessed against spore germination of some plant pathogenic and saprophytic fungi (Alternaria alternata, Alternaria brassicae, Alternaria brassicicola, Curvularia lunata, Curvularia maculans, Curvularia pallenscens, Colletotrichum musae, Colletotrichum sp., Erysiphe pisi, Helminthosporium echinoclova, Helminthosporium spiciferum, Heterosporium sp.). Spore germinations of all the tested fungi were inhibited. Alternaria brassicicola, C. lunata, C. pallenscens and H. spiciferum were highly sensitive as complete inhibition of spore germination was observed at very low concentrations (200 ppm).|
Chinese Journal of Natural Medicines, 2006, 4(4):260-263.
|Chemical constituents of Securinega suffruticosa[Reference: WebLink]|
|To investigate the chemical constituents of the leaves and twigs of Securinega suffruticosa (Pall.) Rehd. Method: Column chromatographic methods were used to separate the chemical constituents whose structures were elucidated by means of physicochemical properties and spectral analysis. Result: Eleven compounds were isolated and identified as (-)-15β-ethoxy-14,15-dihydroviroalloSecurinine (1), Securinine (2), 14,15-dihydroSecurinine (3), 4-epiphyllanthine (4), Securitinine (5), viroalloSecurinine (6), Securinol A (7), secu' amamine A (8), ent-phyllanthidine (9), (+)-aquilegiolide (10) and (+)-menisdaurilide (11), respectively. Conclusion: Compound 1 is a novel compound. Compounds 4, 8, 10 and 11 were isolated from Securinega suffruticosa for the first time.|