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8-Prenylkaempferol
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name 8-Prenylkaempferol
Price:
CAS No.: 28610-31-3
Catalog No.: CFN92346
Molecular Formula: C20H18O6
Molecular Weight: 354.4 g/mol
Purity: >=98%
Type of Compound: Flavonoids
Physical Desc.: Yellow powder
Source: The roots of Sophora flavescens
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
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Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
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Biological Activity
Description: 8-Prenylkaempferol is an effective agent for attenuating pro-inflammatory NO induction, it may be an anti-inflammatory agent for suppressing influenza A virus-induced RANTES production acts by blocking PI3K-mediated transcriptional activation of NF-κB and IRF-3 and in part by interfering with IκB degradation which subsequently decreases NF-κB translocation. 8-Prenylkaempferol induces differentiation/maturation of MC3T3-E1 osteoblasts by BMP-2/p38/Runx2 pathways , might be a promising agent for inducing osteogenesis.
Targets: p38MAPK | NF-kB | PI3K | NO | NOS | AP-1 | JNK | MEK | ERK | IkB | IKK
In vitro:
Life Sci. 2011 Feb 14;88(7-8):335-42.
8-Prenylkaempferol accelerates osteoblast maturation through bone morphogenetic protein-2/p38 pathway to activate Runx2 transcription.[Pubmed: 21163272]
In this study, we investigated the effect of 8-Prenylkaempferol (8-PK), a prenyl-flavonoid isolated from Sophora flavescens, on osteoblast differentiation and maturation.
METHODS AND RESULTS:
MC3T3-E1 cells were exposed to 8-Prenylkaempferol and the cytotoxicity was assayed. Osteoblast differentiation and maturation were evaluated by analyzing alkaline phosphatase (ALP) activity and cell mineralization, respectively. RT-PCR and Western blot were executed to determine the effects of 8-Prenylkaempferol on osteoblast differentiation-related gene expression and signaling pathway. 8-Prenylkaempferol significantly promoted ALP activity, up-regulated mRNA expressions of osteocalcin, osteopontin, and type I collagen, and induced bone nodules formation. Induction of differentiation by 8-Prenylkaempferol was associated with increased bone morphogenetic protein (BMP)-2 expression, and sequentially up-regulated the phosphorylations of Smad1/5/8 and p38, and increased the nuclear translocation of runt-related transcription factor 2 (Runx2). Addition of BMP-2 antagonist noggin blocked 8-Prenylkaempferol and recombinant mouse BMP-2-induced ALP activity, reconfirming that BMP-2 production is required in 8-Prenylkaempferol-mediated osteoblast differentiation. Noggin also abrogated 8-Prenylkaempferol evoked phosphorylations of Smad1/5/8 and p38, suggesting that BMP-2 signaling is required for p38 activation in 8-Prenylkaempferol-treated cells. Application of p38 inhibitor SB203580 repressed not only 8-Prenylkaempferol-mediated activation of ALP, but also the nuclear translocation of Runx2 and bone nodules formation.
CONCLUSIONS:
The present results suggested that BMP-2/p38/Runx2 pathways were involved in 8-Prenylkaempferol-induced differentiation/maturation of MC3T3-E1 osteoblasts and firstly demonstrated that 8-Prenylkaempferol might be a promising agent for inducing osteogenesis.
Evid Based Complement Alternat Med. 2011;2011:920828.
8-Prenylkaempferol Suppresses Influenza A Virus-Induced RANTES Production in A549 Cells via Blocking PI3K-Mediated Transcriptional Activation of NF-κB and IRF3.[Pubmed: 19592477]
8-Prenylkaempferol (8-PK) is a prenylflavonoid isolated from Sophora flavescens, a Chinese herb with antiviral and anti-inflammatory properties.8-Prenylkaempferol could significantly inhibit not only RANTES production but also NF-κB and IRF-3 nuclear translocation.
METHODS AND RESULTS:
We had proved that both NF-κB and IRF-3 participated in H1N1-induced RANTES production since NF-κB inhibitor pyrrolidinedithio carbamate (PDTC) and IRF-3 siRNA attenuated significantly RANTES accumulation. H1N1 inoculation also increased PI3K activity as well as Akt phosphorylation and such responsiveness were attenuated by 8-Prenylkaempferol. Furthermore, 8-Prenylkaempferol but not wortmannin, prevented effectively H1N1-evoked IκB degradation.
CONCLUSIONS:
In conclusion, 8-Prenylkaempferol might be an anti-inflammatory agent for suppressing influenza A virus-induced RANTES production acts by blocking PI3K-mediated transcriptional activation of NF-κB and IRF-3 and in part by interfering with IκB degradation which subsequently decreases NF-κB translocation.
8-Prenylkaempferol Description
Source: The roots of Sophora flavescens
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

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After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.8217 mL 14.1084 mL 28.2167 mL 56.4334 mL 70.5418 mL
5 mM 0.5643 mL 2.8217 mL 5.6433 mL 11.2867 mL 14.1084 mL
10 mM 0.2822 mL 1.4108 mL 2.8217 mL 5.6433 mL 7.0542 mL
50 mM 0.0564 mL 0.2822 mL 0.5643 mL 1.1287 mL 1.4108 mL
100 mM 0.0282 mL 0.1411 mL 0.2822 mL 0.5643 mL 0.7054 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Kinase Assay:
Eur J Pharmacol. 2008 Aug 20;590(1-3):430-6.
8-Prenylkaempferol suppresses inducible nitric oxide synthase expression through interfering with JNK-mediated AP-1 pathway in murine macrophages.[Pubmed: 18579129]
8-Prenylkaempferol is a prenylflavonoid isolated from the roots of Sophora flavescens, a Chinese herb with anti-inflammatory properties. However whether 8-Prenylkaempferol itself displayed an anti-inflammatory activity remained unclear.
METHODS AND RESULTS:
In this study, we evaluated the effect of 8-Prenylkaempferol on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 macrophages. 8-Prenylkaempferol inhibited significantly LPS-induced NO production through suppressing inducible NO synthase (iNOS) expression at both protein and mRNA levels but failed to affect sodium nitroprusside-triggered NO production, iNOS enzyme activity, and cell viability. Further investigation of the mechanisms revealed that 8-Prenylkaempferol inhibited LPS-induced c-Jun phosphorylation (a major component of activator protein-1, AP-1), but did not attenuate IkB-alpha degradation nor NF-kappaB nuclear translocation. Cellular signaling analysis using mitogen-activating protein kinase (MAPK) inhibitors including 2'-amino-3'-methoxyflavone (PD98059, MEK1/2 inhibitor), 4-[5-(4-fluorophenyl)-2-[4-(methylsulfonyl)phenyl]-1H-imidazol-4-yl]pyridine (SB203580, p38 kinase inhibitor) and anthra[1-9-cd]pyrazol-6(2H)-one (SP600125, c-Jun N-terminal kinase inhibitor) demonstrated that extracellular signal-regulated kinase1/2 (ERK1/2), p38 and JNK all participated in LPS-stimulated iNOS expression and NO production, but 8-Prenylkaempferol interfered selectively with JNK phosphorylation. On the other hand, LPS-induced c-Jun phosphorylation was attenuated in the presence of SP600125.
CONCLUSIONS:
We suggested that interfering with JNK-mediated c-Jun phosphorylation and thus blocking AP-1 activation might contribute to the suppression effects of 8-Prenylkaempferol on iNOS. These findings provided the first molecular basis that 8-Prenylkaempferol is an effective agent for attenuating pro-inflammatory NO induction.
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