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    20-Hydroxyecdysone
    Information
    CAS No. 5289-74-7 Price $50 / 20mg
    Catalog No.CFN98873Purity>=98%
    Molecular Weight480.6 Type of CompoundSteroids
    FormulaC27H44O7Physical DescriptionPowder
    Download Manual    COA    MSDS    SDFSimilar structuralComparison (Web)
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    20-Hydroxyecdysone Description
    Source: The roots of Cyanotis arachnoidea C. B. Clarke.
    Biological Activity or Inhibitors: 1. 20-Hydroxyecdysone slowly reduces food consumption and then indirectly induces a state of starvation resulting in the elevation of the mRNA levels of InR , IRS , PI3K110 , and PDK in the Bombyx fat body during molting and pupation, and 20-hydroxyecdysone inhibits innate immunity in the fat body during Bombyx postembryonic development.
    2. 20-Hydroxyecdysone as ingredients in nutritional supplements for various sports, particularly bodybuilding.
    3. 20-Hydroxyecdysone induces autophagy and caspase activity, predominantly transduced by E93 in the remodeling fat body of Drosophila.
    4. 20-Hydroxyecdysone (via GPCR activation and calcium signaling) activates CaMKII phosphorylation and nuclear translocation, which regulate USP1 lysine acetylation to form an EcRB1-USP1 complex for 20E response gene transcription.
    5. 20-Hydroxyecdysone acts as both a positive and a negative regulator of EDG transcription, sequences in the promoter regions of two of the EDGs are similar to an ecdysone response element and may play a role in negative regulation.
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 2.0807 mL 10.4037 mL 20.8073 mL 41.6146 mL 52.0183 mL
    5 mM 0.4161 mL 2.0807 mL 4.1615 mL 8.3229 mL 10.4037 mL
    10 mM 0.2081 mL 1.0404 mL 2.0807 mL 4.1615 mL 5.2018 mL
    50 mM 0.0416 mL 0.2081 mL 0.4161 mL 0.8323 mL 1.0404 mL
    100 mM 0.0208 mL 0.104 mL 0.2081 mL 0.4161 mL 0.5202 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    20-Hydroxyecdysone References Information
    Citation [1]

    J Biol Chem. 2015 Mar 27;290(13):8469-81.

    The steroid hormone 20-hydroxyecdysone via nongenomic pathway activates Ca2+/calmodulin-dependent protein kinase II to regulate gene expression.[Pubmed: 25670853 ]
    The steroid hormone 20-Hydroxyecdysone (20E) triggers calcium signaling pathway to regulate 20-Hydroxyecdysone response gene expression, but the mechanism underlying this process remains unclear. We propose that the 20-Hydroxyecdysone-induced phosphorylation of Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) serves an important function in 20-Hydroxyecdysone response gene transcription in the lepidopteran insect Helicoverpa armigera. CaMKII showed increased expression and phosphorylation during metamorphosis. 20-Hydroxyecdysone elevated CaMKII phosphorylation. However, the G protein-coupled receptor (GPCR) and ryanodine receptor inhibitor suramin, the phospholipase C inhibitor U73122, and the inositol 1,4,5-triphosphate receptor inhibitor xestospongin C suppressed 20-Hydroxyecdysone-induced CaMKII phosphorylation. Two ecdysone-responsible GPCRs and Gαq protein were involved in 20-Hydroxyecdysone-induced CaMKII phosphorylation by RNA interference analysis. 20-Hydroxyecdysone regulated CaMKII threonine phosphorylation at amino acid 290, thereby inducing CaMKII nuclear translocation. CaMKII knockdown by dsCaMKII injection into the larvae prevented the occurrence of larval-pupal transition and suppressed 20-Hydroxyecdysone response gene expression. CaMKII phosphorylation and nuclear translocation maintained USP1 lysine acetylation at amino acid 303 by inducing histone deacetylase 3 phosphorylation and nuclear export. The lysine acetylation of USP1 was necessary for the interaction of USP1 with EcRB1 and their binding to the ecdysone response element. Results suggest that 20-Hydroxyecdysone (via GPCR activation and calcium signaling) activates CaMKII phosphorylation and nuclear translocation, which regulate USP1 lysine acetylation to form an EcRB1-USP1 complex for 20-Hydroxyecdysone response gene transcription.
    Citation [2]

    Insect Mol Biol. 2014 Aug;23(4):407-16.

    20-hydroxyecdysone mediates non-canonical regulation of mosquito vitellogenins through alternative splicing.[Pubmed: 24720618]
    We demonstrate that the hormone 20-Hydroxyecdysone (20E) is responsible for regulating post-transcriptional splicing of vitellogenin. After exposure of previtellogenic fat bodies to 20-Hydroxyecdysone, vitellogenin expression switches from a non-productive intron-retaining transcript to a spliced protein-coding transcript. This effect is independent of factors classically known to influence transcription, such as juvenile hormone-mediated competence and amino acid signalling through the target of rapamycin pathway. Non-canonical regulation of vitellogenesis through RUST is a novel role for the multifunctional hormone 20-Hydroxyecdysone, and may have important implications for general patterns of gene regulation in mosquitoes.
    Citation [3]

    Insect Biochem Mol Biol. 2014 Feb;45:30-9.

    E93 predominantly transduces 20-hydroxyecdysone signaling to induce autophagy and caspase activity in Drosophila fat body.[Pubmed: 24316411]
    Both autophagy and caspase activity are induced by a pulse of molting hormone (20-Hydroxyecdysone, 20E) via the 20E nuclear receptor complex, EcR-USP. We here demonstrate that E93, a 20-Hydroxyecdysone primary-response gene encoding an HTH transcription factor, predominantly transduces 20-Hydroxyecdysone signaling to induce autophagy and caspase activity in the remodeling fat body. Taken together, we conclude that autophagy and caspase activity are induced by 20-Hydroxyecdysone and predominantly transduced by E93 in the remodeling fat body of Drosophila.
    Citation [4]

    Insect Biochem Mol Biol. 2013 Nov;43(11):1068-78.

    Balancing crosstalk between 20-hydroxyecdysone-induced autophagy and caspase activity in the fat body during Drosophila larval-prepupal transition.[Pubmed: 24036278]
    Both autophagy and caspase activity progressively increase in the remodeling fat body, and they are induced by a pulse of the molting hormone (20-Hydroxyecdysone, 20E) during the larval-prepupal transition. Inhibition of autophagy and/or caspase activity in the remodeling fat body results in 25-40% pupal lethality, depending on the genotypes. Interestingly, a balancing crosstalk occurs between autophagy and caspase activity in this tissue: the inhibition of autophagy induces caspase activity and the inhibition of caspases induces autophagy. The Drosophila remodeling fat body provides an in vivo model for understanding the molecular mechanism of the balancing crosstalk between autophagy and caspase activity, which oppose with each other and are induced by the common stimulus 20-Hydroxyecdysone, and blockage of either path reinforces the other path.
    Citation [5]

    J Insect Physiol. 2010 Oct;56(10):1436-44.

    Transcriptional regulation of the insulin signaling pathway genes by starvation and 20-hydroxyecdysone in the Bombyx fat body.[Pubmed: 20197069 ]
    Genetic studies in the fruitfly, Drosophila melanogaster, have uncovered a conserved insulin/insulin growth factor signaling (IIS) pathway that regulates nutrition-dependent growth rates of insects. From the silkworm, Bombyx mori, we have identified and characterized several key genes involved in the IIS pathway, including InR, IRS, PI3K110, PI3K60, PTEN, PDK, and Akt. Tissue distribution analysis showed that most of these genes were highly expressed in the fat body implying that the IIS pathway is functionally important within insect adipose tissue. Developmental profile studies revealed that the expression levels of InR, IRS, PI3K110, and PDK were elevated in the fat body during molting and pupation, periods when animals ceased feeding and hemolymph levels of 20-Hydroxyecdysone (20E) were high. Starvation rapidly up-regulated the mRNA levels of these same genes in the fat body, while 20E slowly induced their transcription. We conclude that 20E slowly reduces food consumption and then indirectly induces a state of starvation resulting in the elevation of the mRNA levels of InR, IRS, PI3K110, and PDK in the Bombyx fat body during molting and pupation.
    Citation [6]

    Dev Biol. 1991 Aug;146(2):569-82.

    20-Hydroxyecdysone is required for, and negatively regulates, transcription of Drosophila pupal cuticle protein genes.[Pubmed: 1713868]
    Transcripts of ecdysone-dependent genes (EDGs) accumulate in isolated imaginal discs with 8 hr after exposure to a pulse of the steroid hormone 20-Hydroxyecdysone (20-HE; 1 microgram/ml for 6 hr) but not in discs cultured in the continuous presence or absence of the hormone. Sequence analyses show that two of the EDGs are members of gene families encoding insect cuticle proteins. We conclude that a third EDG encodes a cuticle protein because the conceptual glycine-rich protein contains sequence motifs similar to those found in insect egg shell proteins and vertebrate cytokeratins and because expression of this gene is limited to tissues that deposit the pupal cuticle. Nuclear run-on assays show that the hormone-dependent expression of each of these EDGs is due to transcriptional regulation. Readdition of hormone to imaginal discs actively synthesizing the EDG messages causes rapid repression of EDG transcription. Thus, 20-HE acts as both a positive and a negative regulator of EDG transcription. Sequences in the promoter regions of two of the EDGs are similar to an ecdysone response element and may play a role in negative regulation.