| In vitro: |
| Chinese Traditional & Herbal Drugs, 2013, 44(12):1552-1557. | | Chemical constituents of triterpenoid saponins from Glycyrrhiza uralensis.[Reference: WebLink] | To study the chemical constituents from the roots and rhizomes of Glycyrrhiza uralensis.
METHODS AND RESULTS:
The compounds were separated and purified by solvent and chromatographic methods. Their structures were identified by spectroscopic techniques. Fourteen triterpenoid saponins isolated from 50% ethanol extract of the roots and rhizomes of G. uralensis were identified as uralsaponin C (1), uralsaponin D (2), Licoricesaponin A3 (3), uralsaponin F (4), 22β-acetoxyl-glycyrrizin (5), 24-hydroxyl-licorice-saponin E2 (6), licorice-saponin E2 (7), licorice-saponin G2 (8), 22β-acetoxyl-glyrrhaldehyde (9), 3β-O-[β-D-glucuronopyranosyl-(1→2) - β-D-glucuronopyranosyl]-glycyrretol (10), araboglycyrrhizin (11), licorice-saponin J2 (12), glycyrrhizin (13), and glycyrrhetic acid monoglucuronide (14). Compounds 1-14 showed the cytotoxic activity against the human cancer cell lines MGC-803, SW620, and SMMC-7721 with IC50 > 100 μmol/L. The aglycones of compounds 2, 6-8, and 13 displayed the inhibition on the growth of cancer cells with IC50 at 18.3-41.6 μmol/L.
CONCLUSIONS:
Compound 14 is a new natural product, and compound 11 is isolated from the plant for the first time; Compounds 1-14 show no cytotoxic activity against the human cancer cell lines MGC-803, SW620, and SMMC-7721, and the aglycones of compounds 2, 6-8, and 13 could significantly increase the cytotoxic activity after hydrolysis. | | Arch Pharm Res. 2012 Nov;35(11):1945-52. | | Comparative study of bioactive constituents in crude and processed Glycyrrhizae radix and their respective metabolic profiles in gastrointestinal tract in vitro by HPLC-DAD and HPLC-ESI/MS analyses.[Pubmed: 23212636] |
METHODS AND RESULTS:
Two HPLC methods with diode array detection (HPLC-DAD) and electrospray ionization-mass spectrometry (HPLC-ESI/MS), respectively, were developed to investigate the differences of chemical constituents and their metabolism in gastrointestinal tract in vitro between two decoctions of crude and processed Glycyrrhizae radix. Total of eleven constituents (liquiritin apioside, liquiritin, licuraside, isoliquiritin, ononin, glycyrrhizin, liquiritigenin-7,4'-diglucoside, Licoricesaponin A3, 22β-acetoxylglycyrrhizic acid, licorice saponin G2, and yunganoside E2) were identified in the two decoctions, whereas lower contents of these constituents were usually found in the decoction of processed Glycyrrhizae Radix. [corrected] Furthermore, these constituents were metabolized into their respective aglycons in human intestinal bacteria juice, and the metabolism ratios were all higher in processed Glycyrrhizae Radix [corrected] decoction. No change was found in artificial gastric or intestinal juice.
CONCLUSIONS:
This study revealed that the processing can alter the contents of main constituents in crude G. radix and their metabolism in gastrointestinal tract, in which intestinal bacteria play an important role in the metabolism of licorice constituents. |
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