ChemFaces is a professional high-purity natural products manufacturer.
Product Intended Use
1. Reference standards
2. Pharmacological research
How to Order
Orders via your E-mail:
1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Sent to Email: email@example.com
Order & Inquiry & Tech Support
Address: No. 83, CheCheng Rd., WETDZ, Wuhan, Hubei 430056, PRC
Delivery & Payment method
1. Usually delivery time: Next day delivery by 9:00 a.m. Order now
2. We accept: Wire transfer & Credit card & Paypal & Western Union
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
More articles cited ChemFaces products.
Polytechnic University of Catalonia2016J. Agric. Food Chem.,2015, 63 (44), 9869–9878Ind Crops Prod.2014 Dec 1;62:173-178.J Pharm Biomed Anal. 2016 Jun 25;129:50-59. Acta ChromatographicaOct. 11, 2016
Phytomedicine1 Feb. 2018;Front Pharmacol. 2017 Sep 29;Front Plant Sci. 2017 May 8;Acta Biochim Pol.2015 May 26J Agric Food Chem.2016 Sep 7
Curr Eye Res.2018 Jan;University of Limpopo2016Sci Rep. 2017 Jan 9;J Agric Food Chem.2018 Jan 10;
Our products had been exported to the following research institutions and universities, And still growing.
Universiti Malaysia Pahang (Malaysia)St. Jude Children Research Hospi... (USA)Regional Crop Research Institute (Korea)VIB Department of Plant Systems ... (Belgium)
University of Amsterdam (Netherlands)CSIRO - Agriculture Flagship (Australia)University of Bonn (Germany)University of Indonesia (Indonesia)
Universidad de Buenos Aires (Argentina)Sri Sai Aditya Institute of Phar... (India)Wroclaw Medical University (Poland)
||Theobromine is a xanthine alkaloid widely consumed as stimulants and snacks in coffee and cocoa based foods, it may form the basis for a new class of antitussive drugs. Theobromine has antioxidants and also capable of prooxidant action, it may have therapeutic potential for diabetic nephropathy, by reducing kidney ECM accumulation in diabetic. It can inhibit adenosine receptor A1 (AR1) signaling.|
||PARP | AMPK | TGF-β/Smad | LDL|
|Med Sci Monit. 2003 Sep;9(9):BR325-30. |
|Antioxidant and prooxidant properties of caffeine, theobromine and xanthine.[Pubmed: 12960921]|
|Caffeine, along with its catabolic products Theobromine and xanthine, is a key component of tea and coffee. These compounds are structurally similar to uric acid, a known antioxidant which is present in blood at relatively high concentrations, but also shows prooxidant activity.
In view of the structural similarity between uric acid and caffeine and its metabolites, we studied the antioxidant and prooxidant properties of these compounds.
METHODS AND RESULTS:
Antioxidant activity was determined by measuring the quenching effect of the compounds on oxidative DNA degradation by a hydroxyl radical generating system. Prooxidant activity was studied by measuring the ability of the compounds to oxidatively degrade DNA in the presence of copper ions. Caffeine, Theobromine and xanthine have a quenching effect on the production of hydroxyl radicals, as well as on oxidative DNA breakage by hydroxyl radicals. Consistent with previous observations that many known antioxidants of plant origin are also capable of prooxidant action, the purine alkaloids also show oxidative DNA breakage in the presence of transition metal ions.
The alkaloid caffeine and its catabolic products Theobromine and xanthine exhibit both antioxidant and prooxidant properties.
The results lead to the observation that caffeine and its metabolites may also contribute to the overall antioxidant and chemopreventive properties of caffeine-bearing beverages, such as tea.
|FASEB J. 2005 Feb;19(2):231-3. |
|Theobromine inhibits sensory nerve activation and cough.[Pubmed: 15548587 ]|
|Cough is a common and protective reflex, but persistent coughing is debilitating and impairs quality of life. Antitussive treatment using opioids is limited by unacceptable side effects, and there is a great need for more effective remedies.
METHODS AND RESULTS:
The present study demonstrates that Theobromine, a methylxanthine derivative present in cocoa, effectively inhibits citric acid-induced cough in guinea-pigs in vivo. Furthermore, in a randomized, double-blind, placebo-controlled study in man, Theobromine suppresses capsaicin-induced cough with no adverse effects. We also demonstrate that Theobromine directly inhibits capsaicin-induced sensory nerve depolarization of guinea-pig and human vagus nerve suggestive of an inhibitory effect on afferent nerve activation.
These data indicate the actions of Theobromine appear to be peripherally mediated. We conclude Theobromine is a novel and promising treatment, which may form the basis for a new class of antitussive drugs.
|Am J Clin Nutr. 2013 Jun;97(6):1201-9. |
|Effect of cocoa and theobromine consumption on serum HDL-cholesterol concentrations: a randomized controlled trial.[Pubmed: 23595874]|
| Evidence from clinical studies has suggested that cocoa may increase high-density lipoprotein (HDL)-cholesterol concentrations. However, it is unclear whether this effect is attributable to flavonoids or Theobromine, both of which are major cocoa components.
METHODS AND RESULTS:
We investigated whether pure Theobromine increases serum HDL cholesterol and whether there is an interaction effect between Theobromine and cocoa. DESIGN: The study had a 2-center, double-blind, randomized, placebo-controlled, full factorial parallel design. After a 2-wk run-in period, 152 healthy men and women (aged 40-70 y) were randomly allocated to consume one 200-mL drink/d for 4 wk that contained 1) cocoa, which naturally provided 150 mg Theobromine and 325 mg flavonoids [cocoa intervention (CC)], 2) 850 mg pure Theobromine [Theobromine intervention (TB)], 3) cocoa and added Theobromine, which provided 1000 mg Theobromine and 325 mg flavonoids [Theobromine and cocoa intervention (TB+CC)], or 4) neither cocoa nor Theobromine (placebo). Blood lipids and apolipoproteins were measured at the start and end of interventions. RESULTS: In a 2-factor analysis, there was a significant main effect of the TB (P < 0.0001) but not CC (P = 0.1288) on HDL cholesterol but no significant interaction (P = 0.3735). The TB increased HDL-cholesterol concentrations by 0.16 mmol/L (P < 0.0001). Furthermore, there was a significant main effect of the TB on increasing apolipoprotein A-I (P < 0.0001) and decreasing apolipoprotein B and LDL-cholesterol concentrations (P < 0.02).
Theobromine independently increased serum HDL-cholesterol concentrations by 0.16 mmol/L. The lack of significant cocoa and interaction effects suggested that Theobromine may be the main ingredient responsible for the HDL cholesterol-raising effect.
||The fruits of Theobroma cacao L.
||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Recent ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.PMID: 29328914
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.PMID: 29149595
Scientific Reports 2017 Dec 11;7(1):17332.doi: 10.1038/s41598-017-17427-6.PMID: 29230013
Molecules. 2017 Oct 27;22(11). pii: E1829.doi: 10.3390/molecules22111829.PMID: 29077044
J Cell Biochem. 2018 Feb;119(2):2231-2239.doi: 10.1002/jcb.26385. PMID: 28857247
Phytomedicine. 2018 Feb 1;40:37-47. doi: 10.1016/j.phymed.2017.12.030.PMID: 29496173
Calculate Dilution Ratios(Only for Reference)
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
|Am J Physiol Renal Physiol. 2015 Feb 1;308(3):F209-25. |
|Theobromine increases NAD⁺/Sirt-1 activity and protects the kidney under diabetic conditions.[Pubmed: 25411384]|
|Reduction in sirtuin 1 (Sirt-1) is associated with extracellular matrix (ECM) accumulation in the diabetic kidney. Theobromine may reduce kidney ECM accumulation in diabetic rats.
METHODS AND RESULTS:
In the current study, we aimed to unravel, under diabetic conditions, the mechanism of kidney ECM accumulation induced by a reduction in Sirt-1 and the effect of Theobromine in these events. In vitro, we used immortalized human mesangial cells (iHMCs) exposed to high glucose (HG; 30 mM), with or without small interfering RNA for NOX4 and Sirt-1. In vivo, spontaneously hypertensive rats (SHR) were rendered diabetic by means of streptozotocin and studied after 12 wk. The effects of treatment with Theobromine were investigated under both conditions. HG leads to a decrease in Sirt-1 activity and NAD(+) levels in iHMCs. Sirt-1 activity could be reestablished by treatment with NAD(+), silencing NOX4, and poly (ADP-ribose) polymerase-1 (PARP-1) blockade, or with Theobromine. HG also leads to a low AMP/ATP ratio, acetylation of SMAD3, and increased collagen IV, which is prevented by Theobromine. Sirt-1 or AMPK blockade abolished these effects of Theobromine. In diabetic SHR, Theobromine prevented increases in albuminuria and kidney collagen IV, reduced AMPK, elevated NADPH oxidase activity and PARP-1, and reduced NAD(+) levels and Sirt-1 activity.
These results suggest that in diabetes mellitus, Sirt-1 activity is reduced by PARP-1 activation and NAD(+) depletion due to low AMPK, which increases NOX4 expression, leading to ECM accumulation mediated by transforming growth factor (TGF)-β1 signaling. It is suggested that Sirt-1 activation by Theobromine may have therapeutic potential for diabetic nephropathy.
|Mutat Res. 1975;32(2):169-78. |
|Theobromine and theophylline.[Pubmed: 765793]|
|Theobromine and theophylline have a limited therapeutic use and in addition they occur in plants used in the preparation of a number of widely consumed drinks. Thus most of the population must be exposed to both compounds.
METHODS AND RESULTS:
Chromosome abnormalities are caused by both Theobromine and theophylline in plant cells and in mammalian cells in culture, and both have anti-mitotic activity. While they are fairly potent mutagens in Escherichia coli and other lower organisms the rather scanty available evidence suggests that they are not mutagenic in mammals. The difference in mutagenic activity may be due to the reported inability of E. coli to demethylate these compounds, a process which occurs readily in mammals including man.
The structure-activity relationships of these compounds are complex but the available evidence suggests that methylation at position 1 is the most important for both mutagenic activity and the anti-mitotic effect while methylation at position 3 is of most importance in the action on chromosomes.