Science | Nature | Cell | View More
Natural Products
Isokaempferide
Isokaempferide
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Isokaempferide
Price: $368 / 5mg
CAS No.: 1592-70-7
Catalog No.: CFN93002
Molecular Formula: C16H12O6
Molecular Weight: 300.3 g/mol
Purity: >=98%
Type of Compound: Flavonoids
Physical Desc.: Yellow powder
Source: The herbs of Geranium wilfordii Maxim
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF
Similar structural: Comparison
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $312.8 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • Shanghai Institute of Organic C... (China)
  • Heinrich-Heine-University Düss... (Germany)
  • Pennsylvania State University (USA)
  • University of Hawaii Cancer Center (USA)
  • VIB Department of Plant Systems... (Belgium)
  • Indian Institute of Science (India)
  • Universidade do Porto (Portugal)
  • University of East Anglia (United Kingdom)
  • University of Bordeaux (France)
  • Technical University of Denmark (Denmark)
  • The Australian National University (Australia)
  • More...
Package
Featured Products
Citral

Catalog No: CFN70135
CAS No: 5392-40-5
Price: $30/20mg
Robinin

Catalog No: CFN98375
CAS No: 301-19-9
Price: $318/5mg
Daucosterol

Catalog No: CFN98713
CAS No: 474-58-8
Price: $80/20mg
Poricoic acid A(F)

Catalog No: CFN92838
CAS No: 137551-38-3
Price: $218/20mg
Mangiferin

Catalog No: CFN98719
CAS No: 4773-96-0
Price: $40/20mg
Neomangiferin

Catalog No: CFN98122
CAS No: 64809-67-2
Price: $80/20mg
Procyanidin B2

Catalog No: CFN99558
CAS No: 29106-49-8
Price: $138/20mg
Terpineol

Catalog No: CFN92688
CAS No: 98-55-5
Price: $30/20mg
Decursin

Catalog No: CFN98509
CAS No: 5928-25-6
Price: $138/20mg
Quercitrin

Catalog No: CFN98850
CAS No: 522-12-3
Price: $40/20mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Isokaempferide shows hepatoprotective, antiproliferative, and anti-inflammatory effects, the anti-inflammatory effects can be explained, at least in part, by reducing neutrophil degranulation, myeloperoxidase activity, mediators as well as TNF-alpha secretion. Isokaempferide is used as a bronchodilator, can induce relaxation of guinea-pig isolated trachea.
Targets: TNF-α | PGE | Calcium Channel | ATPase | Potassium Channel
In vitro:
Biol Pharm Bull. 2000 Apr;23(4):456-60.
Hepatoprotective effect of Combretum quadrangulare and its constituents.[Pubmed: 10784427]
The MeOH extract of leaves of Combretum quadrangulare showed significant hepatoprotective effect on D-galactosamine (D-GalN)/lipopolysaccharide (LPS)-induced experimental liver injury in mice and on D-GalN/tumor necrosis factor-alpha (TNF-alpha)-induced cell death in primary cultured mouse hepatocytes.
METHODS AND RESULTS:
Phytochemical investigation led to the isolation of thirty cycloartane-type triterpenes together with betulinic acid, beta-sitosterol, beta-sitosterol glucoside, 4 flavones (34-37), and 3 flavone C-glucosides (38-40). These compounds showed various potencies of hepatoprotective effect on D-GalN/TNF-alpha-induced cell death in primary cultured mouse hepatocytes. Quadrangularol B (29), methyl quadrangularate I (33), kamatakenin (34), 5,7,4'-trihydroxy-3,3'-dimethoxyflavone (35), 5,4'-dihydroxy-3,7,3'-trimethoxyflavone (36) and Isokaempferide (37) showed strong inhibitory effect on TNF-alpha-induced cell death with IC50 values of 34.3, 33.7, 13.3, 22.4, 13.4 and 22.8 microM, respectively, whereas clinically-used silibinin had an IC50 value of 39.6 microM and glycyrrhizin showed very weak inhibitory effect. Methyl quadrangularates A (30) and N (32), norquadrangularic acid B (31) and vitexin (40) also showed potent inhibition on TNF-alpha-induced cell death with IC50 values of 45.7, 89.3, 67.6 and 40.1 microM, respectively.
CONCLUSIONS:
The flavonoids and some of the cycloartane-type triterpenes appeared to be the hepatoprotective principles of the leaves of C. quadrangulare.
Phytother Res. 2012 Jul;26(7):1023-8.
Antiproliferative activity of flavonoids: influence of the sequential methoxylation state of the flavonoid structure.[Pubmed: 22184071]
Dracocephalum kotschyi Boiss. has been used as part of an ethnobotanical remedy against many forms of human cancer in Iran. It has been demonstrated that a flavonoid named xanthomicrol from D. kotschyi contributes to its preferential antiproliferative activity against malignant cells.
METHODS AND RESULTS:
In the present study, the antiproliferative activity of its flavonoid fraction was further characterized. Using liquid-liquid extraction and a semi-preparative reversed-phase HPLC method, eight flavonoid aglycones were isolated from the aerial parts of the plant and their identities were confirmed through MS and NMR analyses as luteolin, naringenin, apigenin, Isokaempferide, cirsimaritin, penduletin, xanthomicrol and calycopterin. The in vitro antiproliferative activity of each compound was evaluated against a panel of established normal and malignant cell lines using the MTT assay and some structure-activity relationships were observed. The hydroxyflavones (luteolin, apigenin and Isokaempferide) exerted comparable antiproliferative activities against malignant and normal cells, while the methoxylated hydroxyflavones (cirsimaritin, penduletin, xanthomicrol and calycopterin) showed preferential activities against tumor cells.
CONCLUSIONS:
This activity may be of value in treating tumors as it would exert few side effects in normal tissues. Xanthomicrol selectively inhibited the growth of human gastric adenocarcinoma, while calycopterin selectively prevented human acute promyelocytic leukemia and human colon carcinoma cells proliferation.
In vivo:
Basic Clin Pharmacol Toxicol. 2009 Mar;104(3):198-205.
Effects of amburoside A and isokaempferide, polyphenols from Amburana cearensis, on rodent inflammatory processes and myeloperoxidase activity in human neutrophils.[Pubmed: 19053991]
The present study evaluated the anti-inflammatory activity of amburoside A (a phenol glucoside) and Isokaempferide (a flavonol) isolated from the trunk bark of Amburana cearensis, a medicinal plant used in northeast Brazil for the treatment of asthma.
METHODS AND RESULTS:
Animals (male Wistar rats or Swiss mice) pre-treated with amburoside A (25 and 50 mg/kg) or Isokaempferide (12.5, 25 and 50 mg/kg), orally or intraperitoneally, showed a significant inhibition of the paw oedema induced by carrageenan (1%), prostaglandin E(2) (30 nmol/paw), histamine (200 microg/paw) or serotonin (200 microg/paw). Histological and morphometric evaluations of the rat paw oedema induced by carrageenan showed that amburoside A and Isokaempferide also inhibited the accumulation of inflammatory cells. Amburoside A reduced significantly the paw oedema and the increase in vascular permeability induced by dextran, as related to the control group. Similar results were observed with the Isokaempferide pre-treatment. Furthermore, amburoside A or Isokaempferide inhibited both leucocyte and neutrophil migrations, in mouse peritoneal cavity, after the carrageenan injection. The polyphenols were not cytotoxic and blocked N-formyl-methyl-leucyl-phenylalanine-induced myeloperoxidase release and activity in human neutrophils. In addition, amburoside A and Isokaempferide at 50 and 100 microg/ml concentrations reduced significantly the lipopolysaccharide-mediated increase in tumour necrosis factor-alpha (TNF-alpha) levels.
CONCLUSIONS:
These results provide, for the first time, evidence to support the anti-inflammatory activity of amburoside A and Isokaempferide that seems to be related to an inhibition of inflammatory mediators, such as TNF-alpha, as well as histamine, serotonin and prostaglandin E(2), besides leucocyte infiltration in a dose- or concentration-dependent manner. These anti-inflammatory effects can be explained, at least in part, by the ability of these compounds to reduce neutrophil degranulation, myeloperoxidase activity, mediators as well as TNF-alpha secretion.
Isokaempferide Description
Source: The herbs of Geranium wilfordii Maxim
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
6alpha-Hydroxydehydropachymic acid

Catalog No: CFN95412
CAS No: 176390-67-3
Price: $413/5mg
New compound 21

Catalog No: CFN95560
CAS No: N/A
Price: $413/5mg
Dihydrophaseic acid 4'-O-beta-D-gl...

Catalog No: CFN95393
CAS No: 78914-56-4
Price: $318/5mg
Glaucoside C

Catalog No: CFN95300
CAS No: 81474-89-7
Price: $318/5mg
Isosecotanapartholide

Catalog No: CFN95471
CAS No: 102926-01-2
Price: $318/10mg
(1E)-3-methoxy-8,12-epoxygermacra-...

Catalog No: CFN95219
CAS No: 1393342-06-7
Price: $413/5mg
Hosenkoside D

Catalog No: CFN95521
CAS No: 156823-94-8
Price: $318/5mg
Platycodin J

Catalog No: CFN95395
CAS No: 1325614-80-9
Price: $318/5mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 3.33 mL 16.65 mL 33.3 mL 66.6001 mL 83.2501 mL
5 mM 0.666 mL 3.33 mL 6.66 mL 13.32 mL 16.65 mL
10 mM 0.333 mL 1.665 mL 3.33 mL 6.66 mL 8.325 mL
50 mM 0.0666 mL 0.333 mL 0.666 mL 1.332 mL 1.665 mL
100 mM 0.0333 mL 0.1665 mL 0.333 mL 0.666 mL 0.8325 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Kinase Assay:
Life Sci. 2006 May 30;79(1):98-104.
Mechanisms underlying the relaxation induced by isokaempferide from Amburana cearensis in the guinea-pig isolated trachea.[Pubmed: 16455108 ]
The present study examines possible mechanisms by which the flavonoid Isokaempferide (IKPF; 5,7,4'-trihydroxy-3-methoxyflavone) from Amburana cearensis, a Brazilian medicinal plant popularly used as bronchodilator, induces relaxation of guinea-pig isolated trachea.
METHODS AND RESULTS:
In the trachea (with intact epithelium) contracted by carbachol, Isokaempferide (1-1000 microM) caused a graded relaxation, and the epithelium removal increased the sensitivity of the airway smooth muscle to Isokaempferide (EC50, in intact tissue: 77.4 [54.8-109.2] microM; in denuded epithelium: 15.0 [11.3-20.1] microM). The Isokaempferide-induced relaxation was inhibited in 41% by the nitric oxide (NO) synthase inhibitor L-NAME (100 microM); in 31% and 50% by the soluble guanylate cyclase (sGC) inhibitor ODQ (3 and 33 microM); by propranolol (31%) and also by capsaicin (37%). In the trachea pre-contracted by 40 mM KCl the pre-incubation with glibenclamide (33 microM) or iberiotoxin (IbTX, 0.1 microM), selective K(+) channel inhibitors, inhibited the Isokaempferide-induced relaxation by 39% and 38%, respectively. On the other hand, 4-aminopyridine (100 microM), a nonselective K(+) channel antagonist, did not significantly influence the effect of Isokaempferide, while IbTX induced a rightward displacement of the Isokaempferide concentration-response curve. However, in muscle pre-contracted with 120 mM KCl the relaxant effect of Isokaempferide was significantly reduced and not affected by glibenclamide.
CONCLUSIONS:
In conclusion, these results indicate a direct and epithelium-independent relaxant effect of Isokaempferide on smooth muscle fibers. Although this Isokaempferide relaxant action seems to be multi-mediated, it occurs via both Ca(2+) and ATP-sensitive K(+) channels, but some other possible mechanisms unrelated to K(+) channels cannot be excluded.
Citral

Catalog No: CFN70135
CAS No: 5392-40-5
Price: $30/20mg
Robinin

Catalog No: CFN98375
CAS No: 301-19-9
Price: $318/5mg
Daucosterol

Catalog No: CFN98713
CAS No: 474-58-8
Price: $80/20mg
Poricoic acid A(F)

Catalog No: CFN92838
CAS No: 137551-38-3
Price: $218/20mg
Mangiferin

Catalog No: CFN98719
CAS No: 4773-96-0
Price: $40/20mg
Neomangiferin

Catalog No: CFN98122
CAS No: 64809-67-2
Price: $80/20mg
Procyanidin B2

Catalog No: CFN99558
CAS No: 29106-49-8
Price: $138/20mg
Terpineol

Catalog No: CFN92688
CAS No: 98-55-5
Price: $30/20mg
Decursin

Catalog No: CFN98509
CAS No: 5928-25-6
Price: $138/20mg
Quercitrin

Catalog No: CFN98850
CAS No: 522-12-3
Price: $40/20mg
Tags: buy Isokaempferide | Isokaempferide supplier | purchase Isokaempferide | Isokaempferide cost | Isokaempferide manufacturer | order Isokaempferide | Isokaempferide distributor