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Aloin B
Aloin B
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Aloin B
Price: $138 / 20mg
CAS No.: 28371-16-6
Catalog No.: CFN90434
Molecular Formula: C21H22O9
Molecular Weight: 418.39 g/mol
Purity: >=98%
Type of Compound: Anthraquinones
Physical Desc.: Yellow powder
Source: The herbs of Aloe vera L.
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Download: COA    MSDS    SDF    Manual
Similar structural: Comparison (Web)  (SDF)
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According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $29.6 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
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Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
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Biological Activity
Description: Dietary supplementation of aloe components (aloin, aloesin and aloe-gel) can ameliorate intestinal inflammatory responses in a 3% dextran sulfate sodium (DSS)-induced ulcerative colitis rat model, in particular, aloesin is the most potent inhibitor. The extract of A. vera and its active ingredient aloin cause melanin aggregation leading to skin lightening via alpha adrenergic receptor stimulation.
In vitro:
J AOAC Int. 2014 Sep-Oct;97(5):1323-8.
Determination of Aloin A and Aloin B in Aloe vera Raw Materials and Finished Products by High-Performance Liquid Chromatography: Single-Laboratory Validation.[Pubmed: 25902982]
A single-laboratory validation (SLV) was conducted on an HPLC method for the detection and quantification of aloin A and Aloin B in Aloe vera raw materials and finished products.
METHODS AND RESULTS:
An extraction procedure using sonication with an acidified solvent was used for solid test materials while liquid test materials only required dilution, if necessary, prior to filtration and analysis. Separation was achieved using a fused core C18 column in 18 min under isocratic elution conditions allowing for a single analyte (aloin A) calibration curve to quantify both aloins. Adequate chromatographic resolution (Rs ≥1) was achieved for aloin A and Aloin B. The calibration curves for aloin A exhibited coefficients of determination (r(2)) of ≥99.9% over the linear range of 0.3-50 μg/mL.
CONCLUSIONS:
The LOD values were 0.092 and 0.087 μg/mL, and LOQ 0.23 and 0.21 μg/mL for aloin A and Aloin B, respectively.
Aloin B Description
Source: The herbs of Aloe vera L.
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
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PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
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IF=14.548(2019)

PMID: 29149595

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doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

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Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.3901 mL 11.9506 mL 23.9011 mL 47.8023 mL 59.7529 mL
5 mM 0.478 mL 2.3901 mL 4.7802 mL 9.5605 mL 11.9506 mL
10 mM 0.239 mL 1.1951 mL 2.3901 mL 4.7802 mL 5.9753 mL
50 mM 0.0478 mL 0.239 mL 0.478 mL 0.956 mL 1.1951 mL
100 mM 0.0239 mL 0.1195 mL 0.239 mL 0.478 mL 0.5975 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Structure Identification:
Advanced Materials Research, Vols. 634-638, pp. 1241-1246, Jan. 2013
Isolation and Purification of Aloin a and Aloin B by High-Speed Counter-Current Chromatography[Reference: WebLink]

METHODS AND RESULTS:
High-speed counter-current chromatography(HSCCC) was successfully used for isolation and purification of Aloin A and Aloin B from the crude methanol extract of Aloe with a two-phase solvent system composed of chloroform–methanol–n-butylalcohol-water at an optimized volume ratio of 4:3:1:2 (v/v/v/v). The lower phase was used as the mobile phase in the head to tail elution mode. The preparative HSCCC separation was performed on 180 mg of the crude extract yielding pure Aloin A(18mg) and Aloin B(16mg) at purities of 95.2% and 96.8%, respectively, as determined by high performance liquid chromatography (HPLC).
CONCLUSIONS:
HSCCC is a powerful technique for isolation and separation of chemical composition from aloe.
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