Mellein | CAS:480-33-1 | Manufacturer ChemFaces

Mellein

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Description:

Standard reference

In vitro:

Appl Environ Microbiol. 2015 Jan;81(1):177-86.

An in planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum.[Pubmed: 25326302 ]

Parastagonospora nodorum is a pathogen of wheat that affects yields globally.
METHODS AND RESULTS:
Previous transcriptional analysis identified a partially reducing polyketide synthase (PR-PKS) gene, SNOG_00477 (SN477), in P. nodorum that is highly upregulated during infection of wheat leaves. Disruption of the corresponding SN477 gene resulted in the loss of production of two compounds, which we identified as (R)-Mellein and (R)-O-methylMellein. Using a Saccharomyces cerevisiae yeast heterologous expression system, we successfully demonstrated that SN477 is the only enzyme required for the production of (R)-Mellein. This is the first identification of a fungal PKS that is responsible for the synthesis of (R)-Mellein. The P. nodorum ΔSN477 mutant did not show any significant difference from the wild-type strain in its virulence against wheat. However, (R)-Mellein at 200 μg/ml inhibited the germination of wheat (Triticum aestivum) and barrel medic (Medicago truncatula) seeds. Comparative sequence analysis identified the presence of Mellein synthase (MLNS) homologues in several Dothideomycetes and two sodariomycete genera.
CONCLUSIONS:
Phylogenetic analysis suggests that the MLNSs in fungi and bacteria evolved convergently from fungal and bacterial 6-methylsalicylic acid synthases.

J Am Chem Soc. 2012 Jul 25;134(29):11924-7.

Synthesis of (R)-mellein by a partially reducing iterative polyketide synthase.[Pubmed: 22793256]

Disruption of the corresponding SN477 gene resulted in the loss of production of two compounds, which we identified as (R)-Mellein and (R)-O-methylMellein.
METHODS AND RESULTS:
Using a Saccharomyces cerevisiae yeast heterologous expression system, we successfully demonstrated that SN477 is the only enzyme required for the production of (R)-Mellein. This is the first identification of a fungal PKS that is responsible for the synthesis of (R)-Mellein. The P. nodorum ΔSN477 mutant did not show any significant difference from the wild-type strain in its virulence against wheat. However, (R)-Mellein at 200 μg/ml inhibited the germination of wheat (Triticum aestivum) and barrel medic (Medicago truncatula) seeds. Comparative sequence analysis identified the presence of Mellein synthase (MLNS) homologues in several Dothideomycetes and two sodariomycete genera.
CONCLUSIONS:
Phylogenetic analysis suggests that the MLNSs in fungi and bacteria evolved convergently from fungal and bacterial 6-methylsalicylic acid synthases.

Mellein Description

Source:	The herbs of Goniothalamus griffithii
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage:	Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C). Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
After receiving:	The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.

Product Name	Mellein
Price:
CAS No.:	480-33-1
Catalog No.:	CFN97913
Molecular Formula:	C₁₀H₁₀O₃
Molecular Weight:	178.2 g/mol
Purity:	>=98%
Type of Compound:	Coumarins
Physical Desc.:	Powder
Source:	The herbs of Goniothalamus griffithii
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download:	COA MSDS SDF
Similar structural:	Comparison (Web) (SDF)
Guestbook:

Size /Price /Stock	10 mM * 100 uL in DMSO / Inquiry / In-stock 10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries	Coumarins Compound Library

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	5.6117 mL	28.0584 mL	56.1167 mL	112.2334 mL	140.2918 mL
5 mM	1.1223 mL	5.6117 mL	11.2233 mL	22.4467 mL	28.0584 mL
10 mM	0.5612 mL	2.8058 mL	5.6117 mL	11.2233 mL	14.0292 mL
50 mM	0.1122 mL	0.5612 mL	1.1223 mL	2.2447 mL	2.8058 mL
100 mM	0.0561 mL	0.2806 mL	0.5612 mL	1.1223 mL	1.4029 mL

CFN89298	Matsukaze-lactone	3153-73-9	350.32	C₂₀H₁₄O₆
CFN94405	7-Methoxy-4-methylcoumarin	2555-28-4	190.20	C₁₁H₁₀O₃
CFN94425	6-Methoxy-4-methylcoumarin	6295-35-8	190.20	C₁₁H₁₀O₃
CFN92538	4,6-Dimethoxy-2H-1-benzopyran-2-one	53666-78-7	206.2	C₁₁H₁₀O₄
CFN97084	4,6,7-Trimethoxy-5-methylcoumarin	62615-63-8	250.3	C₁₃H₁₄O₅
CFN96263	Edgeworin	120028-43-5	322.3	C₁₈H₁₀O₆
CFN90329	Daphnoretin	2034-69-7	352.3	C₁₉H₁₂O₇
CFN96604	Isodaphnoretin B	944824-29-7	382.32	C₂₀H₁₄O₈
CFN95207	Isoeuphorbetin	50677-55-9	354.3	C₁₈H₁₀O₈
CFN96473	Triumbelletin	131559-54-1	482.39	C₂₇H₁₄O₉