ChemFaces is a professional high-purity natural products manufacturer.
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More articles cited ChemFaces products.
J Ethnopharmacol.23 February 2017Molecules. 2018 Jan 24;Food Analytical Methods07 April 2017J Pharm Biomed Anal. 2016 Jun 25;129:50-59. Sci Rep. 2017 Oct 11;
Evid Based Complement Alternat Med. 2017:1583185Sci Rep. 2017 Apr 11;J Chromatogr Sci.2015 Feb 5. pii: bmu231. Sci Rep. 2017 Dec 11;J. Agric. Food Chem.,2015, 63 (44), 9869–9878
Phytomedicine.2018 Jan 1;Free Radic Biol Med.2017 Nov;J Ethnopharmacol.2016 Jun 25. J Ethnopharmacol. 2017 Feb 23;
Our products had been exported to the following research institutions and universities, And still growing.
Northeast Normal University Chan... (China)Florida International University (USA)Julius Kühn-Institut (Germany)Warszawski Uniwersytet Medyczny (Poland)
University of Minnesota (USA)Universidad de Ciencias y Artes ... (Mexico)Chiang Mai University (Thailand)University of South Australia (Australia)
Almansora University (Egypt)Rio de Janeiro State University (Brazil)Uniwersytet Gdański (Poland)
||Juglone has anti-inflammatory, and anti-cancer activities, it can significantly inhibit the proliferation and induce the apoptosis of SiHa cells and Caski cells; it prevents high-fat diet-induced liver injury and nerve inflammation in mice through inhibition of inflammatory cytokine secretion, NF-kappa B activation and endotoxin production. Juglone stimulates suicidal erythrocyte death or eryptosis at least in part by upregulation of ceramide abundance, energy depletion and activation of PKC.
||Calcium Channel | PKC | TLR | NF-kB | TNF-α | IL Receptor | Bcl-2/Bax|
|Basic Clin Pharmacol Toxicol. 2015 Jun;116(6):460-7. |
|Enhanced eryptosis following juglone exposure.[Pubmed: 25348830 ]|
|Juglone, a quinone isolated from Juglans mandshurica Maxim, has previously been shown to be effective against malignancy. The effect is at least partially due to stimulation of suicidal death or apoptosis of tumour cells. On the other hand, Juglone has been shown to counteract apoptosis, for example, of neurons. In analogy to apoptosis of nucleated cells, erythrocytes may enter eryptosis, a suicidal death characterized by cell shrinkage and breakdown of phosphatidylserine asymmetry of the cell membrane with phosphatidylserine exposure at the erythrocyte surface. Stimulators of eryptosis include increase in cytosolic Ca(2+) activity [(Ca(2+) )i ]. |
METHODS AND RESULTS:
This study explored whether Juglone stimulates eryptosis. To this end, erythrocyte volume was estimated from forward scatter, phosphatidylserine exposure at the erythrocyte surface from FITC annexin V binding, ceramide abundance from binding of fluorescent antibodies in flow cytometry and cytosolic ATP with a luciferin-luciferase-based assay. As a result, a 24-hr exposure of human erythrocytes to Juglone (5 μM) significantly decreased erythrocyte forward scatter. Juglone (1-5 μM) significantly increased the percentage of annexin V binding cells. Juglone (5 μM) significantly increased ceramide abundance at the erythrocyte surface and decreased erythrocyte ATP concentration. The effect of Juglone (10 μM) on annexin V binding was slightly but significantly blunted by removal of extracellular Ca(2+) and by addition of protein kinase C (PKC) inhibitor staurosporine (1 μM).
In conclusion, Juglone stimulates suicidal erythrocyte death or eryptosis at least in part by upregulation of ceramide abundance, energy depletion and activation of PKC.
|Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Feb;31(2):186-9. |
|Juglone inhibits proliferation and induces apoptosis of human cervical squamous cancer SiHa cells[Pubmed: 25652859]|
|To explore the effect of Juglone on proliferation and apoptosis of human cervical squamous cancer SiHa cells. |
METHODS AND RESULTS:
Cultured SiHa cells in the exponential growth phase were grouped into blank control group and 10, 20, 50, 80 and 100 μmol/L Juglone treatment groups. Methyl thiazolyl tetrazolium (MTT) assay was adopted to observe the inhibitory effect of Juglone on the proliferation of SiHa cells, and then 50% inhibitory concentration (IC50) was calculated through formula. Annexin V-FITC/PI double staining and flow cytometry were used to detect the effect of 20 μmol/L Juglone on SiHa cell apoptosis. Western blot was applied to determine the expressions of Bcl-2 and Bax. MTT assay showed that, compared with the control group, treatment groups all showed significant inhibitory effects on SiHa cell growth, and IC50 was 20.4 μmol/L. Flow cytometry demonstrated that early apoptosis rate of SiHa cells in the control group was (2.46 ± 0.37)%, and after treatment with 20 μmol/L Juglone for 12 hours, the apoptosis rate was raised to (18.47 ± 2.26)%; Western blot analysis showed that the expression of Bcl-2 decreased while the expression of Bax increased significantly in SiHa cells treated with 20 μmol/L Juglone.
Juglone could significantly inhibit the proliferation and induce the apoptosis of SiHa cells.
|Biochem Biophys Res Commun. 2015 Jul 3;462(3):245-50. |
|Juglone prevents metabolic endotoxemia-induced hepatitis and neuroinflammation via suppressing TLR4/NF-κB signaling pathway in high-fat diet rats.[Pubmed: 25964086]|
|Juglone as a natural production mainly extracted from green walnut husks of Juglans mandshurica has been defined as the functional composition among a series of compounds. It showed powerful protective effect in various diseases by inhibiting inflammation and tumor cells growth. However, studies on its anti-inflammatory effect based on high-fat diet-induced hepatitis and neuroinflammation are still not available. |
METHODS AND RESULTS:
In this regard, we first investigated whether Juglone suppresses high-fat diet-stimulated liver injury, hypothalamus inflammation and underlying mechanisms by which they may recover them. SD rats were orally treated with or without high-fat diet, 0.25 mg/kg or 1 mg/kg Juglone for 70 days. Subsequently, blood, hypothalamus and liver tissue were collected for different analysis. Also, the primary astrocytes were isolated and used to analyze the inhibitory effect of Juglone in vitro. Analysis of inflammatory cytokines declared that the inhibition of TNF-α, IL-1β and IL-6 could be carried by Juglone in response to high-fat diet rats. Meanwhile, TLR4 expression and NF-kappa activity also have been confirmed to be the key link in the development of hepatitis and nerve inflammation. The activation was significantly suppressed in treatment group as compared with model.
These results indicated that Juglone prevents high-fat diet-induced liver injury and nerve inflammation in mice through inhibition of inflammatory cytokine secretion, NF-kappa B activation and endotoxin production.
||The barks of Juglans regia L.
||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Recent ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.PMID: 29328914
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.PMID: 29149595
Scientific Reports 2017 Dec 11;7(1):17332.doi: 10.1038/s41598-017-17427-6.PMID: 29230013
Molecules. 2017 Oct 27;22(11). pii: E1829.doi: 10.3390/molecules22111829.PMID: 29077044
J Cell Biochem. 2018 Feb;119(2):2231-2239.doi: 10.1002/jcb.26385. PMID: 28857247
Phytomedicine. 2018 Feb 1;40:37-47. doi:10.1016/j.phymed.2017.12.030PMID: 29496173
Calculate Dilution Ratios(Only for Reference)
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
|Wei Sheng Yan Jiu. 2014 Nov;43(6):959-61, 971. |
|Proliferation inhibition and apoptosis induction of Juglone on human cervical cancer Caski cells[Pubmed: 25603606]|
|To explore the effects of Juglone on proliferation and apoptosis of human cervical cancer Caski cells, and to further study the related mechanism of cell apoptosis. |
METHODS AND RESULTS:
Cultured Caski cells were incubated with 20, 40, 60, 80 and 100 μmol/L Juglone for 24 h. The proliferation of Caski cells was detected by methyl thiazolyl tetrazolium (MTT) assay. The cell apoptosis were detected by transmission electron microscope. The expression of Bcl-2 and Bax were detected by Western blot. MTT results showed that in different doses of Juglone groups, the Caski cell growth was greatly inhibited (P < 0.05, P < 0.01) and showed dose dependent when compared with control group except 20 μmol/L. The IC50 of Juglone was 42.4 μmol/L. After treatment on Caski cells with 40 μmol/L Juglone, typical apoptosis characteristics was observed by transmission electronmicro scope. The expression of Bcl-2 was decreased while the expression of Bax was increased significantly when compared with control group (P < 0.05).
Juglone significantly inhibits the proliferation and induces the apoptosis of Caski cells in vitro.