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Buxbodine B
Buxbodine B
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Buxbodine B
Price:
CAS No.: 390362-51-3
Catalog No.: CFN98626
Molecular Formula: C26H41NO2
Molecular Weight: 399.6 g/mol
Purity: >=98%
Type of Compound: Alkaloids
Physical Desc.: Solid
Source: The herbs of Buxus bodinieri Levl.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF
Similar structural: Comparison (Web)  (SDF)
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According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
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Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Buxbodine B has weak anti-AChE activity with IC50 values in the range of 10.8-98μM.
Targets: AChR
In vitro:
Steroids. 2015 Mar;95:73-9.
Bioactive steroidal alkaloids from Buxus macowanii Oliv.[Pubmed: 25528196]

METHODS AND RESULTS:
Chemical investigation of the crude methanolic extract of Buxus macowanii resulted in the isolation of five new steroidal alkaloids, 31-hydroxybuxatrienone (1), macowanioxazine (2), 16α-hydroxymacowanitriene (3), macowanitriene (4), macowamine (5), along with five known steroidal bases, Nb-demethylpapillotrienine (6), moenjodaramine (7), irehine (8), Buxbodine B (9) and buxmicrophylline C (10). Structures of compounds 1-10 were elucidated with the aid of spectroscopic methods including 1D and 2D NMR techniques and mass spectrometry. Compounds 1, 3, and 4 belong to a rare class of Buxus alkaloids having Δ(1,2) 9(10→19) abeo triene system.
CONCLUSIONS:
All isolates were evaluated for in vitro acetylcholinesterase (AChE) inhibitory activity and found to exhibit moderate to weak anti-AChE activity with IC50 values in the range of 10.8-98μM. Compounds 1 and 6 were also moderately active in BACE1 inhibitory assay.
Buxbodine B Description
Source: The herbs of Buxus bodinieri Levl.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.5025 mL 12.5125 mL 25.025 mL 50.0501 mL 62.5626 mL
5 mM 0.5005 mL 2.5025 mL 5.005 mL 10.01 mL 12.5125 mL
10 mM 0.2503 mL 1.2513 mL 2.5025 mL 5.005 mL 6.2563 mL
50 mM 0.0501 mL 0.2503 mL 0.5005 mL 1.001 mL 1.2513 mL
100 mM 0.025 mL 0.1251 mL 0.2503 mL 0.5005 mL 0.6256 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Structure Identification:
Acta Botanica Yunnanica, 2001,23(3):357-62.
Steroidal Alkaloids from Buxus bodinieri[Reference: WebLink]
Five new steroidal alkaloids have been isolated from Buxus bodinieri Lévl.,a common ornamental in South-China.
METHODS AND RESULTS:
On the basis of IR, UV, MS, NMR spectral analyses and chemical evidence, the chemical structure of these new steroidal alkaloids were deduced as: buxbodine A (1)(20α- dimethylamino-9β,19-cyclo-4,4,14α-trimethy-5α-pregnane); Buxbodine B (2)(16β-hydroxy-20α-dimethylamino-9β,19-cyclo-4,4,14α-trimethy-5α-pregn-1(2)-en-3-one); buxbodine C (3)(3β-dimethylamino-9β,19-cyclo-4,4,14α-trimethy-5α-pregn-6(7), 17 (20)-dien-16-one), buxbodine D (4)(3β-dimethylamino-20α-acetoxylamino-9β,19-cyclo -4,4,14α-trimethy-5α-pregn-6(7)-ene) and buxbodine E (5)(3(-dimethylamino-20α-amino -9β,19-cyclo-4,4,14α-trimethy-5α-pregn-6(7)-ene), respectively.
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