| Kinase Assay: |
| Food Science & Technology Research, 2015, 21(1):111-5. | | Inhibitory Effect of Oligomeric Polyphenols from Peanut-skin on Sugar Digestion Enzymes and Glucose Transport[Reference: WebLink] |
METHODS AND RESULTS:
Inhibitory effects of oligomeric polyphenols extracted from peanut skin on α-amylase, maltase, sucrase and glucose transport were investigated. Epicatechin-(4β→6)-epicatechin-(2β→O→7, 4β→8)-catechin (EEC) as a procyanidin trimer inhibited α-amylase activity in a dose-dependent manner, while (+)-catechin and Procyanidin A1 as a procyanidin dimer did not show any inhibitory activity up to 5 mg/mL. In the case of sucrase, EEC showed inhibitory activity stronger than (+)-catechin and Procyanidin A1. Inhibitory effects of peanut skin on glucose transport in the small intestine were also investigated. The level of glucose transport in Caco-2 cells was significantly decreased by EEC compared to (+)-catechin.
CONCLUSIONS:
These results suggest that the inhibitory effects of procyanidins on α-amylase and α-glucosidase, and the suppression of intestinal glucose transport may change with the degree of polymerization. |
|
| Structure Identification: |
| Arch Pharm Res. 2014 Nov;37(11):1403-10. | | Procyanidins from the stem wood of Machilus japonica and their inhibitory effect on LDL oxidation.[Pubmed: 24297667 ] | The stem wood of Machilus japonica Siebold & Zucc were extracted with 80 % aqueous MeOH, and the concentrated extract was successively partitioned with ethyl acetate (EtOAc), normal butanol, and water.
METHODS AND RESULTS:
From the EtOAc fraction, five procyanidins, Procyanidin A1 (1), procyanidin A2 (2), procyanidin B7 (3), cinnamtannin B1 (4), and aesculitannin B (5), were isolated. Their chemical structures were identified through spectroscopic data analyses including NMR, MS, and IR. This is the first time any of these compounds have been isolated from this plant.
CONCLUSIONS:
The compounds were evaluated for inhibition activity on LDL oxidation.
All of these compounds and the positive control, BHT, showed a very high inhibition effect with IC50 values of 0.94, 2.1, 1.8, 1.1, 1.0, and 1.9 μM, respectively. |
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