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Calyxin H
Calyxin H
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Calyxin H
Price:
CAS No.: 202596-22-3
Catalog No.: CFN98024
Molecular Formula: C35H34O7
Molecular Weight: 566.7 g/mol
Purity: >=98%
Type of Compound: Chalcones
Physical Desc.: Yellow powder
Source: The seeds of Alpinia katsumadai
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF    Manual
Similar structural: Comparison (Web)  (SDF)
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Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
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Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Calyxin H at 1uM can increase the expression of heat shock factor 1 (HSF1), it has a possible application as a heat shock protein (HSP) inducer.
Targets: HSP (e.g. HSP90)
In vitro:
J Nat Prod. 2011 Oct 28;74(10):2109-15.
Diarylheptanoids from the seeds of Alpinia katsumadai as heat shock factor 1 inducers.[Pubmed: 21942765]

METHODS AND RESULTS:
Seven new diarylheptanoids, (-)-(R)-4″-hydroxyyashabushiketol (1), (3S,5S)-alpinikatin (2), katsumain C (3), 7-epi-katsumain C (4), ent-alpinnanin B (5), ent-alpinnanin A (6), and ent-Calyxin H (8), were isolated from the EtOAc extract of the seeds of Alpinia katsumadai together with three known compounds, alpinnanin B (7), epiCalyxin H (9), and Calyxin H (10). Each isomer mixture of 3 and 4, 5-7, and 8-10 was separated successfully by preparative HPLC using a chiral column. The three isomer mixtures (3 and 4, 5-7, 8-10) at 1 μM increased expression of heat shock factor 1 (HSF1) with fold increases of 1.438, 1.190, and 1.316, respectively, which was accompanied with increased expression of heat shock protein (HSP) 27 (1.403-, 1.250-, and 1.270-fold, respectively) and HSP70 (1.373-, 1.313-, and 1.229-fold, respectively) without cellular cytotoxicity, suggesting a possible application of these compounds as HSP inducers.
CONCLUSIONS:
Celastrol was used as a positive control of HSP induction, producing fold increases of 1.066 (HSF1), 1.216 (HSP27), and 1.371 (HSP70) at 1 μM. Compounds 1 and 2 did not affect the induction of HSF1 protein.
Calyxin H Description
Source: The seeds of Alpinia katsumadai
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.7646 mL 8.823 mL 17.646 mL 35.292 mL 44.1151 mL
5 mM 0.3529 mL 1.7646 mL 3.5292 mL 7.0584 mL 8.823 mL
10 mM 0.1765 mL 0.8823 mL 1.7646 mL 3.5292 mL 4.4115 mL
50 mM 0.0353 mL 0.1765 mL 0.3529 mL 0.7058 mL 0.8823 mL
100 mM 0.0176 mL 0.0882 mL 0.1765 mL 0.3529 mL 0.4412 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Structure Identification:
J Nat Prod. 1998 Feb 27;61(2):212-6.
Calyxin H, Epicalyxin H, and Blepharocalyxins A and B, Novel Diarylheptanoids from the Seeds of Alpinia blepharocalyx[Pubmed: 9548849]

METHODS AND RESULTS:
Four unprecedented diarylheptanoids-Calyxin H (1) and epiCalyxin H (2), possessing a diarylheptanoid unit and a chalcone moiety, and blepharocalyxins A (3) and B (4), possessing two diarylheptanoid units and a chalcone moiety-were isolated from the seeds of Alpiniablepharocalyx.
CONCLUSIONS:
The structures of 1-4, including absolute stereochemistry, were elucidated by spectroscopic means and after a consideration of their biogenesis.
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