|Source:||The seed shell of Hevea brasiliensis.|
|Biological Activity or Inhibitors:||1. erythro-Guaiacylglycerol-β-O-4'-dehydrodisinapyl ether shows inhibitory activity against human leukocyte elastase with the IC50 value of 171 umol·L-1.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.7105 mL||8.5527 mL||17.1054 mL||34.2108 mL||42.7636 mL|
|5 mM||0.3421 mL||1.7105 mL||3.4211 mL||6.8422 mL||8.5527 mL|
|10 mM||0.1711 mL||0.8553 mL||1.7105 mL||3.4211 mL||4.2764 mL|
|50 mM||0.0342 mL||0.1711 mL||0.3421 mL||0.6842 mL||0.8553 mL|
|100 mM||0.0171 mL||0.0855 mL||0.1711 mL||0.3421 mL||0.4276 mL|
Chinese Journal of New Drugs, 2012, 21(19):2311-2315.
|Chemical constituents of the seed shell of Hevea brasiliensis.[Reference: WebLink]|
|To study the chemical constituents of the seed shell of Hevea brasiliensis. Methods: The compounds were isolated by chromatography on silica gel, LH-20, ODS-C18 and HPLC. Their structures were identified by physicochemical and spectral analysis. MTT assays in vitro were used to explore the inhibitory effect of these compounds on B16 cancer cells, and a high through-put screening method was used to explore the HLE inhibitors. Results: Nine compounds were isolated and identified as vanillin(1), coniferaldehyde(2), erythro-guaiacylglycerol-β-coniferyl aldehyde ether(3), threo-guaiacylglycerol-β-coniferyl aldehyde ether(4), (-)-balanophonin(5), isoamericanol A (6), americanol A (7),erythro-Guaiacylglycerol-beta-O-4'-dehydrodisinapyl ether (8), and buddlenol A (9). Compound 9 showed anti-tumor activity against B16 cancer cells with IC50 value of 20.6 μmol·L-1. Compounds 5~9 showed inhibitory activity against human leukocyte elastase with IC50 values of 168, 45.5, 57.6, 171 and 115 μmol·L-1, respectively. Conclusion: All compounds were isolated from this plant for the first time. Compound 9 showed inhibitory activity on B16 cancer cells, and compounds 5~9 were reported as human leukocyte elastase inhibitors for the first time.|