Structure Identification: |
Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(15):1973-7. | Hasubanan type alkaloids in Stephania hernandifolia.[Pubmed: 20931849] |
To study the hasubanan type alkaloids in Stephania hernandifolia.
METHODS AND RESULTS:
The dried herbs of S. hernandifolia. were extracted with 95% ethanol. After removal of the solvent, the residue was first partitioned between acid water and petroleum ether, then the aqueous layer was basified and extracted with chloroform to obtain crude alkaloids. Column chromatograghic methods with on silica gel, Rp-18, MCI CHP 20P, Sephadex LH-20 were applied for the isolation and purification of the crude alkaloid fraction. The structures were elucidated by their physicochemical properties and spectral data.
Nine hasubanan type alkaloids were obtained and identified as aknadinine(1), longanone(2), stephasunoline (3), N-methylstephuline(4), Epistephamiersine(5), prostephabyssine(6), aknadilactam(7), dihydroEpistephamiersine(8), hasubanonine(9).
CONCLUSIONS:
Compounds 2-8 were isolated from this plant for the first time. |
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