| In vitro: |
| Cancer Lett. 2007 Aug 18;253(2):224-35. | | Leachianone A as a potential anti-cancer drug by induction of apoptosis in human hepatoma HepG2 cells.[Pubmed: 17379399] | The Chinese herbal medicine Radix Sophorae is widely applied as an anti-carcinogenic/ anti-metastatic agent against liver cancer.
METHODS AND RESULTS:
In this study, Leachianone A, isolated from Radix Sophorae, possessed a profound cytotoxic activity against human hepatoma cell line HepG2 in vitro, with an IC(50) value of 3.4microg/ml post-48-h treatment. Its action mechanism via induction of apoptosis involved both extrinsic and intrinsic pathways. Its anti-tumor effect was further demonstrated in vivo by 17-54% reduction of tumor size in HepG2-bearing nude mice, in which no toxicity to the heart and liver tissues was observed.
CONCLUSIONS:
In conclusion, this is the first report describing the isolation of Leachianone A from Radix Sophorae and the molecular mechanism of its anti-proliferative effect on HepG2 cells. | | Journal of Huazhong Normal University, 2014, 48(4):520-4. | | Lavandulyl flavonoids with sodium-dependent glucose cotransporter 2 inhibitory activity from Sophora flavescens.[Reference: WebLink] | To discover new bioactive Sodium-dependent glucose cotransporter 2(SGLT2)inhibitors from the traditional Chinese medicine"Ku Shen"(roots of Sophora flavescens),the bioassay-guided purification of an active ethyl acetate fraction was performed.
METHODS AND RESULTS:
Sixteen lavandulyl flavonoids were isolated and their structures were elucidated as kushenol H(1),kushenol K(2),kurarinol(3),kushenol Y(4),kushenol P(5),norkurarinone(6),kushenol I(7),kushenol N(8),(-)-kurarinone(9),kushenol X(10),neokurarinol(11),kushenol C(12),sophoraflavanone G(13),Leachianone A(14),kuraridine(15)and kushenol A(16).All isolated compounds exhibited inhibitory activity against SGLT2.Among them,the two main constituents of the active EtOAc fraction,(-)-kurarinone(9)and sophoraflavanone G(13)showed the most potential inhibitory activity against SGLT2with the IC50values of 2.24μmol/L and 1.45μmol/L,respectively. |
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Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)
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