| Structure Identification: |
| Journal of Chinese Pharmaceutical Sciences, 2014, 23(7). | | HPLC fingerprint-oriented preparative separation of major flavonoids from safflower extract by preparative pressurized liquid chromatography[Reference: WebLink] | The aim of this study was to rapidly isolate the major effective flavanoids from the extract of safflower(Carthamus tinctorius) using ODS medium pressure liquid chromatography(MPLC) and semi-preparative HPLC, guided by a developed fingerprint.
METHODS AND RESULTS:
Twelve compounds were isolated and their structures were elucidated as kaempferol 3-O-β-D-rutinoside(1),
kaempferol 3-O-β-D-glucoside(2), rutin(3), quercetin 3-O-β-D-glucoside(4), 6-hydroxykaempferol 3,6,7-tri-O-β-D-glucoside(5), 6-hydroxykaempferol 3-O-β-D-glucoside(6-Hydroxykaempferol 3-beta-rutinoside, 6), 6-hydroxykaempferol 6,7-di-O-β-D-glucoside(7), 6-hydroxykaempferol 3-O-β-Drutinoside(8), 6-hydroxykaempferol 3,6-di-O-β-D-glucosyl 7-O-β-D-glucuronide(9), isosafflomin C(10), safflomin C(11) and hydroxysafflor yellow A(12) by spectroscopic analysis and comparing with the literature.
CONCLUSIONS:
Our results demonstrated that preparative pressurized liquid chromatography combined with HPLC fingerprint guide is an efficient tool to isolate the target compounds quickly. |
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