SB431542 | CAS:301836-41-9 | Manufacturer ChemFaces

SB431542

ChemFaces products have been cited in many studies from excellent and top scientific journals

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* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.

According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.

Size /Price /Stock	10 mM * 1 mL in DMSO / Inquiry
Other Packaging	*Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap

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SB431542 Description

Source:
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage:	Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C). Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
After receiving:	The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.

Kinase Assay:

J Med Chem,2002 Feb 28;45(5):999-1001.

Identification of novel inhibitors of the transforming growth factor beta1 (TGF-beta1) type 1 receptor (ALK5).[Pubmed: 11855979]

SB 431542 is dissolved in DMSO at a concentration of 10 mM. The kinase domain of TGFβRI, from amino acid 200 to the C-terminus, and the full-length Smad3 protein are expressed as N-terminal glutathion S-transferase (GST) fusion proteins in the baculovirus expression system. Proteins are purified with glutathion Sepharose beads 4B. Basic FlashPlates are coated with 0.1 M sterile filtered sodium bicarbonate, pH 7.6, containing 700 ng of GST-Smad3 per 100 μL. Assay buffer contains 50 mM HEPES (pH 7.4), 5 mM MgCl2, 1 mM CaCl2, 1 mM DTT, 100 mM GTP, 3 μM ATP plus 0.5 μCi/well ɤ33P-ATP, and 85 ng of GST-ALK5 with or without SB 431542. Plates are incubated at 30 °C for 3 hours. The assay buffer is removed by aspiration, and the plate is counted on a Packard TopCount 96-well scintillation plate reader.

Cell Research:

Cancer Res, 2003 Nov 15;63(22):7791-8.

SB-431542 and Gleevec inhibit transforming growth factor-beta-induced proliferation of human osteosarcoma cells.[Pubmed: 14633705]

Cell lines:MG63 and NIH3T3
Concentrations: 0.3 μM
Incubation Time: 30 minutes
Method:
To explore the effects of ligands, MG63 and NIH3T3 cells are seeded at a density of 8 × 104 cells/well in 6-well plates and starved (0.1% FCS for MG63 cells and 0.5% FCS for NIH3T3 cells) for 24 hours before ligand stimulation. Media containing various ligands are exchanged at 48-hours intervals. Cells are trypsinized and counted by a Coulter counter on days 2, 4, and 6 after ligand stimulation. To explore the effects of constitutively active receptors, cells are seeded at a density of 2 × 105 cells/well in 6-well plates. The next day, cells are infected with adenoviruses carrying various cDNAs at a multiplicity of infection of 100. Cells are trypsinized and counted on day 3. Cell proliferation assay is performed in the presence of 0.3 μM SB 431542.

Animal Research:

Oncol Rep,2010 Dec;24(6):1637-43.

Transforming growth factor β signaling inhibitor, SB-431542, induces maturation of dendritic cells and enhances anti-tumor activity.[Pubmed: 21042762]

Animal Models: BALB/c mice receive intraperitoneal (i.p.) injections of colon-26 tumor cells.
Dosages: 1 μM solution, 100 μL/mouse
Administration: Directly injected into peritoneal cavity

Product Name	SB431542
Price:
CAS No.:	301836-41-9
Catalog No.:	CFN60029
Molecular Formula:	C₂₂H₁₆N₄O₃
Molecular Weight:	384.39 g/mol
Purity:	>=98%
Type of Compound:	Alkaloids
Physical Desc.:	Powder
Source:
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download:	COA MSDS
Similar structural:	Comparison
Guestbook:

Size /Price /Stock	10 mM * 100 uL in DMSO / Inquiry / In-stock 10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries	Alkaloids Compound Library p38MAPK Inhibitor Library

Description:	SB431542 is a potent and selective inhibitor of ALK5 with IC50 of 94 nM in a cell-free assay, 100-fold more selective for ALK5 than p38 MAPK and other kinases.
Targets:	ALK5 \| p38MAPK

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	2.6015 mL	13.0076 mL	26.0152 mL	52.0305 mL	65.0381 mL
5 mM	0.5203 mL	2.6015 mL	5.203 mL	10.4061 mL	13.0076 mL
10 mM	0.2602 mL	1.3008 mL	2.6015 mL	5.203 mL	6.5038 mL
50 mM	0.052 mL	0.2602 mL	0.5203 mL	1.0406 mL	1.3008 mL
100 mM	0.026 mL	0.1301 mL	0.2602 mL	0.5203 mL	0.6504 mL

CFN89179	Filic-3-en-25-al	147850-78-0	424.71	C₃₀H₄₈O
CFN97074	Kadsuric acid	62393-88-8	470.7	C₃₀H₄₆O₄
CFN00367	Dicrotaline	480-87-5	281.31	C₁₄H₁₉NO₅
CFN98060	Rubranol	211126-61-3	332.4	C₁₉H₂₄O₅