|Source:||The roots of Polygonum multiflorum|
|Biological Activity or Inhibitors:||1. 2,3,5,4'-Tetrahydroxyldiphenylethylene-2-O-glucoside(TSG) blocks the proliferation of PASMCs induced by PDGF-BB through arresting the cell cycle in G0 / G1 phase, indicates that TSG suppress PDGF-BB-induced PASMCs proliferation is associated with an inhibition of AKT / GSK3β signaling pathways.
2. TSG not only prevents over-expression of α-synuclein at an early stage, but also reverses the increased expression of α-synuclein and inhibits the aggregation at the late stage of Tg mice, it may have potential to the prevention and treatment of Alzheimer's diseases.
3. TGS, emodin and physcion have the lipid regulation activity to an overall synergy.
|Solvent:||DMSO, Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.4607 mL||12.3035 mL||24.6069 mL||49.2138 mL||61.5173 mL|
|5 mM||0.4921 mL||2.4607 mL||4.9214 mL||9.8428 mL||12.3035 mL|
|10 mM||0.2461 mL||1.2303 mL||2.4607 mL||4.9214 mL||6.1517 mL|
|50 mM||0.0492 mL||0.2461 mL||0.4921 mL||0.9843 mL||1.2303 mL|
|100 mM||0.0246 mL||0.123 mL||0.2461 mL||0.4921 mL||0.6152 mL|
Restor Neurol Neurosci. 2013;31(1):41-52.
|Tetrahydroxystilbene glucoside antagonizes age-related α-synuclein overexpression in the hippocampus of APP transgenic mouse model of Alzheimer's disease.[Pubmed: 23160059]|
|The expression and aggregation of α-synuclein was age-dependently increased in Tg mice. 2,3,5,4'-Tetrahydroxyl diphenylethylene-2-O-glucoside not only prevents over-expression of α-synuclein at an early stage, but also reverses the increased expression of α-synuclein and inhibits the aggregation at the late stage of Tg mice. TSG may have potential to the prevention and treatment of Alzheimer's diseases.|
J Ethnopharmacol. 2011 Oct 11;137(3):1291-9.
|Hepatoxicity of major constituents and extractions of Radix Polygoni Multiflori and Radix Polygoni Multiflori Praeparata.[Pubmed: 21840387]|
|In conclusion, Radix Polygonum multiflorum and Radix Polygonum multiflorum Praeparata were not liver injure inducing in our in vitro assays. However, the processing produce of RPM could reduce its effect on both cell proliferation and enzyme secretion of liver cell. Judging from cell proliferation, integrity of cell membrane and enzyme secretion, three major chemical constituents of RPM: 2,3,5,4'-Tetrahydroxyl diphenylethylene-2-O-glucoside, physcion and emodin showed no, moderate and severe cytotoxicity against human liver cell line L-02 respectively. Chemical constituents-cytotoxicity relationship investigation revealed that TSG and physcion probably had attenuating effect to emodin. The attenuating mechanisms were still under investigation.|
J Ethnopharmacol. 2014 May 14;153(3):763-70.
|In vitro effects of active components of Polygonum Multiflorum Radix on enzymes involved in the lipid metabolism.[Pubmed: 24680992]|
|2,3,5,4'-Tetrahydroxyl diphenylethylene-2-O-glucoside could raise the content of CYP7A and then promote the lipolysis of cholesterol. Moreover, 2,3,5,4'-Tetrahydroxyl diphenylethylene-2-O-glucoside also showed the best LDL-reducing effect. Emodin could inhibit HMG-CoA reductase and DGAT1, which were key enzymes in the synthesis of TC and TG. Physcion increased the content of HTGL, and then could boost the lipolysis of triglyceride. At the same time, physcion showed the best VLDL-reducing effect. In view of the above conclusions, we contributed the lipid regulation activity to an overall synergy of 2,3,5,4'-Tetrahydroxyl diphenylethylene-2-O-glucoside, emodin and physcion.|
Gene. 2014 Aug 15;547(1):126-35.
|Transcriptome profiling and digital gene expression analysis of Fallopia multiflora to discover putative genes involved in the biosynthesis of 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside.[Pubmed: 24967942]|
|The compound 2,3,5,4'-Tetrahydroxyl diphenylethylene-2-O-glucoside (THSG) synthesized by Fallopia multiflora (F. multiflora) exhibits pharmacological potency. However, the mechanistic details of its biosynthesis pathway are still vague. To clear this ambiguity, we performed de novo transcriptome assembly and digital gene expression (DGE) profiling analyses of F. multiflora using the Illumina RNA-seq system. RNA-seq generated approximately 70 million high-quality reads that were assembled into 65,653 unigenes (mean length=750 bp), including 26,670 clusters and 38,983 singletons. A total of 48,173 (73.4%) unigenes were annotated using public protein databases with a cut-off e-value above 10(-5). Furthermore, we investigated the transcriptome difference of four different F. multiflora tissues using DGE profiling. Variations in gene expression were identified based on comparisons of transcriptomes from various parts of a high-level THSG- and a low-level THSG-producing F. multiflora plant. Clusters with similar differential expression patterns and enriched metabolic pathways with regard to the differentially expressed genes putatively involved in THSG biosynthesis were revealed for the first time. Our data provides the most comprehensive sequence resource regarding F. multiflora so far. Taken together, the results of this study considerably extend the knowledge on THSG production.|