Science | Nature | Cell | View More
Natural Products
Irilone
Irilone
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Irilone
Price: $333 / 5mg
CAS No.: 41653-81-0
Catalog No.: CFN93694
Molecular Formula: C16H10O6
Molecular Weight: 298.25 g/mol
Purity: >=98%
Type of Compound: Flavonoids
Physical Desc.: Powder
Source: The herbs of Red Clover ( Trifolium pratense)
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS
Similar structural: Comparison (Web)  (SDF)
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $233.1 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • University of Bordeaux (France)
  • Kyushu University (Japan)
  • Regional Crop Research Institute (Korea)
  • Universidad Miguel Hernández (Spain)
  • University of Parma (Italy)
  • University of Liège (Belgium)
  • Srinakharinwirot University (Thailand)
  • Celltrion Chemical Research Ins... (Korea)
  • Warszawski Uniwersytet Medyczny (Poland)
  • Julius Kühn-Institut (Germany)
  • University of Bonn (Germany)
  • More...
Package
Featured Products
Pueroside A

Catalog No: CFN95177
CAS No: 100692-52-2
Price: $318/5mg
20-Hydroxyecdysone

Catalog No: CFN98873
CAS No: 5289-74-7
Price: $40/20mg
Baicalin

Catalog No: CFN99111
CAS No: 21967-41-9
Price: $40/20mg
Methylophiopogonanone B

Catalog No: CFN98596
CAS No: 74805-91-7
Price: $268/20mg
Senecionine

Catalog No: CFN00417
CAS No: 130-01-8
Price: $ /
Clematichinenoside AR

Catalog No: CFN93289
CAS No: 761425-93-8
Price: $238/10mg
3',4',5'-Trimethoxyflavone

Catalog No: CFN70439
CAS No: 67858-30-4
Price: $118/10mg
4-Hydroxybenzyl alcohol

Catalog No: CFN97071
CAS No: 623-05-2
Price: $30/20mg
Isomangiferin

Catalog No: CFN90385
CAS No: 24699-16-9
Price: $238/20mg
Ginsenoside Rb3

Catalog No: CFN99966
CAS No: 68406-26-8
Price: $70/20mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Irilone has immunomodulatory, and α-amylase inhibitory activities, it exhibits the selective inhibition toward CYP3 A4 rather than other major human CYPs. Irilone exhibited prominent antioxidant activities with the IC50 value of 10.46μM. Irilone potentiated the effect of progesterone in both endometrial and ovarian cancer cell lines, it protected dopaminergic neurons against LPS-induced injury through inhibition of microglia activation and proinflammatory factors generation.
Targets: CYP3 A4 | IL Recepter | IFN-γ
In vitro:
J Nat Prod. 2018 Sep 28;81(9):1962-1967.
Irilone from Red Clover ( Trifolium pratense) Potentiates Progesterone Signaling.[Pubmed: 30199256 ]
The use of botanical dietary supplements is becoming increasingly popular for the alleviation of hormonal-based conditions such as hot flashes, premenstrual syndrome, and fertility. Estrogen and progesterone receptors (ER and PR) play an essential role in these processes. However, despite the fact that many therapies used to alleviate gynecological conditions act through PR-mediated mechanisms, few studies have investigated or identified any herbal natural product components that act on this receptor.
METHODS AND RESULTS:
In the current study, we used a progesterone response element (PRE)-luciferase (Luc) reporter assay to identify four phytoprogestins present in a standardized red clover ( Trifolium pratense) extract. We found that the component Irilone (1) potentiated the effect of progesterone in both endometrial and ovarian cancer cell lines. In these cancers, progesterone action is generally associated with positive outcomes; thus the potentiating effect of 1 may provide entirely new strategies for enhancing progesterone signaling as a means of mitigating conditions such as fibroids and endometriosis. Formononetin (3) and biochanin A (4) exhibited mixed agonist activity, while prunetin (2) acted only as an antagonist.
CONCLUSIONS:
Collectively, these results suggest that the effects of red clover extract repeatedly observed in cultured cells and the inverse correlation between risk of various cancers and flavonoid intake may be due, in part, to altered progesterone signaling.
Bioorg Chem. 2017 Feb;70:192-198.
8-Hydroxyirilone 5-methyl ether and 8-hydroxyirilone, new antioxidant and α-amylase inhibitors isoflavonoids from Iris germanica rhizomes.[Pubmed: 28069265 ]
Iris species are well recognized as wealthy sources of isoflavonoids.
METHODS AND RESULTS:
In the present study, phytochemical investigation of the rhizomes of Iris germanica (Iridaceae) procure the isolation of two new isoflavonoids namely, 8-hydroxyIrilone 5-methyl ether (2) and 8-hydroxyIrilone (3), along with eight known isoflavonoids: Irilone 4'-methyl ether (1), Irilone (4), irisolidone (5), irigenin S (6), irigenin (7), Irilone 4'-O-β-d-glucopyranoside (8), iridin S (9), and iridin (10). The isolated flavonoids were structurally characterized with the assist of comprehensive spectroscopic analyses (UV, IR, 1D and 2D NMR, and HRMS) and comparing with the published data. They were estimated for their antioxidant and antidaibetic capacities using DPPH and α-amylase inhibition assays, respectively.
CONCLUSIONS:
Compounds 2, 3, and 4 exhibited prominent antioxidant activities with IC50 values of 12.92, 9.23, and 10.46μM, respectively compared to propyl gallate (IC50 7.11μM). Moreover, 2-5 possessed highest α-amylase inhibitory activity with % inhibition 66.1, 78.3, 67.3, and 70.1, respectively in comparison to acarbose (reference α-amylase inhibitor). Additionally, their structure-activity relationship has been discussed.
Neurosci Res. 2008 Oct;62(2):123-30.
Protective effect of isoflavones from Trifolium pratense on dopaminergic neurons.[Pubmed: 18675857 ]
In the present study, protective effect of five isoflavones (formononetin, daidzein, pratensein, calycosin and Irilone) from Trifolium pratense on lipopolysaccharide-induced dopaminergic neurodegeneration was studied for the first time.
METHODS AND RESULTS:
The results showed that all five isoflavones attenuated LPS-induced decrease in dopamine uptake and the number of dopaminergic neurons in a dose-dependent manner in rat mesencephalic neuron-glia cultures. Moreover, they also significantly inhibited LPS-induced activation of microglia and production of tumor necrosis factor-alpha, nitric oxide and superoxide in mesencephalic neuron-glia cultures and microglia-enriched cultures. In addition, the rank order of protective potency of five isoflavones was: pratensein>daidzein>calycosin>formononetin>Irilone.
CONCLUSIONS:
This study suggested that all five isoflavones protected dopaminergic neurons against LPS-induced injury through inhibition of microglia activation and proinflammatory factors generation.
In vivo:
Phytother Res. 2009 Mar;23(3):428-33
Immunomodulatory activity of isoflavones isolated from Iris germanica (Iridaceae) on T-lymphocytes and cytokines.[Pubmed: 19003948 ]
The immunomodulatory activities of two isoflavones, 5,7-dihydroxy-6,4'-dimethoxyisoflavone (irisolidone) (1) and 5,4'-dihydroxy-6,7-methylenedioxyisoflavone (Irilone) (2) isolated from Iris germanica (Iridaceae) is reported.
METHODS AND RESULTS:
Their influence on production of T-lymphocytes (CD4+ and CD8+ cells) and T-cell cytokines, namely Th1: IL-2, IFN-gamma and Th2: IL-4 and IL-5 in a dose-dependent manner was studied by flow cytometric method in Balb/c mice. Oral administration of drugs at doses of 0.1-0.8 mg/kg per oral dose showed 1 to possess stimulatory activity on T-cells and Th1 cytokine production, while as 2 acted as an immunosuppressant for both cells and cytokines.
CONCLUSIONS:
The methylated products of 1 and 2 showed a similar trend to that of their parent compounds but their activity was drastically decreased revealing the importance of free phenolic groups for their immunomodulating activities.
Irilone Description
Source: The herbs of Red Clover ( Trifolium pratense)
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
(R)-O-isobutyroyllomatin

Catalog No: CFN95250
CAS No: 440094-38-2
Price: $413/5mg
Quercetin-3'-glucoside

Catalog No: CFN95271
CAS No: 19254-30-9
Price: $318/10mg
4-O-Coumaroylquinic acid

Catalog No: CFN95508
CAS No: 53539-37-0
Price: $318/5mg
5,7,2',4'-Tetrahydroxy-8,3'-di(gam...

Catalog No: CFN95084
CAS No: 141846-47-1
Price: $413/5mg
Phloretin 3',5'-Di-C-glucoside

Catalog No: CFN95306
CAS No: 357401-40-2
Price: $318/10mg
Resinacein D

Catalog No: CFN95591
CAS No: 2231061-47-3
Price: $413/5mg
8-Hydroxypinoresinol-4'-O-beta-D-g...

Catalog No: CFN95356
CAS No: 102582-69-4
Price: $318/5mg
Isonardosinone

Catalog No: CFN95180
CAS No: 27062-01-7
Price: $318/10mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 3.3529 mL 16.7645 mL 33.5289 mL 67.0578 mL 83.8223 mL
5 mM 0.6706 mL 3.3529 mL 6.7058 mL 13.4116 mL 16.7645 mL
10 mM 0.3353 mL 1.6764 mL 3.3529 mL 6.7058 mL 8.3822 mL
50 mM 0.0671 mL 0.3353 mL 0.6706 mL 1.3412 mL 1.6764 mL
100 mM 0.0335 mL 0.1676 mL 0.3353 mL 0.6706 mL 0.8382 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Kinase Assay:
Toxicol Lett. 2018 Sep 15;294:27-36.
Drug interaction study of flavonoids toward CYP3A4 and their quantitative structure activity relationship (QSAR) analysis for predicting potential effects.[Pubmed: 29753067 ]
The high risk of herb-drug interactions (HDIs) mediated by the herbal medicines and dietary supplements which containing abundant flavonoids had become more and more frequent in our daily life. In our study, the inhibition activities of 44 different structures of flavonoids toward human CYPs were systemically evaluated for the first time.
METHODS AND RESULTS:
According to our results, a remarkable structure-dependent inhibition behavior toward CYP3A4 was observed in vitro. Some flavonoids such as licoflavone (12) and Irilone (30) exhibited the selective inhibition toward CYP3 A4 rather than other major human CYPs. To illustrate the interaction mechanism, the inhibition kinetics of various compounds was further performed. Sophoranone (1), apigenin (10), baicalein (11), 5,4'-dihydroxy-3,6,7,8,3'-pentamethoxyflavone (15), myricetin (23) and kushenol K (38) remarkably inhibited the CYP3 A4-catalyzed bufalin 5'-hydroxylation reaction, with Ki values of 2.17 ± 0.29, 6.15 ± 0.39, 9.18 ± 3.40, 2.30 ± 0.36, 5.00 ± 2.77 and 1.35 ± 0.25 μM, respectively. Importantly, compounds 1, 11, 15, 23 and 38 could significantly inhibit the metabolism of some clinical drugs in vitro, and these drug-drug interactions (DDIs) of myricetin (23) or kushenol K (38) with clinical drug diazepam were further verified in human primary hepatocytes, respectively. Finally, a quantitative structure-activity relationship (QSAR) of flavonoids with their inhibitory effects toward CYP3 A4 was established using computational methods.
CONCLUSIONS:
Our findings illustrated the high risk of herb-drug interactions (HDIs) caused by flavonoids and revealed the vital structures requirement of natural flavonoids for the HDIs with clinical drugs eliminated by CYP3 A4. Our research provided the useful guidance to safely and rationally use herbal medicines and dietary supplements containing rich natural flavonoids components.
Pueroside A

Catalog No: CFN95177
CAS No: 100692-52-2
Price: $318/5mg
20-Hydroxyecdysone

Catalog No: CFN98873
CAS No: 5289-74-7
Price: $40/20mg
Baicalin

Catalog No: CFN99111
CAS No: 21967-41-9
Price: $40/20mg
Methylophiopogonanone B

Catalog No: CFN98596
CAS No: 74805-91-7
Price: $268/20mg
Senecionine

Catalog No: CFN00417
CAS No: 130-01-8
Price: $ /
Clematichinenoside AR

Catalog No: CFN93289
CAS No: 761425-93-8
Price: $238/10mg
3',4',5'-Trimethoxyflavone

Catalog No: CFN70439
CAS No: 67858-30-4
Price: $118/10mg
4-Hydroxybenzyl alcohol

Catalog No: CFN97071
CAS No: 623-05-2
Price: $30/20mg
Isomangiferin

Catalog No: CFN90385
CAS No: 24699-16-9
Price: $238/20mg
Ginsenoside Rb3

Catalog No: CFN99966
CAS No: 68406-26-8
Price: $70/20mg
Tags: buy Irilone | Irilone supplier | purchase Irilone | Irilone cost | Irilone manufacturer | order Irilone | Irilone distributor