| In vitro: |
| Planta Med. 2001 Jul;67(5):437-42. | | Further phenols and polyacetylenes from the rhizomes of Atractylodes lancea and their anti-inflammatory activity.[Pubmed: 11488458 ] |
METHODS AND RESULTS:
From the rhizomes of Atractylodes lancea, 2-[(2'E)-3',7'-dimethyl-2',6'-octadienyl]-4-methoxy-6-methylphenol (1) was isolated as a new natural product. The compound showed strong inhibitory effects on 5-lipoxygenase (5-LOX) and cyclooxygenase-1 (COX-1), but exhibited only weak antioxidative activities [IC50 = 0.1 microM (5-LOX), 2 microM (COX-1), 9 microM (PMN/FMLP), 28 microM (PMNIOZ)]. Moreover, five new acetylenes were isolated and elucidated as (3Z,5E,11E)-tridecatriene-7,9-diynyl-1-O-(E)-ferulate (2), erythro-(1,3Z,11E)-tridecatriene-7,9-diyne-5,6-diyl diacetate (3), (1Z)-atractylodin (4), (1Z)-atractylodinol (5), (1Z)-Acetylatractylodinol (6) plus the known (4E,6E,12E)-tetradecatriene-8,10-diyne-1,3-diyl diacetate (7).
CONCLUSIONS:
Among the acetylenes, only 2 showed strong inhibition of 5-LOX and COX-1 activity (IC50 (5-LOX) = 3 microM, IC50 (COX-1) = 1 microM).
In addition, the fatty acids linoleic acid, oleic acid and palmitic acid with previously established 5-LOX-/COX-1 inhibitory actions were identified as major constituents of the n-hexane extract and thus seem to contribute to the plant's in vitro activity. | | Pharmaceutical and pharmacological letters,1998,8(2):69-71. | | Antioxidant activity of constituents from Atractylodes lancea.[Reference: WebLink] | Four polyacetylenes atractylodin, Acetylatractylodinol, 1-(2-furyl)-(7E)-nonene-3,5-diyne-1,2-diacetate, erythro-(1,5E, 11E)-tridecatriene-7,9-diyne-3,4-diacetate,as well as atractylochromene (I) and 2-[(2E)-3,7-dimethyl-2,6-octadienyl]-6-methyl-2,5-cyclohexadiene-1,4-dione (II) isolated from the rhizomes of Atractylodes lancea were evaluated for their antioxidant activity ex vivo.
METHODS AND RESULTS:
When measuring the inhibition of luminal enhanced chemiluminescence in stimulated human neutrophils, only the chromene I and the quinone II showed strong antioxidant activity. After stimulation with FMLP or opsonized zymosan (OZ) both compds. exhibited comparable inhibitory effects (IC50 I = 1.3 micro M (FMLP), 5.6 micro M (OZ); IC50 II = 1.1 micro M (FMLP), 5.4 micro M (OZ)). In contrast, I was considerably more active in a cell free in vitro assay with H2O2 and horseradish peroxidase (IC50 = 4.9 micro M I, 11 micro M II). |
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