|Source:||The herbs of Ocotea cymbarum.|
|Biological Activity or Inhibitors:||1. Burchellin has larvicidal activity, it can interfer with the development cycle of the mosquito, where its strongest toxic effect is 100% mortality in larvae (L3) at concentrations ≥ 30 ppm.
2. Burchellin and licarin A have activity against Trypanosoma cruzi, they can induce trypomastigote death with IC(50)/24 h of 520 microM and 960 microM, respectively.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.9377 mL||14.6886 mL||29.3772 mL||58.7544 mL||73.443 mL|
|5 mM||0.5875 mL||2.9377 mL||5.8754 mL||11.7509 mL||14.6886 mL|
|10 mM||0.2938 mL||1.4689 mL||2.9377 mL||5.8754 mL||7.3443 mL|
|50 mM||0.0588 mL||0.2938 mL||0.5875 mL||1.1751 mL||1.4689 mL|
|100 mM||0.0294 mL||0.1469 mL||0.2938 mL||0.5875 mL||0.7344 mL|
Parasit Vectors. 2014 Apr 8;7:172.
|Burchellin: study of bioactivity against Aedes aegypti.[Pubmed: 24713267]|
|The data showed that Burchellin interfered with the development cycle of the mosquito, where its strongest toxic effect was 100% mortality in larvae (L3) at concentrations ≥ 30 ppm. This compound did not show target cell toxicity in peritoneal macrophages from BALB/c mice, and proved to have molecular stability when dissolved in water. The L3 and L4 larvae treated with the compound showed cellular destruction and disorganization, cell spacing, and vacuolization of epithelial cells in small regions of the midgut.CONCLUSION:The neolignan Burchellin proved to be a strong candidate for a natural, safe and stable phytolarvicidal to be used in population control of A. aegypti.|
Exp Parasitol. 2010 Mar;124(3):319-24.
|Neolignans from plants in northeastern Brazil (Lauraceae) with activity against Trypanosoma cruzi.[Pubmed: 19944690 ]|
|Trypanosoma cruzi is the ethiological agent for Chagas disease in Latin America. This study aimed to test the trypanocidal effect of licarin A and Burchellin isolated from plants in northeastern Brazil. These neolignans were tested on T. cruzi and on peritoneal macrophages, to evaluate drug toxicity. Epimastigote growth was inhibited in 45% with licarin A and 20% with Burchellin with an IC(50)/96 h of 462.7 microM and 756 microM, respectively. Epimastigotes treated with licarin A presented swollen mitochondria and disorganized mitochondrial cristae, kDNA and Golgi complex. When treated with Burchellin, they presented enormous autophagosomes and chromatin disorganization. Licarin A and Burchellin were able to induce trypomastigote death with IC(50)/24 h of 960 microM and 520 microM, respectively. Although licarin A presented an IC(50) for trypomastigotes higher than for epimastigotes, both substances acted as therapeutic trypanocidal agents, because they were able to kill parasites without affecting macrophages. Due to our results, Burchellin and licarin A need to be further analysed to observe if they may be used as alternative blood additive prophylaxis against Chagas disease, since it has been established that blood transfusion is an important mechanism in the transmission process.|