Science | Nature | Cell | View More
Natural Products
Thevetin B
Thevetin B
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Thevetin B
Price: $513 / 5mg
CAS No.: 27127-79-3
Catalog No.: CFN96147
Molecular Formula: C42H66O18
Molecular Weight: 859.0 g/mol
Purity: >=98%
Type of Compound: Steroids
Physical Desc.: Powder
Source: The herbs of Thevetia peruviana
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Download: COA    MSDS    SDF
Similar structural: Comparison (Web)  (SDF)
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $820.8 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • Imperial College London (United Kingdom)
  • Universidade do Porto (Portugal)
  • Universidad Veracuzana (Mexico)
  • University of Otago (New Zealand)
  • University of Limpopo (South Africa)
  • University of East Anglia (United Kingdom)
  • Kazusa DNA Research Institute (Japan)
  • Instituto Politécnico de Bragan?a (Portugal)
  • Harvard University (USA)
  • University of Dicle (Turkey)
  • Griffith University (Australia)
  • More...
Package
Featured Products
Panaxydol

Catalog No: CFN92797
CAS No: 72800-72-7
Price: $448/20mg
Ganoderenic acid B

Catalog No: CFN92991
CAS No: 100665-41-6
Price: $318/10mg
Matairesinol

Catalog No: CFN98983
CAS No: 580-72-3
Price: $318/10mg
Lobetyolin

Catalog No: CFN99104
CAS No: 136085-37-5
Price: $168/20mg
Vaccarin

Catalog No: CFN90131
CAS No: 53452-16-7
Price: $30/20mg
Notoginsenoside R4

Catalog No: CFN91142
CAS No: 87741-77-3
Price: $238/5mg
Shuterin

Catalog No: CFN91904
CAS No: 105377-77-3
Price: $ / mg
Strictosamide

Catalog No: CFN98237
CAS No: 23141-25-5
Price: $158/10mg
Rhamnocitrin

Catalog No: CFN97724
CAS No: 569-92-6
Price: $268/10mg
Delphinidin-3-O-galactoside chloride

Catalog No: CFN92037
CAS No: 28500-00-7
Price: $238/10mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Thevetin B is a cardiac glycoside. Thevetin A and thevetin B are Na+-, K+-dependent adenosinetriphosphatase (Na+,K+-ATPase) (EC 3.6.1.3) inhibitors.
Targets: Sodium Channel | ATPase | Potassium Channel
In vitro:
Forensic Sci Int. 2012 Feb 10;215(1-3):146-51.
Method validation of a survey of thevetia cardiac glycosides in serum samples.[Pubmed: 21376490]
A sensitive and specific liquid chromatography tandem mass spectrometry (HPLC-ESI(+)-MS/MS) procedure was developed and validated for the identification and quantification of Thevetin B and further cardiac glycosides in human serum. The seeds of Yellow Oleander (Thevetia peruviana) contain cardiac glycosides that can cause serious intoxication.
METHODS AND RESULTS:
A mixture of six thevetia glycosides was extracted from these seeds and characterized. Thevetin B, isolated and efficiently purified from that mixture, is the main component and can be used as evidence. Solid phase extraction (SPE) proved to be an effective sample preparation method. Digoxin-d3 was used as the internal standard. Although ion suppression occurs, the limit of detection (LOD) is 0.27 ng/ml serum for Thevetin B. Recovery is higher than 94%, and accuracy and precision were proficient.
CONCLUSIONS:
Method refinement was carried out with regard to developing a general screening method for cardiac glycosides. The assay is linear over the range of 0.5-8 ng/ml serum. Finally, the method was applied to a case of thevetia seed ingestion.
Thevetin B Description
Source: The herbs of Thevetia peruviana
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
3''-Galloylquercitrin

Catalog No: CFN95048
CAS No: 503446-90-0
Price: $368/5mg
Cassiaside C

Catalog No: CFN95120
CAS No: 119170-52-4
Price: $318/10mg
Polygalin J

Catalog No: CFN95175
CAS No: N/A
Price: $318/5mg
Isosecotanapartholide

Catalog No: CFN95471
CAS No: 102926-01-2
Price: $318/10mg
8-Epiloganin

Catalog No: CFN95478
CAS No: 79172-04-6
Price: $318/20mg
Toddalolactone 3'-O-methyl ether (...

Catalog No: CFN95372
CAS No: 143614-35-1
Price: $318/10mg
Momordicine II

Catalog No: CFN95167
CAS No: 91590-75-9
Price: $318/10mg
Selina-4(15),7(11)-dien-8-one

Catalog No: CFN95078
CAS No: 54707-47-0
Price: $288/10mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.1641 mL 5.8207 mL 11.6414 mL 23.2829 mL 29.1036 mL
5 mM 0.2328 mL 1.1641 mL 2.3283 mL 4.6566 mL 5.8207 mL
10 mM 0.1164 mL 0.5821 mL 1.1641 mL 2.3283 mL 2.9104 mL
50 mM 0.0233 mL 0.1164 mL 0.2328 mL 0.4657 mL 0.5821 mL
100 mM 0.0116 mL 0.0582 mL 0.1164 mL 0.2328 mL 0.291 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Kinase Assay:
Can J Physiol Pharmacol. 1977 Apr;55(2):170-9.
Antagonism of biogenic amine-induced depression of cerebral cortical neurones by Na+, K+-ATPase in inhibitors.[Pubmed: 141320]

METHODS AND RESULTS:
The effects of iontophoretically applied Na+-, K+-dependent adenosinetriphosphatase (Na+,K+-ATPase) (EC 3.6.1.3) inhibitors (ouabain, digitoxin, digitoxigenin, strophanthin K, strophanthidin, thevetin A and Thevetin B, ethacrynate, and harmaline) on the depression of rat cerebral cortical neurones by noradrenaline, 5-hydroxytryptamine, and histamine have been studied. The inhibitors antagonized depressions of spontaneously active neurones evoked by these amines, but not those evoked by gamma-aminobutyric acid, adenosine, adenosine 5'-monophosphate, or calcium. The antagonistic potencies of the various inhibitors appeared to be proportional to their known potencies as inhibitors of Na+, K+-ATPase.
CONCLUSIONS:
The data therefore support the hypothesis that amines depress central neurones by activating an electrogenic sodium pump.
Structure Identification:
J Pharm Biomed Anal. 1992 Jun;10(6):413-9.
Determination of thevetin B in serum by fluorescence polarization immunoassay.[Pubmed: 1420463]

METHODS AND RESULTS:
Thevetin B, a cardiac glycoside of Thevetia neriifolia Juss. seeds, was determined in serum by fluorescence polarization immunoassay. Anti-digitoxin antibody was used, Thevetin B genin being structurally identical to digitoxigenin.
CONCLUSIONS:
Cross-reactivity of 94% was found by this method, for concentrations from 5 to 80 ng ml-1.
Panaxydol

Catalog No: CFN92797
CAS No: 72800-72-7
Price: $448/20mg
Ganoderenic acid B

Catalog No: CFN92991
CAS No: 100665-41-6
Price: $318/10mg
Matairesinol

Catalog No: CFN98983
CAS No: 580-72-3
Price: $318/10mg
Lobetyolin

Catalog No: CFN99104
CAS No: 136085-37-5
Price: $168/20mg
Vaccarin

Catalog No: CFN90131
CAS No: 53452-16-7
Price: $30/20mg
Notoginsenoside R4

Catalog No: CFN91142
CAS No: 87741-77-3
Price: $238/5mg
Shuterin

Catalog No: CFN91904
CAS No: 105377-77-3
Price: $ / mg
Strictosamide

Catalog No: CFN98237
CAS No: 23141-25-5
Price: $158/10mg
Rhamnocitrin

Catalog No: CFN97724
CAS No: 569-92-6
Price: $268/10mg
Delphinidin-3-O-galactoside chloride

Catalog No: CFN92037
CAS No: 28500-00-7
Price: $238/10mg
Tags: buy Thevetin B | Thevetin B supplier | purchase Thevetin B | Thevetin B cost | Thevetin B manufacturer | order Thevetin B | Thevetin B distributor