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Danaidal
Danaidal
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Danaidal
Price:
CAS No.: 27628-46-2
Catalog No.: CFN00252
Molecular Formula: C8H9NO
Molecular Weight: 135.16 g/mol
Purity: >=98%
Type of Compound: Alkaloids
Physical Desc.: Powder
Source: The male pheromones of Phragmatobia fuliginosa
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF
Similar structural: Comparison (Web)  (SDF)
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Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
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Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
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Biological Activity
Description: Reference standards.
Danaidal Description
Source: The male pheromones of Phragmatobia fuliginosa
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 7.3986 mL 36.9932 mL 73.9864 mL 147.9728 mL 184.966 mL
5 mM 1.4797 mL 7.3986 mL 14.7973 mL 29.5946 mL 36.9932 mL
10 mM 0.7399 mL 3.6993 mL 7.3986 mL 14.7973 mL 18.4966 mL
50 mM 0.148 mL 0.7399 mL 1.4797 mL 2.9595 mL 3.6993 mL
100 mM 0.074 mL 0.3699 mL 0.7399 mL 1.4797 mL 1.8497 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Animal Research:
J Chem Ecol. 1987 Apr;13(4):807-22.
Quantitative and qualitative variation in male pheromones ofPhragmatobia fuliginosa andPyrrharctia isabella (Lepidoptera: Arctiidae).[Pubmed: 24302048]
The dihydropyrrolizine pheromones, hydroxyDanaidal and Danaidal, were identified from the scent organs of malePhragmatobia fuliginosa (L.) andPyrrharctia isabella (J.E. Smith).
METHODS AND RESULTS:
Qualitative and quantitative GLC analyses were conducted on ca. 80 field-collected males of each species. The total pheromone titer was distributed bimodally in each species with most males having either a small amount (< 10 ng) of pheromone or a large amount (1-10 μg inPyrrharctia and 0.3-3 μg inPhragmatobia).Pyrrharctia males in the 1- to 10-μg range had a predominance of hydroxyDanaidal, with little if any Danaidal. MostPhragmatobia males in the 0.3- to 3-μg range had Danaidal with little if any hydroxyDanaidal. These compounds elicited a courtship response in sexually receptive females of both species. A bioassay based on this response was used to measure the thresholds of female response to these compounds.
CONCLUSIONS:
Pyrrharctia females were more sensitive to (R)-(-)-hydroxyDanaidal than to Danaidal.Phragmatobia females were more sensitive to Danaidal then to (R)-(-)-hydroxyDanaidal.
J Chem Ecol. 1989 Mar;15(3):1077-93.
Quantitative and qualitative effects of larval diet on male scent secretions ofEstigmene acrea, Phragmatobia foliginosa, andPyrrharctia isabella (Lepidoptera: Arctiidae).[Pubmed: 24271908]

METHODS AND RESULTS:
In feeding experiments with insects reared in the laboratory, the presence of the dihydropyrrolizines hydroxyDanaidal and Danaidal in the male scent organs (coremata) of the arctiids,Estigmene acrea (Drury),Phragmatobia fuliginosa (L.), andPyrrharctia isabella (J.E. Smith), was shown to depend on the presence of a source of pyrrolizidine alkaloids (PAs) in the larval diet.Phragmatobia males given an artificial diet supplemented with the powdered roots of the PA-containing plantSymphytum officinale L. (comfrey) produced more hydroxyDanaidal than Danaidal, whereas males given an artificial diet supplemented with dried whole plants of another PA-containing species,Senecio vulgaris L., produced more Danaidal than hydroxyDanaidal.Pyrrharctia males produced hydroxyDanaidal with little if any Danaidal, whether the source of PAs was comfrey orS. vulgaris. A behavioral bioassay showed that the coremata of PA-deniedPyrrharctia male progeny of PA-denied parents were pheromonally inactive, whereas those of PA-denied male progeny of PA-supplied parents (male and/or female) were often active.
CONCLUSIONS:
This indicates that a small amount of pheromone is made from PAs transferred from the female to her eggs and that males effect copulatory transfers of PAs that are, in turn, passed to the eggs by the mated female. Field observations ofPhragmatobia andPyrrharctia larvae feeding on sources of PAs were reported. The PA monocrotaline was shown to be a feeding stimulant forPyrrharctia larvae.
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