3-Methyladenine | CAS:5142-23-4 | Manufacturer ChemFaces

3-Methyladenine

ChemFaces products have been cited in many studies from excellent and top scientific journals

Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC

How to Order

Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com

Delivery time

Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal

Citing Use of our Products

More Impact Factor than five Cite...

* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.

According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.

Size /Price /Stock	10 mM * 1 mL in DMSO / Inquiry
Other Packaging	*Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap

Featured Products

More...

Beta-Sitosterol

Catalog No: CFN99916
CAS No: 83-46-5
Price: $40/20mg

Parishin E

Catalog No: CFN93115
CAS No: 952068-57-4
Price: $298/20mg

Hederagenin

Catalog No: CFN98695
CAS No: 465-99-6
Price: $30/20mg

Taurocholic acid

Catalog No: CFN96646
CAS No: 81-24-3
Price: $ / mg

Alpha-pinene

Catalog No: CFN93285
CAS No: 80-56-8
Price: $30/20mg

Nootkatone

Catalog No: CFN98699
CAS No: 4674-50-4
Price: $40/20mg

Damulin B

Catalog No: CFN91837
CAS No: 1202868-75-4
Price: $288/5mg

N-trans-caffeoyltyramine

Catalog No: CFN95265
CAS No: 103188-48-3
Price: $318/5mg

Homoplantaginin

Catalog No: CFN90344
CAS No: 17680-84-1
Price: $138/20mg

Rebaudioside B

Catalog No: CFN90470
CAS No: 58543-17-2
Price: $138/20mg

Description:

3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation. 3-Methyladenine (3-MA) is successfully used to suppress mitophagy.

Targets:

PI3Kγ | Vps34

In vitro:

Autophagy,2010 Aug;6(6):805-7.

Finding a fitting shoe for Cinderella: searching for an autophagy inhibitor.[Pubmed: 20574157]

Vps34 is the ancestral phosphatidylinositol 3-kinase (PtdIns3K) isoform and is essential for endosomal trafficking of proteins to the vacuole/lysosome, autophagy and phagocytosis. Vps34-containing complexes associate with specific cellular compartments to produce PtdIns(3)P. Understanding the roles of Vps34 has been hampered by the lack of potent, specific inhibitors.
METHODS AND RESULTS:
To boost development of Vps34 inhibitors, we determined the crystal structures of Vps34 alone and in complexes with multitargeted PtdIns3K inhibitors. These structures provided a first glimpse into the uniquely constricted ATP-binding site of Vps34 and enabled us to model Vps34 regulation. We showed that the substrate-binding "activation" loop and the flexibly attached amphipathic C-terminal helix are crucial for catalysis on membranes. The C-terminal helix also suppresses ATP hydrolysis in the absence of membranes.
CONCLUSIONS:
We propose that membrane binding shifts the C-terminal helix to orient the enzyme for catalysis, and the Vps15 regulatory subunit, which binds to this and the preceding helix, may facilitate this process. This C-terminal region may also represent a target for specific, non-ATP-competitive PtdIns3K inhibitors.

3-Methyladenine Description

Source:
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage:	Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C). Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
After receiving:	The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.

Kinase Assay:

J Biol Chem,2010 Apr 2;285(14):10850-61.

Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase.[Pubmed: 20123989]

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.

Cell Research:

PLoS One,2012;7(4):e35665.

Inhibitors of phosphatidylinositol 3'-kinases promote mitotic cell death in HeLa cells.[Pubmed: 22545128]

Cell lines:HeLa cell line
Concentrations: 1-10 mM
Incubation Time: 24, 48 or 72 hours
Method:
Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.

Animal Research:

Neuroscience,2012 Jun 28;213:144-53.

Autophagy activation is associated with neuroprotection against apoptosis via a mitochondrial pathway in a rat model of subarachnoid hemorrhage.[Pubmed: 22521819]

Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
Dosages: 400 nM
Administration: Intracerebral ventricular

Product Name	3-Methyladenine
Price:
CAS No.:	5142-23-4
Catalog No.:	CFN60037
Molecular Formula:	C₆H₇N₅
Molecular Weight:	149.15 g/mol
Purity:	>=98%
Type of Compound:	Alkaloids
Physical Desc.:	Powder
Source:
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download:	COA MSDS
Similar structural:	Comparison
Guestbook:

Size /Price /Stock	10 mM * 100 uL in DMSO / Inquiry / In-stock 10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries	Alkaloids Compound Library

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	6.7047 mL	33.5233 mL	67.0466 mL	134.0932 mL	167.6165 mL
5 mM	1.3409 mL	6.7047 mL	13.4093 mL	26.8186 mL	33.5233 mL
10 mM	0.6705 mL	3.3523 mL	6.7047 mL	13.4093 mL	16.7616 mL
50 mM	0.1341 mL	0.6705 mL	1.3409 mL	2.6819 mL	3.3523 mL
100 mM	0.067 mL	0.3352 mL	0.6705 mL	1.3409 mL	1.6762 mL

CFN98738	Linarin	480-36-4	592.6	C₂₈H₃₂O₁₄
CFN89192	Arborine	6873-15-0	250.30	C₁₆H₁₄N₂O
CFN92978	2beta-Acetoxyferruginol	1206461-56-4	344.49	C₂₂H₃₂O₃
CFN90625	Isodemethylwedelolacton	350681-33-3	300.22	C₁₅H₈O₇