Kinase Assay: |
Can J Physiol Pharmacol. 1977 Apr;55(2):170-9. | Antagonism of biogenic amine-induced depression of cerebral cortical neurones by Na+, K+-ATPase in inhibitors.[Pubmed: 141320] | METHODS AND RESULTS:
The effects of iontophoretically applied Na+-, K+-dependent adenosinetriphosphatase (Na+,K+-ATPase) (EC 3.6.1.3) inhibitors (ouabain, digitoxin, digitoxigenin, strophanthin K, strophanthidin, thevetin A and Thevetin B, ethacrynate, and harmaline) on the depression of rat cerebral cortical neurones by noradrenaline, 5-hydroxytryptamine, and histamine have been studied. The inhibitors antagonized depressions of spontaneously active neurones evoked by these amines, but not those evoked by gamma-aminobutyric acid, adenosine, adenosine 5'-monophosphate, or calcium. The antagonistic potencies of the various inhibitors appeared to be proportional to their known potencies as inhibitors of Na+, K+-ATPase.
CONCLUSIONS:
The data therefore support the hypothesis that amines depress central neurones by activating an electrogenic sodium pump. |
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Structure Identification: |
J Pharm Biomed Anal. 1992 Jun;10(6):413-9. | Determination of thevetin B in serum by fluorescence polarization immunoassay.[Pubmed: 1420463] |
METHODS AND RESULTS:
Thevetin B, a cardiac glycoside of Thevetia neriifolia Juss. seeds, was determined in serum by fluorescence polarization immunoassay.
Anti-digitoxin antibody was used, Thevetin B genin being structurally identical to digitoxigenin.
CONCLUSIONS:
Cross-reactivity of 94% was found by this method, for concentrations from 5 to 80 ng ml-1. |
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