| In vitro: |
| Zhongguo Zhong Yao Za Zhi. 2013 Dec;38(23):4104-8. | | Cytotoxic alkaloids from stems of Nelumbo nucifera.[Pubmed: 24791498] | Chemical investigation was carried out to study the alkaloids from stems of Nelumbo nucifera and their cytotoxic activities.
METHODS AND RESULTS:
The constituents were separated by column chromatography, and their structures were elucidated by spectroscopic data analyses. The isolated compounds were evaluated for their cytotoxic activities by MTr method. Fifteen compounds were isolated from the total alkaloids extract and identified as asimilobine (1), isococlaurine (2), N-acetylNorarmepavine (3), crykonisine (4), velucryptine (5), pycnarrhine (6), liriodenine (7), nuciferine (8), nornuciferine (9), armepavine (10), N-methylasimilobine (11), coclaurine (12), N-Norarmepavine (13), N-methylcoclaurine (14) and lysicamine (15). Compounds 1-7 and 12-15 were isolated from stems of this plant for the first time, and compounds 2-6 were firstly isolated from the family Nelumbonaceae.
CONCLUSIONS:
Compounds 7-10, 13 and 14 showed significant cytotoxic activities against HL-60 carcinoma cell line with inhibitory ratios of 51.36%, 59.09%, 52.51%, 53.93%, 51.43%, and 64.31% at concentration of 1 x 10(-5) mol x L(-1), respectively. | | Planta Med. 2006 Oct;72(13):1238-41. | | Effect of isoquinoline alkaloids of different structural types on antiplatelet aggregation in vitro.[Pubmed: 16981134 ] | | Forty-one isoquinoline alkaloids were tested for antiplatelet aggregation effects. Among them, (-)-discretamine (6), protopine (7), ochotensimine (18), O-methylarmepavinemethine (23), lindoldhamine (25), isotetrandrine (26), thalicarpine (27), papaverine (28), and D-(+)- N-Norarmepavine (32) exhibited significant inhibitory activity towards adenosine 5'-diphosphate (ADP)-, arachidonic acid (AA)-, collagen-, and/or platelet-activating factor (PAF)-induced platelet aggregation. The results are discussed on the basis of structure-activity relationships. | | Comp Biochem Physiol Pharmacol Toxicol Endocrinol. 1994 Mar;107(3):367-71. | | Trypanocidal effect of boldine and related alkaloids upon several strains of Trypanosoma cruzi.[Pubmed: 8061943] |
METHODS AND RESULTS:
The alkaloids boldine, glaucine, predicentrine, apomorphine, coclaurine, Norarmepavine and codeine were tested against the epimastigotes of the Tulahuén and LQ strains and the DM 28c clone of Trypanosoma cruzi. The micromolar concentration to inhibit 50% of the culture growth (Tulahuén strain) for apomorphine, glaucine, predicentrine, boldine, Norarmepavine, coclaurine and codeine were 29, 90, 85, 110, 310, 580 and > 1000 respectively. Similar values were obtained with the LQ strain and the DM 28c clone.
CONCLUSIONS:
The most active compounds in inhibiting culture growth also inhibited cell respiration, suggesting that these drugs may act by blocking mitochondrial electron transport. The trypanocidal effects of these alkaloids appear to be correlated with their antioxidative activities. |
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