| In vivo: |
| J Pharmacol Sci. 2010;113(4):404-8. Epub 2010 Jul 13. | | Parishin C attenuates phencyclidine-induced schizophrenia-like psychosis in mice: involvements of 5-HT1A receptor.[Pubmed: 20644336] | Parishin C, a major component of Gastrodia elata BLUME (GE), was purified from GE.
Because GE modulates the serotonergic system and the 5-HT(1A) receptor is an important therapeutic target of schizophrenia, we examined whether Parishin C affects phencyclidine-induced abnormal behaviors in mice.
METHODS AND RESULTS:
Phencyclidine-induced abnormal behaviors were significantly ameliorated by Parishin C. These effects were reversed by WAY 100635, a 5HT(1A)-receptor antagonist. Consistently, Parishin C showed high affinity at 5-HT(1A) receptor as well as a 5-HT(1A)-agonist activity in a 8-OH-DPAT-stimulated [(35)S]GTP-gammaS binding assay.
CONCLUSIONS:
Our results suggest that the antipsychotic effects of Parishin C require activation of 5-HT(1A) receptors. | | Zhongguo Zhong Yao Za Zhi. 2015 Jul;40(13):2668-73. | | Study on active ingredient and mechanism in preventing vascular dementia of Tianzhusan coming from Tujia medicine.[Pubmed: 26697697] | To make clear of the absorbed components of Tianzhusan (TZS) and its possible mechanism in preventing vascular dementia (VD), the rats' models of VD were prepared by a permanent ligation of the bilateral common carotid arteries.
METHODS AND RESULTS:
After 60 days, rats were administrated with TZS for 0.1 g x kg(-1), and the volume is 0.02 mL x g(-1). After 3 days, the medicated serum was prepared and detected by UPLC, and then we predicted the possible chemical structure of the absorbed components of TZS. According to the absorbed components, the potential targets of TZS were found by ligand profiling of Discovery Studio 3.5. All of these target genes were submitted to DAVID onine for gene set enrichment analysis (GSEA). The 5 absorbed components of TZS have been predicted, and four of them have been identified as parishin B, Parishin C, parishin, pennogenin-3-O-alpha-L-rhamnopyranosy-(1-->2)-beta-D-glucoside. Through reverse finding targets, we got 861 pharmacophore models and 9 pathways from KEGG, BIOCARTA after document verification.
CONCLUSIONS:
These results showed that the efficacy mechanism of TZS on VD perhaps were be related with these absorbed components and pathways. If the traditional herbs could be proved effective by efficacy tests, the serum pharmacochemistry, computer-aided drug design, system biology and other technologies can be used in the next experiments, which will be beneficial to fast discovery of material basis and mechanisms of traditional medicine coming form ethnic minorities. |
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