Science | Nature | Cell | View More
Natural Products
Jolkinolide A
Jolkinolide A
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Jolkinolide A
Price:
CAS No.: 37905-07-0
Catalog No.: CFN92871
Molecular Formula: C20H26O3
Molecular Weight: 314.4 g/mol
Purity: >=98%
Type of Compound: Diterpenoids
Physical Desc.: Cryst.
Source: The herbs of Stellera chamaejasme Linn.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF    Manual
Similar structural: Comparison (Web)  (SDF)
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • Medizinische Universit?t Wien (Austria)
  • University of Pretoria (South Africa)
  • Universidad Industrial de Santa... (Colombia)
  • The Vancouver Prostate Centre (... (Canada)
  • The Australian National University (Australia)
  • University of Beira Interior (Portugal)
  • University of East Anglia (United Kingdom)
  • Leibniz-Institut für Pflanzenb... (Germany)
  • Aveiro University (Portugal)
  • Mahidol University (Thailand)
  • University of Toronto (Canada)
  • More...
Package
Featured Products
Epiberberine

Catalog No: CFN98564
CAS No: 6873-09-2
Price: $168/20mg
Betulinic acid

Catalog No: CFN98706
CAS No: 472-15-1
Price: $40/20mg
Echinocystic acid

Catalog No: CFN98812
CAS No: 510-30-5
Price: $60/20mg
3,29-Dibenzoyl karounitriol

Catalog No: CFN90576
CAS No: 873001-54-8
Price: $218/20mg
Isoimperatorin

Catalog No: CFN99107
CAS No: 482-45-1
Price: $40/20mg
Ginsenoside Rk1

Catalog No: CFN92644
CAS No: 494753-69-4
Price: $188/20mg
Ononin

Catalog No: CFN99136
CAS No: 486-62-4
Price: $50/20mg
Curcumin

Catalog No: CFN98686
CAS No: 458-37-7
Price: $30/20mg
Retronecine

Catalog No: CFN00322
CAS No: 480-85-3
Price: $ /
Polygalacin D

Catalog No: CFN93351
CAS No: 66663-91-0
Price: $/mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Jolkinolide A has anti-tumor activity. It inhibited VEGF expression in A549 cells through the inhibition of the Akt-STAT3-mTOR signaling pathway, and directly inhibited the proliferation and migration of HUVECs.
Targets: VEGF | Akt | Akt | STAT | mTOR
In vitro:
Medical Science Monitor, 2017, 23:223-237.
Jolkinolide A and Jolkinolide B Inhibit Proliferation of A549 Cells and Activity of Human Umbilical Vein Endothelial Cells.[Pubmed: 28087861]
Jolkinolide A (JA) and Jolkinolide B (JB) are diterpenoids extracted from the roots of Euphorbia fischeriana Steud and have been shown to have anti-tumor activity. However, their effects on the ability of tumor cells to invade blood vessels and metastasize remain largely unknown. Investigations into the effects of JA and JB on the angiogenesis of tumor tissues may facilitate the identification of new natural drugs with anti-tumor growth and metastasis activities.
METHODS AND RESULTS:
We used different concentrations of JA and JB (20 μg/ml, 40 μg/ml, 60 μg/ml, 80 μg/ml, and 100 μg/ml) to stimulate A549 cells and then studied the effects on the growth and metastasis of lung cancers. In addition, we used conditional media from A549 cells (A549-CM) stimulated by either JA or JB in different concentrations to culture human umbilical vein endothelial cells (HUVECs). We found that both JA and JB significantly inhibited the Akt-STAT3-mTOR signaling pathway and reduced the expression of VEGF in A549 cells, but JB exhibited more significant inhibitory effects than JA. The JB-stimulated A549 cell conditional media had a greater inhibitory effect on the proliferation and migration of HUVECs than did the conditional media of JA-stimulated A549 cells. This effect gradually increased with increasing concentrations of either type of Jolkinolide.
CONCLUSIONS:
Our results suggest that JA and JB inhibited VEGF expression in A549 cells through the inhibition of the Akt-STAT3-mTOR signaling pathway, and directly inhibited the proliferation and migration of HUVECs. These findings are of great significance for the development of new plant-derived chemotherapy agents for the treatment of cancer.
Planta Medica, 2005, 71(4):349-354.
Cytotoxic diterpenoids from the roots of Euphorbia ebracteolata.[Pubmed: 15856412]

METHODS AND RESULTS:
Three new diterpenoids, yuexiandajisu D (1), E (2) and F were isolated from the roots of Euphorbia ebracteolata, along with eight known diterpenoids, jolkinolide B (4), Jolkinolide A, ent-11alpha-hydroxyabieta-8(14),13(15)-dien-16,12alpha-olide (6), ent-(13S)-hydroxyatis-16-ene-3,14-dione, ent-3beta,(13S)-dihydroxyatis-16-en-14-one, ent-3-oxokaurane-16alpha,17-diol, ent-16alpha,17-dihydroxyatisan-3-one and ent-atisane-3beta,16alpha,17-triol. The structures of all compounds were deduced using spectroscopic methods and confirmed for 1 and 2 by single-crystal X-ray diffraction. A biogenetic pathway for the formation of 1 and 2 is proposed briefly. Cytotoxic activities were evaluated against ANA-1, B 16 and Jurkat tumor cells.
CONCLUSIONS:
Jolkinolide B (4) displayed modest activity on ANA-1, B 16 and Jurkat tumor cells with IC50 values 4.46 x 10(-2), 4.48 x 10(-2), 6.47 x 10(-2) microM, and ent-11alpha-hydroxyabieta-8(14),13(15)-dien-16,12alpha-olide (6) showed significant activity against ANA-1 and Jurkat cells with IC50 values 7.12 x 10(-3) and 1.79 x 10(-2) microM. Compound 1 was found to be slightly active against ANA-1 cells with an IC50 value 2.88 x 10(-1)microM. Structure-activity relationships of isolated compounds are also discussed.
Jolkinolide A Description
Source: The herbs of Stellera chamaejasme Linn.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
Betmidin

Catalog No: CFN95047
CAS No: 35589-22-1
Price: $413/5mg
3'-Hydroxy-2,4,5-trimethoxydalberg...

Catalog No: CFN95410
CAS No: N/A
Price: $413/5mg
Yinxiancaoside C

Catalog No: CFN95377
CAS No: 1159908-74-3
Price: $413/5mg
3''-Galloylquercitrin

Catalog No: CFN95048
CAS No: 503446-90-0
Price: $368/5mg
Meliotocarpan D

Catalog No: CFN95401
CAS No: 83013-81-4
Price: $413/5mg
Regaloside I

Catalog No: CFN95525
CAS No: 126239-78-9
Price: $318/5mg
Andrographidine A

Catalog No: CFN95210
CAS No: 113963-37-4
Price: $318/10mg
Silybin A

Catalog No: CFN95149
CAS No: 22888-70-6
Price: $318/20mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 3.1807 mL 15.9033 mL 31.8066 mL 63.6132 mL 79.5165 mL
5 mM 0.6361 mL 3.1807 mL 6.3613 mL 12.7226 mL 15.9033 mL
10 mM 0.3181 mL 1.5903 mL 3.1807 mL 6.3613 mL 7.9517 mL
50 mM 0.0636 mL 0.3181 mL 0.6361 mL 1.2723 mL 1.5903 mL
100 mM 0.0318 mL 0.159 mL 0.3181 mL 0.6361 mL 0.7952 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Structure Identification:
Chinese Journal of Pharmaceutical Analysis (2006) 26(9) 1204-1206.
HPLC determination of jolkinolide A and jolkinolide B in Chinese herb medicine" Lang- du"[Reference: WebLink]
To establish a method for the determination of two diterpenoide lactones, Jolkinolide A and jolkinolide B in Chinese herbal medicine "Lang -du" by HPLC.
METHODS AND RESULTS:
: C18 column(4.6 mm *250 mm,5 mum) was used with acetonitrile -water(75:25 )as the mobile phase. The flow rate was 1.0 mL· min^-1 ,and the detection wavelength was 250 nm. By using the established HPLC method, nine samples of "Lang - du" were analyzed to determine the contents of Jolkinolide A and B. It was showed that the contents of Jolkinolide A and B ranged from 0. 007% to 0. 043% and 0. 012% to 0. 204% , respectively. Among the samples, the two diterpenoids were found in most of the samples of Euphorbia fischeriana and E. ebracteolata, but Stellera chamaejasme and Alocasia macrorrhiza contained neither Jolkinolide A nor jolkinolide B.
CONCLUSIONS:
A simple, effective and feasible HPLC method with good accuracy, precision and reproducibility to determine the contents of two diterpenoids in" Lang - du" was established which could be applied for the quality control of this herb.
Epiberberine

Catalog No: CFN98564
CAS No: 6873-09-2
Price: $168/20mg
Betulinic acid

Catalog No: CFN98706
CAS No: 472-15-1
Price: $40/20mg
Echinocystic acid

Catalog No: CFN98812
CAS No: 510-30-5
Price: $60/20mg
3,29-Dibenzoyl karounitriol

Catalog No: CFN90576
CAS No: 873001-54-8
Price: $218/20mg
Isoimperatorin

Catalog No: CFN99107
CAS No: 482-45-1
Price: $40/20mg
Ginsenoside Rk1

Catalog No: CFN92644
CAS No: 494753-69-4
Price: $188/20mg
Ononin

Catalog No: CFN99136
CAS No: 486-62-4
Price: $50/20mg
Curcumin

Catalog No: CFN98686
CAS No: 458-37-7
Price: $30/20mg
Retronecine

Catalog No: CFN00322
CAS No: 480-85-3
Price: $ /
Polygalacin D

Catalog No: CFN93351
CAS No: 66663-91-0
Price: $/mg
Tags: buy Jolkinolide A | Jolkinolide A supplier | purchase Jolkinolide A | Jolkinolide A cost | Jolkinolide A manufacturer | order Jolkinolide A | Jolkinolide A distributor